{
  "abstract": "Exposure to Ultraviolet-B (UV-B) light can adversely affect plant growth and cellular integrity by inducing oxidative stress and DNA damage. In this study, we investigated UV-B-induced DNA damage and repair responses in the aquatic monocotyledonous plant species Spirodela polyrhiza (duckweed). We exposed 13-day-old duckweed plantlets to broadband UV-B light for 1-10 min, followed by recovery periods of up to 24 h under normal growth conditions. We observed progressive chlorosis, wilting, and diminished plant vigor with longer durations of UV-B light exposure. Agarose gel electrophoresis demonstrated compromised genomic DNA integrity immediately after UV-B light treatment, with partial restoration of DNA quality during recovery. Immuno-slot blot assays established the accumulation of two major UV light-induced photoproducts, cyclobutane pyrimidine dimers (CPDs) and 6-4 pyrimidine-pyrimidone photoproducts [(6-4)PPs], in a dose-dependent manner following UV-B light exposure. Notably, the abundance of these DNA lesions declined substantially after recovery, indicating activation of endogenous DNA repair mechanisms. Staining with 3,3-diaminobenzidine revealed elevated accumulation of hydrogen peroxide immediately following UV-B exposure, suggesting enhanced oxidative stress. Collectively, these findings demonstrate that S. polyrhiza possesses efficient mechanisms for sensing, repairing, and mitigating DNA damage induced by oxidative stress resulting from UV-B light exposure. This study highlights the potential of duckweed as an effective model system for investigating DNA damage and repair pathways under UV-B light stress in plants.",
  "abstract_shown": true,
  "affiliations": [
    "West Virginia State University"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 2,
  "authors": [
    {
      "name": "M. A. Sanjaya"
    },
    {
      "affs": [
        0
      ],
      "name": "S. S. Patil"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/fzp8vsd6ttrae.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.02.748994",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.02.748994v1.full",
  "id": "fzp8vsd6ttrae",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.02.748994v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "plant biology"
    ],
    [
      "openalex",
      "1312|DNA Repair Mechanisms"
    ]
  ],
  "openalex": "W7207706090",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.02.748994v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-02",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/fzp8vsd6ttrae.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:05Z",
      "native_id": "10.64898/2026.09.02.748994",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.02.748994v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:plant biology",
      "subfield": "plant-biology"
    }
  ],
  "title": "DNA Damage and Repair Mechanisms in Duckweed (Spirodela polyrhiza) Under Ultraviolet-B (UV-B) Light Stress",
  "title_text": "DNA Damage and Repair Mechanisms in Duckweed (Spirodela polyrhiza) Under Ultraviolet-B (UV-B) Light Stress",
  "type": "preprint",
  "updated_at": "2026-10-30T12:19:05Z",
  "url": "https://research.draconitic.com/paper/fzp8vsd6ttrae",
  "venue": "bioRxiv",
  "versions": []
}