{
  "abstract": "Background/Objectives: The 2021 World Health Organization Classification of Tumors of the Central Nervous System (WHO CNS5) integrates histological and molecular criteria for adult diffuse gliomas, but comprehensive molecular testing may be difficult to implement in resource-limited settings. This study assessed the feasibility of a sequential, marker-based approach for diagnostic reassessment and described its association with clinical characteristics and overall survival (OS) in adults from Colombia. Methods: We retrospectively analyzed 68 adults with diffuse gliomas treated at two referral hospitals in Bogotá, Colombia, between 2015 and 2023. Molecular assessment used IHC, FISH, PCR, and Sanger sequencing according to the available tissue and diagnostic workflow. The study did not include comprehensive NGS or systematic assessment of combined whole-chromosome 7 gain/chromosome 10 loss (+7/−10). Results: The diagnosis changed after molecular reassessment in 51.5% of cases. The proportion classified as glioblastoma increased from 48.5% under the historical histologic classification to 66.2% under the WHO CNS5-based algorithm, whereas FISH-defined oligodendrogliomas decreased from 17.6% to 11.8%. OS differed among historical histologic groups (p = 0.03), but not among the WHO CNS5-based groups (p = 0.13). Female sex was associated with longer OS in unadjusted analysis (p = 0.02), while age was the only independent predictor in the multivariable analysis. Conclusions: Sequential molecular reassessment materially changed diagnostic categorization in this cohort and was feasible using locally available marker-based methods. However, the results are exploratory, limited by small molecular subgroups, incomplete molecular coverage, and the retrospective treatment context; they should not be interpreted as demonstrating improved treatment selection or survival after reclassification.",
  "abstract_shown": true,
  "affiliations": [
    "School of Medicine, Universidad Nacional de Colombia, Bogotá 111321, Colombia",
    "Department of Neurosurgery, Universidad FUCS, Hospital de San José—Sociedad de Cirugía de Bogotá, Bogotá 111411, Colombia",
    "Department of Neurosurgery, Hospital Universitario Fundación Santa Fe de Bogotá, Bogotá 110111, Colombia",
    "Department of Pathology, Universidad FUCS, Hospital de San José—Sociedad de Cirugía de Bogotá, Bogotá 111411, Colombia",
    "Group of Basic Sciences in Health, Universidad FUCS, Hospital Infantil Universitario de San José, Bogotá 111221, Colombia",
    "Department of Microbiology, Universidad Nacional de Colombia, Bogotá 111321, Colombia",
    "Department of Neurosurgery, San Fernando Hospital, San Fernando B1646, Buenos Aires, Argentina",
    "School of Medicine, Universidad de Los Andes, Bogotá 111711, Colombia",
    "Dirección Académica, Universidad Nacional de Colombia, Sede de La Paz, La Paz 202010, Colombia",
    "Department of Pathology, Instituto Nacional de Cancerología, Bogotá 111511, Colombia",
    "Research Institute, Universidad FUCS, Hospital de San José—Sociedad de Cirugía de Bogotá, Bogotá 111411, Colombia"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 17,
  "authors": [
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Edgar G. Ordóñez-Rubiano",
      "orcid": "0000-0003-1179-5825"
    },
    {
      "affs": [
        3
      ],
      "name": "Fabiana Rodríguez"
    },
    {
      "affs": [
        4
      ],
      "name": "Cristian F. Siabato-Vargas"
    },
    {
      "affs": [
        4
      ],
      "name": "Luz Dary Gutiérrez-Castañeda"
    },
    {
      "affs": [
        1
      ],
      "name": "Nicolás Rincón-Arias",
      "orcid": "0009-0007-4081-5924"
    },
    {
      "affs": [
        1
      ],
      "name": "Sebastián Espinosa"
    },
    {
      "affs": [
        0,
        5
      ],
      "name": "Alba Cómbita"
    },
    {
      "affs": [
        6
      ],
      "name": "Matías Baldoncini",
      "orcid": "0000-0001-9323-8306"
    },
    {
      "affs": [
        2
      ],
      "name": "Diego F. Gómez-Amarillo"
    },
    {
      "affs": [
        2
      ],
      "name": "Fernando Hakim"
    },
    {
      "affs": [
        2,
        7
      ],
      "name": "Pedro Andrade-Andrade",
      "orcid": "0009-0005-5872-3580"
    },
    {
      "affs": [
        8
      ],
      "name": "César Payán-Gómez",
      "orcid": "0000-0002-0633-1332"
    },
    {
      "affs": [
        1
      ],
      "name": "Oscar F. Zorro-Guío"
    },
    {
      "affs": [
        1
      ],
      "name": "Javier G. Patiño-Gómez"
    },
    {
      "affs": [
        9
      ],
      "name": "Irene Riveros-Barrera"
    },
    {
      "affs": [
        3
      ],
      "name": "Oscar Mendoza"
    },
    {
      "affs": [
        9,
        10
      ],
      "name": "Rafael Parra-Medina",
      "orcid": "0000-0002-8428-5837"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/frw1h4v2q37g8.bib",
  "cited_by": 0,
  "doi": "10.3390/cancers18203364",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13702763/?tool=EBI",
  "id": "frw1h4v2q37g8",
  "issns": [
    "2072-6694"
  ],
  "landing_url": "https://www.mdpi.com/2072-6694/18/20/3364",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2716|Glioma Diagnosis and Treatment"
    ]
  ],
  "openalex": "W7204745082",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13702763/pdf/?tool=EBI",
  "pmcid": "PMC13702763",
  "pmid": null,
  "published": "2026-10-01",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/frw1h4v2q37g8.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:58Z",
      "native_id": "10.3390/cancers18203364",
      "source": "crossref",
      "url": "https://www.mdpi.com/2072-6694/18/20/3364"
    },
    {
      "first_seen": "2026-10-30T12:21:34Z",
      "native_id": "PMC:PMC13702763",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13702763"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2716|Glioma Diagnosis and Treatment",
      "subfield": "human-genetics"
    }
  ],
  "title": "Molecular Reclassification of Diffuse Gliomas: A Feasibility Study of Clinical Features and Survival Outcomes in Colombia",
  "title_text": "Molecular Reclassification of Diffuse Gliomas: A Feasibility Study of Clinical Features and Survival Outcomes in Colombia",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:21:34Z",
  "url": "https://research.draconitic.com/paper/frw1h4v2q37g8",
  "venue": "Cancers",
  "versions": []
}