{
  "abstract": "CD133 is widely used as a marker of stem-like cells in hepatocellular carcinoma, yet its functional contribution remains incompletely defined. In PLC/PRF/5 cells, acute CRISPR activation of endogenous PROM1 suppresses hepatic programs, whereas PROM1 knockdown elicits an opposing hepatocyte-identity response, together defining a PROM1-responsive transcriptional axis. Single-cell profiling shows that this axis is preexisting and tracks endogenous PROM1 heterogeneity, separating a hepatocyte-like, PROM1-low compartment from a PROM1-enriched, less hepatocyte-like compartment. Sustained PROM1 activation reduces the hepatocyte-like compartment and shifts the population toward intermediate positions along this axis. Related transcriptional patterns are detectable in an independent HuH-7 dataset and a human HCC case. Endogenous ALFA tagging identifies candidate CD133-associated proteins. Together, these findings support CD133 as an active contributor to tumor-cell state redistribution, extending its role beyond a mere marker of stem-like populations.",
  "abstract_shown": true,
  "affiliations": [
    "Institute of Pathology, University of Regensburg, Regensburg, Germany"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "name": "T. Schneider"
    },
    {
      "name": "K. Kaneko"
    },
    {
      "name": "M. Vogl"
    },
    {
      "name": "K. Utpatel"
    },
    {
      "name": "A. Immel"
    },
    {
      "name": "D. Hirsch"
    },
    {
      "name": "C. Raggi"
    },
    {
      "name": "E. Spinola Lasso"
    },
    {
      "name": "M. Evert"
    },
    {
      "name": "G.-S. Feng"
    },
    {
      "name": "D. F. Calvisi"
    },
    {
      "affs": [
        0
      ],
      "name": "A. Scheiter"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/fw691y26gjjmd.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.11.01.763386",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.11.01.763386v1.full",
  "id": "fw691y26gjjmd",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.11.01.763386v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "cancer biology"
    ]
  ],
  "openalex": null,
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.11.01.763386v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/fw691y26gjjmd.ris",
  "sources": [
    {
      "first_seen": "2026-11-03T12:21:14Z",
      "native_id": "10.64898/2026.11.01.763386",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.11.01.763386v1"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "biorxiv:cancer biology",
      "subfield": "oncology"
    }
  ],
  "title": "Endogenous PROM1 activation redistributes liver cancer cells along a preexisting differentiation axis",
  "title_text": "Endogenous PROM1 activation redistributes liver cancer cells along a preexisting differentiation axis",
  "type": "preprint",
  "updated_at": "2026-11-05T00:20:43Z",
  "url": "https://research.draconitic.com/paper/fw691y26gjjmd",
  "venue": "bioRxiv",
  "versions": []
}