{
  "abstract": "Uropathogenic Escherichia coli (UPEC) is a major cause of urinary tract infections (UTIs), with multidrug resistance (MDR) and biofilm formation complicating treatment. This study isolated and phenotypically characterized bacteriophages targeting MDR and biofilm-forming UPEC from hospital sewage in Ethiopia. Twenty-three archived MDR UPEC isolates were screened for biofilm formation, and phages were characterized by host range, efficiency of plating, multiplicity of infection (MOI), adsorption kinetics, one-step growth, environmental stability, and biofilm biomass reduction. Eight isolates (34.8%) formed biofilms, including 3 strong, 1 moderate, and 4 weak biofilm formers. Two phages, ΦET-3175 and ΦFETA-207, were isolated with titers of 1.35 × 10⁹ and 1.12 × 10⁹ PFU/mL, respectively. ΦET-3175 showed broader activity, with detectable activity against 6/23 (26.1%) isolates, an optimal MOI of 0.1, > 95% adsorption within 20 min, a latent period of approximately 30 min, and a burst size of 147 ± 4.6 PFU per infected cell. Both phages retained detectable activity across pH 4–10 and at temperatures up to 50 °C. ΦET-3175 reduced biofilm biomass by 3.2–39.5% among four susceptible biofilm-forming isolates, but the paired difference was not statistically significant (Z = − 1.826, p = 0.068). These findings provide preliminary in-vitro phenotypic evidence and support further genomic, structural, and biological characterization.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "name": "Ephrem Tamrat",
      "orcid": "0009-0006-3102-6766"
    },
    {
      "name": "Awoke Derbie",
      "orcid": "0000-0002-6949-3494"
    },
    {
      "name": "Feleke Mekonnen",
      "orcid": "0000-0003-2331-0557"
    },
    {
      "name": "Zelalem Asmare",
      "orcid": "0009-0008-8076-0179"
    },
    {
      "name": "Alene Geteneh",
      "orcid": "0000-0002-7153-4849"
    },
    {
      "name": "Tesfaye Kassa",
      "orcid": "0000-0001-5259-2245"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/ft4547a74qy66.bib",
  "cited_by": 0,
  "doi": "10.1038/s41598-026-73906-9",
  "fulltext_url": null,
  "id": "ft4547a74qy66",
  "issns": [
    "2045-2322"
  ],
  "landing_url": "https://www.nature.com/articles/s41598-026-73906-9",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2303|Bacteriophages and microbial interactions"
    ]
  ],
  "openalex": "W7215129022",
  "pdf_url": "https://www.nature.com/articles/s41598-026-73906-9_reference.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-01",
  "publisher": "Springer Science and Business Media LLC",
  "ris": "https://research.draconitic.com/paper/ft4547a74qy66.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:19Z",
      "native_id": "10.1038/s41598-026-73906-9",
      "source": "crossref",
      "url": "https://www.nature.com/articles/s41598-026-73906-9"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2303|Bacteriophages and microbial interactions",
      "subfield": "ecology"
    }
  ],
  "title": "Isolation and phenotypic characterization of bacteriophages targeting multidrug-resistant biofilm-forming uropathogenic Escherichia coli from hospital sewage",
  "title_text": "Isolation and phenotypic characterization of bacteriophages targeting multidrug-resistant biofilm-forming uropathogenic Escherichia coli from hospital sewage",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:19Z",
  "url": "https://research.draconitic.com/paper/ft4547a74qy66",
  "venue": "Scientific Reports",
  "versions": []
}