{
  "abstract": "Saccharomyces boulardii (Sb) is a probiotic yeast amenable to genetic manipulation, making it a promising platform for delivery of recombinant biotherapeutics to the intestinal lumen. In this study, we engineered a recombinant Sb strain to display an anti-CD3 single-chain variable fragment (scFv) on its surface and evaluated its potential as an oral antibody delivery system. To improve antibody accessibility, we tested different stalk lengths between the scFv and the SED1 anchoring domain. The strain with the longer stalk domain (Sb649) showed more stable surface display over time and was subsequently evaluated in a dextran sulfate sodium (DSS)-induced colitis model. Sb649-treated mice showed body weight values and disease activity profiles closer to those of healthy controls. Sb649 administration was also associated with transcriptional changes, including increased Foxp3 expression and no increase in Il6 in mesenteric lymph nodes, unlike DSS and DSS+SbWT-treated mice, along with increased Muc3 expression in the colon compared to DSS-treated mice. Together, these findings show that engineered Sb can stably display antibody fragments and support its potential as a platform for oral delivery of antibody-based immunotherapies to the gastrointestinal tract.",
  "abstract_shown": true,
  "affiliations": [
    "Molecular Immunology Group, Department of Cell Biology, University of Brasília, Brasília 70910-900, DF, Brazil",
    "Molecular Biology Graduate Program, Biology Institute, University of Brasília, Brasília 70910-900, DF, Brazil",
    "Molecular Pathology Graduate Program, Faculty of Medicine, University of Brasília, Brasília 70910-900, DF, Brazil",
    "National Institute of Science and Technology of Mucosa and Skin, Belo Horizonte 31270-901, MG, Brazil"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Sylvia Barbosa Pinhate"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Pietra Oliveira Guimarães"
    },
    {
      "affs": [
        0
      ],
      "name": "Isabel Garcia Sousa",
      "orcid": "0000-0002-1916-0106"
    },
    {
      "affs": [
        0
      ],
      "name": "Manuela Maragno do Almo"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Renato Rodrigues Oliveira",
      "orcid": "0009-0002-2720-0099"
    },
    {
      "affs": [
        0
      ],
      "name": "Ana Luiza Tancredi de Matos"
    },
    {
      "affs": [
        0,
        3
      ],
      "name": "Andréa Queiroz Maranhão",
      "orcid": "0000-0002-1946-7191"
    },
    {
      "affs": [
        0,
        3
      ],
      "name": "Marcelo Macedo Brigido",
      "orcid": "0000-0002-7136-7059"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/fv7a7ran46fz5.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27208959",
  "fulltext_url": "https://europepmc.org/articles/PMC13704555",
  "id": "fv7a7ran46fz5",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://www.mdpi.com/1422-0067/27/20/8959",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2741|Monoclonal and Polyclonal Antibodies Research"
    ]
  ],
  "openalex": "W7221128900",
  "pdf_url": "https://europepmc.org/articles/PMC13704555?pdf=render",
  "pmcid": "PMC13704555",
  "pmid": "42941735",
  "published": "2026-10-09",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/fv7a7ran46fz5.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:55Z",
      "native_id": "10.3390/ijms27208959",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/20/8959"
    },
    {
      "first_seen": "2026-10-30T12:22:24Z",
      "native_id": "MED:42941735",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42941735"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2741|Monoclonal and Polyclonal Antibodies Research",
      "subfield": "imaging"
    }
  ],
  "title": "Surface Display of an Anti-CD3 scFv by Saccharomyces boulardii and Its Effect on DSS-Induced Colitis in Mice",
  "title_text": "Surface Display of an Anti-CD3 scFv by Saccharomyces boulardii and Its Effect on DSS-Induced Colitis in Mice",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:22:24Z",
  "url": "https://research.draconitic.com/paper/fv7a7ran46fz5",
  "venue": "International Journal of Molecular Sciences",
  "versions": []
}