{
  "abstract": "Sickle cell disease (SCD) is characterized by hemolytic anaemia, painful vaso-occlusive crises, and chronic inflammation resulting partly from platelet activation. It has been shown that platelets express the nucleotide-binding domain leucine-rich repeat containing protein 3 (NLRP3) and the apoptosis-associated speck-like protein containing a CARD (ASC), forming the NLRP3 inflammasome, which controls activation of caspase-1 and cleavage of interleukin-1β (IL-1β). Bruton tyrosine kinase (BTK), known to activate the NLRP3 inflammasome, is also expressed in platelets. In SCD, platelets are activated and can secrete increased quantities of IL-1β, suggesting a possible activation of the NLRP3 inflammasome. Here, we assessed platelet P-selectin expression, caspase-1 activation, platelet-neutrophil aggregate formations by flow cytometry and BTK phosphorylation by Western blot in a cohort of 81 adult SCD patients at steady state condition (34 SC and 47 SS patients). We showed an increase in platelet activation in sickle patients and a high level of platelet-neutrophil aggregate in SS patients compared to healthy subjects (17 AA). We also demonstrated platelet caspase-1 activation in SCD patients and obtained data suggesting for the first time NLRP3 inflammasome activation in SC patients. Further studies are needed to understand the mechanism of caspase-1 activation and evaluate the potential therapeutic efficiency of Ibrutinib, a BTK inhibitor.",
  "abstract_shown": true,
  "affiliations": [
    "INSERM, BIGR, Université Paris Cité and Université des Antilles, Paris, France.",
    "Laboratoire d'Hématologie, CHU de la Guadeloupe, Pointe-à-Pitre, Guadeloupe.",
    "Service de Réanimation, CHU de la Guadeloupe, Pointe-à-Pitre, Guadeloupe.",
    "Unité Transversale de la Drépanocytose, CHU de la Guadeloupe, Hôpital Ricou, Pointe-à-Pitre, Guadeloupe.",
    "Centre d'Investigation Clinique Antilles Guyane, Inserm CIC 1424, CHU de la Guadeloupe, Pointe-à-Pitre, Guadeloupe."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Livia Claude",
      "orcid": "0000-0002-6415-3941"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Frédéric Martino",
      "orcid": "0000-0002-1389-2306"
    },
    {
      "affs": [
        0
      ],
      "name": "Lydiane Mordier"
    },
    {
      "affs": [
        0
      ],
      "name": "Doris Célanie"
    },
    {
      "affs": [
        3
      ],
      "name": "Emmanuelle Bernit",
      "orcid": "0000-0002-4492-7495"
    },
    {
      "affs": [
        0,
        3
      ],
      "name": "Maryse Etienne-Julan",
      "orcid": "0000-0003-1339-6545"
    },
    {
      "affs": [
        4
      ],
      "name": "Benoît Tressières",
      "orcid": "0000-0002-9585-4281"
    },
    {
      "affs": [
        0
      ],
      "name": "Marc Romana",
      "orcid": "0000-0002-8715-9836"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Véronique Baccini",
      "orcid": "0000-0003-3913-7664"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/fpvha203qn227.bib",
  "cited_by": 0,
  "doi": "10.1111/jcmm.71390",
  "fulltext_url": "https://europepmc.org/articles/PMC13669531",
  "id": "fpvha203qn227",
  "issns": [
    "1582-1838",
    "1582-4934"
  ],
  "landing_url": "https://europepmc.org/article/MED/42875993",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2716|Hemoglobinopathies and Related Disorders"
    ]
  ],
  "openalex": "W7221929462",
  "pdf_url": "https://europepmc.org/articles/PMC13669531?pdf=render",
  "pmcid": "PMC13669531",
  "pmid": "42875993",
  "published": "2026-10-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/fpvha203qn227.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:00Z",
      "native_id": "MED:42875993",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42875993"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2716|Hemoglobinopathies and Related Disorders",
      "subfield": "human-genetics"
    }
  ],
  "title": "Activation of Platelet Caspase-1 and P-Selectin Over-Expression in Sickle Cell Disease Patients: An Involvement of NLRP3 Inflammasome?",
  "title_text": "Activation of Platelet Caspase-1 and P-Selectin Over-Expression in Sickle Cell Disease Patients: An Involvement of NLRP3 Inflammasome?",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:00Z",
  "url": "https://research.draconitic.com/paper/fpvha203qn227",
  "venue": "Journal of cellular and molecular medicine",
  "versions": []
}