{
  "abstract": "Neutrophils are thought to be critical to the process whereby breast cancers establish an immunosuppressive and tumour cell nurturing ‘pre-metastatic’ niche before overt metastasis can be detected. However, the spatial localization of neutrophils and their interaction with other cell types in the lung pre-metastatic niche is not well described. We used a spontaneously metastatic mammary cancer model combined with a multiplexed three- and four-dimensional imaging approach to investigate the behaviour of neutrophils in the pre-metastatic niche. Volume fixed tissue three-dimensional imaging showed that approximately 40% of CD8 + T cells are adjacent to neutrophils at this stage. In live tissue, we found neutrophils with impaired intravascular motility congested the capillaries of pre-metastatic lungs potentially obstructing CD8 + T cells. Slowed neutrophil transit was dependent on the conformation of β2-integrin and could be recapitulated by treating non-tumour bearing mice with G-CSF, a potent systemic mediator of granulopoiesis. We found a decrease in L-selectin (CD62L) on neutrophils in the lungs of both mammary tumour bearing and G-CSF treated mice. Finally, we observed differential accumulation of intravenously injected micro-beads in the lung, suggestive of transient circulatory dead spaces which were also dependent on β2-integrin inactivation. Overall, our study proposes that integrin-mediated neutrophil congestion of the alveolar capillaries could contribute to the generation of the pulmonary pre-metastatic niche.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 25,
  "authors": [
    {
      "name": "Frédéric Fercoq"
    },
    {
      "name": "Gemma Cairns"
    },
    {
      "name": "Victoria Bridgeman"
    },
    {
      "name": "Marco de Donatis"
    },
    {
      "name": "John B.G. Mackey"
    },
    {
      "name": "Alessia Floerchinger"
    },
    {
      "name": "Amanda Mcfarlane"
    },
    {
      "name": "Judith Secklehner"
    },
    {
      "name": "Ximena Raffo-Iraolagoitia"
    },
    {
      "name": "Madeline Harrington"
    },
    {
      "name": "Lynn Mcgarry"
    },
    {
      "name": "Demi Brownlie"
    },
    {
      "name": "Declan Whyte"
    },
    {
      "name": "Lindsey W.G. Arnott"
    },
    {
      "name": "Colin Nixon"
    },
    {
      "name": "Robert Wiesheu"
    },
    {
      "name": "Anna Kilbey"
    },
    {
      "name": "Leah Brown"
    },
    {
      "name": "Sarwah Al-Khalidi"
    },
    {
      "name": "Jim Norman"
    },
    {
      "name": "Edward Roberts"
    },
    {
      "name": "Ilaria Malanchi"
    },
    {
      "name": "Karen Blyth"
    },
    {
      "name": "Seth Coffelt"
    },
    {
      "name": "Leo Carlin"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/fxdmba4fb7st6.bib",
  "cited_by": 6,
  "doi": "10.1016/j.celrep.2026.117101",
  "fulltext_url": null,
  "id": "fxdmba4fb7st6",
  "issns": [
    "2211-1247",
    "2211-1247"
  ],
  "landing_url": "https://hal.science/hal-05794263v1",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "openalex",
      "2723|Cell Adhesion Molecules Research"
    ]
  ],
  "openalex": "W7136056093",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-12",
  "publisher": "Elsevier Inc",
  "ris": "https://research.draconitic.com/paper/fxdmba4fb7st6.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:54Z",
      "native_id": "hal-05794263",
      "source": "hal",
      "url": "https://hal.science/hal-05794263v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2723|Cell Adhesion Molecules Research",
      "subfield": "immunology"
    }
  ],
  "title": "Integrin-dependent neutrophil slowing reduces lung perfusion and supports metastasis in a model of breast cancer",
  "title_text": "Integrin-dependent neutrophil slowing reduces lung perfusion and supports metastasis in a model of breast cancer",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:54Z",
  "url": "https://research.draconitic.com/paper/fxdmba4fb7st6",
  "venue": "Cell Reports",
  "versions": []
}