{
  "abstract": "The proteasome core particle (CP) can be capped by Blm10/PA200, which promotes ATP-independent degradation of disordered proteins and contributes to attenuating antigenic diversity and impacts immunotherapy upon upregulation. The assembly process that leads to Blm10-CP proteasomes remains unresolved and Blm10 is present not only at mature proteasomes but also bound to assembly intermediates. We identified and solved structures of five CP intermediate complexes by cryo-electron microscopy, two of which include Blm10. We find that CP intermediates interactions with Blm10 and Pba1/Pba2 chaperone complex are mutually exclusive and both leads to mature CP production. We further find no synergy between Pba1/Pba2 and Blm10 for CP assembly. These data suggest a parallel assembly pathway exists for Blm10 proteasomes that does not require sequential activity with Pba1/Pba2. Whereas CP affinity for Pba1/Pba2 is reduced during maturation to promote its release, no such affinity switch occurs for Blm10, further suggesting a parallel Blm10-assembly pathway for production of mature Blm10-bound CPs. Altogether, our findings reveal a functionally distinct CP assembly pathway for the production of mature Blm10-CP proteasomes.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 3901 Rainbow Blvd., HLSIC 1077, Kansas City, KS, USA.",
    "Protein Processing Section, Center for Structural Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD, USA.",
    "Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA, USA.",
    "Protein Processing Section, Center for Structural Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD, USA. kylie.walters@nih.gov.",
    "Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 3901 Rainbow Blvd., HLSIC 1077, Kansas City, KS, USA. jroelofs@kumc.edu."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Mandeep Kaur"
    },
    {
      "affs": [
        1
      ],
      "name": "Xiang Chen",
      "orcid": "0000-0002-0036-0212"
    },
    {
      "affs": [
        0
      ],
      "name": "Stella Y Lee"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Tyler M Weaver",
      "orcid": "0000-0002-5138-7140"
    },
    {
      "affs": [
        0
      ],
      "name": "Bret D Freudenthal",
      "orcid": "0000-0003-1449-4710"
    },
    {
      "affs": [
        3
      ],
      "name": "Kylie J Walters",
      "orcid": "0000-0001-7590-2891"
    },
    {
      "affs": [
        4
      ],
      "name": "Jeroen Roelofs",
      "orcid": "0000-0003-0944-2465"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/fzrstvypqqbxe.bib",
  "cited_by": 5,
  "doi": "10.1038/s41467-026-77943-w",
  "fulltext_url": "https://europepmc.org/articles/PMC13683372",
  "id": "fzrstvypqqbxe",
  "issns": [
    "2041-1723"
  ],
  "landing_url": "https://europepmc.org/article/MED/42901940",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Ubiquitin and proteasome pathways"
    ]
  ],
  "openalex": "W7213900293",
  "pdf_url": "https://europepmc.org/articles/PMC13683372?pdf=render",
  "pmcid": "PMC13683372",
  "pmid": "42901940",
  "published": "2026-09-21",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/fzrstvypqqbxe.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:41Z",
      "native_id": "MED:42901940",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42901940"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Ubiquitin and proteasome pathways",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Structure of Blm10:13S proteasome intermediate reveals parallel assembly pathways for the proteasome core particle.",
  "title_text": "Structure of Blm10:13S proteasome intermediate reveals parallel assembly pathways for the proteasome core particle.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:41Z",
  "url": "https://research.draconitic.com/paper/fzrstvypqqbxe",
  "venue": "Nature communications",
  "versions": []
}