Engineering accessory esterases into cellulosomes improves lignocellulosic biomass utilization in Ruminiclostridium cellulolyticum
Abstract
Hydroxycinnamic acids, including ferulic acid (FA) and p-coumaric acid (pCA), contribute to grass cell wall recalcitrance by decorating arabinoxylans and lignin and participating in cross-links between hemicellulose chains and within lignin–hemicellulose networks. In Ruminiclostridium cellulolyticum, the cellulosomal SGNH hydrolases SGNHh1060 and SGNHh2017 were recently identified as cinnamoyl esterases active on hydroxycinnamic acid (HCA) derivatives, whereas the intracellular esterase XuaH was involved in the processing of imported arabinoxylan-derived oligosaccharides. Here, we investigated whether increasing cinnamoyl esterase content in bacterial cellulosomes with these enzymes could improve the deconstruction of complex grass lignocellulose.
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BibTeX
@article{holmiere2026engineering,
title = {{Engineering accessory esterases into cellulosomes improves lignocellulosic biomass utilization in Ruminiclostridium cellulolyticum}},
author = {Marion Holmière and Lison Degeilh and Salomé Sauvage and Juliette Patricio and Nian Liu and Éric Record and Sandrine Pagès and Stéphanie Perret and Henri-Pierre Fierobe and Nicolas Vita},
journal = {Journal of Biological Engineering},
year = {2026},
month = aug,
doi = {10.1186/s13036-026-00764-4},
url = {https://hal.science/hal-05762245v1},
note = {CC BY-NC-ND 4.0},
}
RIS
TY - JOUR
TI - Engineering accessory esterases into cellulosomes improves lignocellulosic biomass utilization in Ruminiclostridium cellulolyticum
AU - Marion Holmière
AU - Lison Degeilh
AU - Salomé Sauvage
AU - Juliette Patricio
AU - Nian Liu
AU - Éric Record
AU - Sandrine Pagès
AU - Stéphanie Perret
AU - Henri-Pierre Fierobe
AU - Nicolas Vita
PY - 2026
DA - 2026/08/22
JO - Journal of Biological Engineering
DO - 10.1186/s13036-026-00764-4
UR - https://hal.science/hal-05762245v1
AB - Hydroxycinnamic acids, including ferulic acid (FA) and p-coumaric acid (pCA), contribute to grass cell wall recalcitrance by decorating arabinoxylans and lignin and participating in cross-links between hemicellulose chains and within lignin–hemicellulose networks. In Ruminiclostridium cellulolyticum, the cellulosomal SGNH hydrolases SGNHh1060 and SGNHh2017 were recently identified as cinnamoyl esterases active on hydroxycinnamic acid (HCA) derivatives, whereas the intracellular esterase XuaH was involved in the processing of imported arabinoxylan-derived oligosaccharides. Here, we investigated whether increasing cinnamoyl esterase content in bacterial cellulosomes with these enzymes could improve the deconstruction of complex grass lignocellulose.
SN - 1754-1611
ER -