{
  "abstract": "This study evaluated the effects of esculetin on heat stress-induced splenic immunosuppression in broilers. Esculetin significantly reduced rectal temperature and respiratory rate in heat-stressed broilers (P < 0.05), improved growth performance, serum biochemical parameters, and the spleen index, and attenuated heat stress-induced splenic histopathological damage and HSP70 expression. Concurrently, esculetin elevated serum levels of immunoglobulins (IgA, IgM, and IgG) and upregulated the gene expression of anti-inflammatory cytokines, while downregulating pro-inflammatory cytokine genes (IL-1β, IL-6, and TNF-α). Network pharmacology analysis, molecular docking, and in vivo validation demonstrated that the immunomodulatory effects of esculetin were primarily mediated through targeting NF-κB, TNF-α, and IL-6, thereby modulating the NF-κB/MAPK signaling pathway. These findings provide a scientific basis for the application of esculetin as a functional feed additive to alleviate heat stress-induced immune dysfunction in poultry.",
  "abstract_shown": true,
  "affiliations": [
    "Heilongjiang Provincial Key Laboratory of Pathogenic Mechanism for Animal Disease and Comparative Medicine, Northeast Agricultural University, Harbin, 150030, China.",
    "Heilongjiang Provincial Key Laboratory of Pathogenic Mechanism for Animal Disease and Comparative Medicine, Northeast Agricultural University, Harbin, 150030, China. Electronic address: fanhonggang2002@163.com."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 17,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Jie Qi"
    },
    {
      "affs": [
        0
      ],
      "name": "Haotian Yang"
    },
    {
      "affs": [
        0
      ],
      "name": "Yang Yang"
    },
    {
      "affs": [
        0
      ],
      "name": "Xinyi Dou"
    },
    {
      "affs": [
        0
      ],
      "name": "Xin Chen"
    },
    {
      "affs": [
        0
      ],
      "name": "Qiuyue An"
    },
    {
      "affs": [
        0
      ],
      "name": "Tingyang Wu"
    },
    {
      "affs": [
        0
      ],
      "name": "Xiuzhi Liu"
    },
    {
      "affs": [
        0
      ],
      "name": "Boyuan Yu"
    },
    {
      "affs": [
        0
      ],
      "name": "Hao Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "Siqi Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "Yinkang Song"
    },
    {
      "affs": [
        0
      ],
      "name": "Chenjia Shi"
    },
    {
      "affs": [
        0
      ],
      "name": "Chao Ma"
    },
    {
      "affs": [
        0
      ],
      "name": "Jia Chen"
    },
    {
      "affs": [
        0
      ],
      "name": "Yuan Zhao"
    },
    {
      "affs": [
        1
      ],
      "name": "Honggang Fan"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/fpx412b1ay33s.bib",
  "cited_by": 0,
  "doi": "10.1016/j.psj.2026.107811",
  "fulltext_url": "https://europepmc.org/articles/PMC13695244",
  "id": "fpx412b1ay33s",
  "issns": [
    "0032-5791",
    "1525-3171"
  ],
  "landing_url": "https://europepmc.org/article/MED/42893657",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1103|Animal Nutrition and Physiology"
    ]
  ],
  "openalex": "W7213944461",
  "pdf_url": "https://europepmc.org/articles/PMC13695244?pdf=render",
  "pmcid": "PMC13695244",
  "pmid": "42893657",
  "published": "2026-09-21",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/fpx412b1ay33s.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:16Z",
      "native_id": "MED:42893657",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42893657"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1103|Animal Nutrition and Physiology",
      "subfield": "zoology-behaviour"
    }
  ],
  "title": "Dietary esculetin improves growth performance and spleen function via the NF-κB/MAPK pathway in heat-stressed broilers.",
  "title_text": "Dietary esculetin improves growth performance and spleen function via the NF-κB/MAPK pathway in heat-stressed broilers.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:16Z",
  "url": "https://research.draconitic.com/paper/fpx412b1ay33s",
  "venue": "Poultry science",
  "versions": []
}