{
  "abstract": "Nipah virus (NiV), a highly pathogenic agent causing severe neurologic and respiratory disease, requires Biosafety Level 4 (BSL-4) containment. While existing pseudovirus models (e.g., based on human immunodeficiency virus (HIV) or vesicular stomatitis virus (VSV) backbones) offer alternatives, their utility is often limited by reliance on a single reporter gene. Here, we developed and optimized a robust NiV pseudovirus system utilizing a VSV-ΔG backbone co-expressing dual reporters, Green Fluorescent Protein (GFP) and NanoLuciferase (NLuc). Preliminary characterization showed that VSV-ΔG-GFP/NLuc exhibited structural integrity, achieved infection rates up to 70% in susceptible cells, and demonstrated relative stability within five freeze-thaw cycles. The study on the correlation between the expression of ephrin-B2 (EFNB2) in various cell lines and the infection efficiency of pseudovirus indicates the effectiveness of the pseudovirus. Furthermore, the platform reliably detected dose-dependent neutralization by established monoclonal antibodies targeting the fusion protein and glycoprotein of NiV, yielding trends comparable to authentic virus neutralization. Collectively, these findings establish this dual-reporter NiV pseudovirus as a safe, scalable, and high-throughput tool for antiviral screening and mechanistic studies under BSL-2 conditions. The synergistic combination of GFP (enabling visual quantification and sorting) and NLuc (providing ultra-sensitive, broad dynamic range quantification) enhances both versatility and data reliability. This study not only provides a critical new tool for NiV research but also serves as a valuable reference for developing pseudovirus platforms targeting other enveloped viruses.",
  "abstract_shown": true,
  "affiliations": [
    "College of Animal Science, Fujian Agriculture and Forestry University, Fujian 350002, China.",
    "Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai 200241, China.",
    "School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.",
    "Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Yufei Tian"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Ruixin Kuang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Ruijuan Xu"
    },
    {
      "affs": [
        1
      ],
      "name": "Yang Qu"
    },
    {
      "affs": [
        1
      ],
      "name": "Ying Liao"
    },
    {
      "affs": [
        1
      ],
      "name": "Cuiping Song"
    },
    {
      "affs": [
        1
      ],
      "name": "Lei Tan"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Chan Ding"
    },
    {
      "affs": [
        1
      ],
      "name": "Xusheng Qiu"
    },
    {
      "affs": [
        1
      ],
      "name": "Yingjie Sun"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/fpcscykje0ez2.bib",
  "cited_by": 1,
  "doi": "10.52601/bpr.2025.250063",
  "fulltext_url": "https://europepmc.org/articles/PMC13740977",
  "id": "fpcscykje0ez2",
  "issns": [
    "2364-3439",
    "2364-3420"
  ],
  "landing_url": "https://europepmc.org/article/MED/42997077",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2713|Virology and Viral Diseases"
    ]
  ],
  "openalex": "W7114769345",
  "pdf_url": "https://europepmc.org/articles/PMC13740977?pdf=render",
  "pmcid": "PMC13740977",
  "pmid": "42997077",
  "published": "2026-10-31",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/fpcscykje0ez2.ris",
  "sources": [
    {
      "first_seen": "2026-11-09T15:20:34Z",
      "native_id": "MED:42997077",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42997077"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2713|Virology and Viral Diseases",
      "subfield": "epidemiology-public-health"
    }
  ],
  "title": "Design optimization and characterization of a dual-reporter Nipah virus pseudovirus.",
  "title_text": "Design optimization and characterization of a dual-reporter Nipah virus pseudovirus.",
  "type": "peer-reviewed",
  "updated_at": "2026-11-10T03:20:49Z",
  "url": "https://research.draconitic.com/paper/fpcscykje0ez2",
  "venue": "Biophysics reports",
  "versions": []
}