{ "abstract": "Background: Hyperuricemia and gout are closely associated with alterations in gut microbiota function. Short-chain fatty acids (SCFAs) produced by intestinal microorganisms may modulate immune responses and inflammation relevant to gout pathophysiology. This study evaluated fecal SCFA concentrations, their associations with dietary purine intake, and the relationship between Akkermansia spp. and Prevotella spp. abundance and fecal SCFA profiles across the clinical spectrum of hyperuricemia. Methods: This cross-sectional study included 380 Mexican adults: normouricemic controls (n = 141), individuals with asymptomatic hyperuricemia (AH; n = 98), and patients with gout (n = 141). Fecal SCFAs were quantified by GC-FID and bacterial abundance by qPCR. Dietary intake was assessed by semi-quantitative food frequency questionnaire and classified by purine content. Between-group differences were evaluated by the Kruskal–Wallis test (Benjamini–Hochberg correction); multivariable linear regression adjusted for age, sex, and BMI was used to assess associations, including a pre-specified dietary purine intake per clinical group interaction term. Results: Fecal acetate and propionate were significantly higher in gout patients than controls, whereas AH individuals had significantly higher butyrate. Moderate-purine food intake showed opposing associations with fecal acetate in controls (β = +3.61, p = 0.019) and gout patients (β = −4.59, p = 0.036), with a significant interaction (p = 0.004). Exploratory analyses revealed a positive association between Prevotella spp. abundance and fecal acetate exclusively in the gout group (β = 0.085, p = 0.020). Conclusion: These findings suggest distinct fecal SCFA profiles across stages of urate-related disease, with a disease-specific gut metabolic response to dietary purine intake potentially contributing to the inflammatory environment of established gout. Longitudinal studies with whole-microbiome profiling are needed to establish causality.", "abstract_shown": true, "affiliations": [ "Posgrado en Ciencias Biológicas, Universidad Nacional Autónoma de México, Unidad de Posgrado, Edificio D, 1° Piso, Circuito de Posgrados, Ciudad Universitaria, Coyoacán, Mexico City 04510, Mexico.", "Laboratorio de Gerociencias, Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra, Calz México-Xochimilco 289, Coapa, Col. Arenal de Guadalupe, Tlalpan, Mexico City 14389, Mexico.", "Departamento de Fisiología de la Nutrición, Instituto Nacional de Ciencias, Médicas y Nutrición Salvador Zubirán, Mexico City 14080, Mexico.", "Departamento de Fisiopatología Cardio-Renal, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano 1, Belisario Domínguez Secc 16, Tlalpan, Mexico City 14080, Mexico.", "Departamento de Fisiología, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano 1, Belisario Domínguez Secc 16, Tlalpan, Mexico City 14080, Mexico.", "Universidad de la Salud (UNISA), Vasco de Quiroga 1345, Sta Fé, Álvaro Obregón, Mexico City 01210, Mexico.", "Servicio de Reumatología, Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra, Calz México-Xochimilco 289, Coapa, Col. Arenal de Guadalupe, Tlalpan, Mexico City 14389, Mexico.", "Laboratorio de Microbiología Clínica, Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra, Calz México-Xochimilco 289, Coapa, Col. Arenal de Guadalupe, Tlalpan, Mexico City 14389, Mexico.", "Facultad de Ciencias de la Salud, Universidad Anáhuac Campus Sur, Av. de los Tanques 865, Torres de Potrero, Álvaro Obregón, Mexico City 01840, Mexico.", "Dental Public Health Department, Division of Graduate Studies and Research, School of Dentistry, Univesidad Nacional Autónoma de México, Mexico City 04510, Mexico.", "Departamento de Inmunología, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano 1, Belisario Domínguez Secc 16, Tlalpan, Mexico City 14080, Mexico.", "Investigadora por