{
  "abstract": "The intestine is home to more immune cells than any other non-lymphatic peripheral tissue, but research of these cells is stymied by the technical difficulties involved in isolating them. Here, we describe a protocol to isolate highly viable immune cells from murine intestinal epithelium and lamina propria. This protocol can be used to characterize cells by flow cytometry or single-cell transcriptomics, as in Crotts et al., or by other analysis methods.",
  "abstract_shown": true,
  "affiliations": [
    "Graduate School of Biomedical Science, Mayo Clinic, Rochester, MN, USA; Department of Immunology, Mayo Clinic, Rochester, MN, USA.",
    "Graduate School of Biomedical Science, Mayo Clinic, Rochester, MN, USA; Department of Immunology, Mayo Clinic, Rochester, MN, USA. Electronic address: shapiro.virginia1@mayo.edu."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 2,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Sydney B Crotts"
    },
    {
      "affs": [
        1
      ],
      "name": "Virginia Smith Shapiro",
      "orcid": "0000-0001-9978-341X"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/ftd68zqjwcztq.bib",
  "cited_by": 0,
  "doi": "10.1016/j.xpro.2026.104864",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13698045/",
  "id": "ftd68zqjwcztq",
  "issns": [
    "2666-1667"
  ],
  "landing_url": "https://doi.org/10.1016/j.xpro.2026.104864",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Protocol"
    ],
    [
      "openalex",
      "2403|T-cell and B-cell Immunology"
    ]
  ],
  "openalex": "W7223065169",
  "pdf_url": "https://europepmc.org/articles/PMC13698045?pdf=render",
  "pmcid": "PMC13698045",
  "pmid": "42900587",
  "published": "2026-10-20",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/ftd68zqjwcztq.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:00Z",
      "native_id": "PMC13698045",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.xpro.2026.104864"
    },
    {
      "first_seen": "2026-10-30T12:19:32Z",
      "native_id": "MED:42900587",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42900587"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2403|T-cell and B-cell Immunology",
      "subfield": "immunology"
    }
  ],
  "title": "An improved protocol to isolate enriched immune cells from murine intestinal epithelium and lamina propria",
  "title_text": "An improved protocol to isolate enriched immune cells from murine intestinal epithelium and lamina propria",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:34:59Z",
  "url": "https://research.draconitic.com/paper/ftd68zqjwcztq",
  "venue": "STAR Protocols",
  "versions": []
}