{
  "abstract": "R-spondin1 plays a pivotal role in potentiating canonical WNT/β-catenin signaling, a pathway critical for maintaining stem cell homeostasis and driving tissue regeneration. Here, we present a scalable, serum-free protocol for producing R-spondin1-loaded small extracellular vesicles (evRSPO1) from engineered HEK293 cells, with multimodal characterization of vesicle quality and bioactivity. We also describe steps for in vitro functional validation of the vesicles using mouse intestinal organoids and for in vivo therapeutic applications in mouse intestinal regeneration via oral delivery. For complete details on the use and execution of this protocol, please refer to Yang et al.¹,².",
  "abstract_shown": true,
  "affiliations": [
    "The Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, Guangdong Province 510150, China; Guangzhou National Laboratory, Guangzhou, Guangdong Province 510005, China. Electronic address: yang_lingyan@gzlab.ac.cn.",
    "Guangzhou National Laboratory, Guangzhou, Guangdong Province 510005, China; State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin 300071, China.",
    "Guangzhou National Laboratory, Guangzhou, Guangdong Province 510005, China; Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, Guangdong Province 510080, China.",
    "The Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, Guangdong Province 510150, China; Guangzhou National Laboratory, Guangzhou, Guangdong Province 510005, China. Electronic address: chan_yunshen@gzlab.ac.cn."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Lingyan Yang"
    },
    {
      "affs": [
        1
      ],
      "name": "Ting Jiang"
    },
    {
      "affs": [
        2
      ],
      "name": "Shixiang Wang"
    },
    {
      "affs": [
        1
      ],
      "name": "Yue Yang"
    },
    {
      "affs": [
        2
      ],
      "name": "Zhiping Qiao"
    },
    {
      "affs": [
        3
      ],
      "name": "Yun-Shen Chan"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/fvn8yb5ghqn7d.bib",
  "cited_by": 0,
  "doi": "10.1016/j.xpro.2026.104891",
  "fulltext_url": "https://europepmc.org/articles/PMC13676708",
  "id": "fvn8yb5ghqn7d",
  "issns": [
    "2666-1667"
  ],
  "landing_url": "https://europepmc.org/article/MED/42848513",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Extracellular vesicles in disease"
    ]
  ],
  "openalex": "W7220935420",
  "pdf_url": "https://europepmc.org/articles/PMC13676708?pdf=render",
  "pmcid": "PMC13676708",
  "pmid": "42848513",
  "published": "2026-10-08",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/fvn8yb5ghqn7d.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:02Z",
      "native_id": "MED:42848513",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42848513"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Extracellular vesicles in disease",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Protocol for scalable production of orally deliverable R-spondin1-loaded small extracellular vesicles for WNT-signaling activation and tissue regeneration.",
  "title_text": "Protocol for scalable production of orally deliverable R-spondin1-loaded small extracellular vesicles for WNT-signaling activation and tissue regeneration.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:02Z",
  "url": "https://research.draconitic.com/paper/fvn8yb5ghqn7d",
  "venue": "STAR protocols",
  "versions": []
}