México, Secretaría de Ciencia, Humanidades, Tecnología e Innovación, Avenida Insurgentes Sur 1582, Crédito Constructor, Mexico City 03940, Mexico.", "Subdirección de Investigación, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano 1, Belisario Domínguez Secc 16, Tlalpan, Mexico City 14080, Mexico.", "Dirección General, Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra, Calz México-Xochimilco 289, Coapa, Col. Arenal de Guadalupe, Tlalpan, Mexico City 14389, Mexico.", "Servicio de Reumatología, Hospital General de México Eduardo Liceaga, Dr. Balmis 148, Doctores, Cuauhtémoc, Mexico City 06720, Mexico." ], "article_type": "research article", "arxiv": null, "author_total": 19, "authors": [ { "affs": [ 0 ], "name": "Alim Caelli Fuentes-Bello" }, { "affs": [ 1 ], "name": "Karla Vanessa Guevara-Gonzalez", "orcid": "0009-0006-6126-3666" }, { "affs": [ 2 ], "name": "Omar Granados-Portillo" }, { "affs": [ 3 ], "name": "Laura Gabriela Sánchez-Lozada" }, { "affs": [ 4 ], "name": "Ricardo Jasso-Chávez" }, { "affs": [ 1, 5 ], "name": "David Galeana-Cadena", "orcid": "0000-0003-1374-1361" }, { "affs": [ 6 ], "name": "Efrén Altamirano-Molina" }, { "affs": [ 1 ], "name": "Brígida Herrera-López" }, { "affs": [ 1 ], "name": "Laura Edith Martínez-Gómez", "orcid": "0000-0003-3198-1350" }, { "affs": [ 1 ], "name": "Carlos Martínez-Armenta", "orcid": "0000-0003-0413-4930" }, { "affs": [ 7 ], "name": "Luis Esaú López-Jácome", "orcid": "0000-0001-7387-0937" }, { "affs": [ 8 ], "name": "Regina Matesanz Sánchez Azcona", "orcid": "0009-0008-8711-8066" }, { "affs": [ 9 ], "name": "Mireya Martínez-García" }, { "affs": [ 10 ], "name": "Luis M Amezcua-Guerra", "orcid": "0000-0002-6258-5732" }, { "affs": [ 11, 12 ], "name": "Guadalupe Gutiérrez-Esparza", "orcid": "0000-0003-0090-2701" }, { "affs": [ 13 ], "name": "Carlos Pineda" }, { "affs": [ 14 ], "name": "Janitzia Vázquez-Mellado" }, { "affs": [ 1 ], "name": "Alberto G López-Reyes", "orcid": "0000-0002-2575-2589" }, { "affs": [ 1 ], "name": "Gabriela Angélica Martínez-Nava", "orcid": "0000-0001-9857-3840" } ], "bibtex": "https://research.draconitic.com/paper/fvnnd5v3tqx8p.bib", "cited_by": 0, "doi": "10.3390/nu18203326", "fulltext_url": "https://europepmc.org/articles/PMC13708603", "id": "fvnnd5v3tqx8p", "issns": [ "2072-6643" ], "landing_url": "https://www.mdpi.com/2072-6643/18/20/3326", "language": "eng", "licence": "CC-BY-4.0", "licence_label": "CC BY 4.0", "licence_source": "article", "licence_url": "https://creativecommons.org/licenses/by/4.0/", "metadata_licence": null, "native_subjects": [ [ "openalex", "1312|Gut microbiota and health" ] ], "openalex": "W7221548741", "pdf_url": "https://europepmc.org/articles/PMC13708603?pdf=render", "pmcid": "PMC13708603", "pmid": "42942896", "published": "2026-10-10", "publisher": "MDPI AG", "ris": "https://research.draconitic.com/paper/fvnnd5v3tqx8p.ris", "sources": [ { "first_seen": "2026-10-30T12:19:56Z", "native_id": "10.3390/nu18203326", "source": "crossref", "url": "https://www.mdpi.com/2072-6643/18/20/3326" }, { "first_seen": "2026-10-30T12:22:24Z", "native_id": "MED:42942896", "source": "europepmc", "url": "https://europepmc.org/article/MED/42942896" } ], "subjects": [ { "field": "biochemistry", "origin": "openalex:1312|Gut microbiota and health", "subfield": "molecular-biology" } ], "title": "Associations Between Gut Microbiota Abundance, Dietary Purine Intake, and Fecal Short-Chain Fatty Acids Across the Clinical Spectrum of Hyperuricemia: A Cross-Sectional Study in Mexican Adults", "title_text": "Associations Between Gut Microbiota Abundance, Dietary Purine Intake, and Fecal Short-Chain Fatty Acids Across the Clinical Spectrum of Hyperuricemia: A Cross-Sectional Study in Mexican Adults", "type": "peer-reviewed", "updated_at": "2026-10-30T12:22:24Z", "url": "https://research.draconitic.com/paper/fvnnd5v3tqx8p", "venue": "Nutrients", "versions": [] }