Association of Circulating microRNAs in Plasma with Coronary Collateral Circulation in Patients with Chronic Total Occlusion
- 1National Medical Research Center for Therapy and Preventive Medicine, Ministry of Healthcare of the Russian Federation, Petroverigsky Per. 10, Bld. 3, Moscow 101000, Russia
- 2Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 1-73, Leninskie Gory, Moscow 119991, Russia
Abstract
Chronic total occlusion (CTO) occurs in 16–52% of patients with coronary artery disease (CAD) and is often accompanied by variable development of coronary collateral circulation (CCC). The aim of this work was to study the associations between circulating plasma microRNAs (miRNAs) and the development of CCC in patients with CTO. This exploratory study included 43 patients: a control group—patients without significant coronary artery stenosis (n = 20), and two CTO groups with good (n = 13) and poor (n = 10) CCC based on the Rentrop classification. The study was performed simultaneously on two types of plasma samples (K2EDTA and sodium citrate). Total RNA was isolated using the miRNeasy Serum/Plasma Advanced Kit. Libraries were prepared using the QIAseq miRNA UDI Library Kit and then sequenced on the NextSeq 550 using a 1 × 75 bp protocol. A total of 18 miRNAs were found to be differentially expressed (DE) in EDTA plasma and 13 in citrate plasma; only one miRNA in citrate plasma (hsa-miR-92a-3p) was differentially expressed between the good and poor CCC groups. However, given the limited sample size, the findings should be considered hypothesis-generating, and further studies are required for validation in larger, independent cohorts and to establish the roles of these miRNAs in CCC development.
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BibTeX
@article{kiseleva2026association,
title = {{Association of Circulating microRNAs in Plasma with Coronary Collateral Circulation in Patients with Chronic Total Occlusion}},
author = {Anna Kiseleva and Evgeniia Sotnikova and Anastasia Zharikova and Dmitry Vasilev and Vladimir Kutsenko and Anna Borisova and Alexandra Ershova and Alexey Meshkov and Oxana Drapkina},
journal = {International Journal of Molecular Sciences},
year = {2026},
month = oct,
doi = {10.3390/ijms27209116},
url = {https://www.mdpi.com/1422-0067/27/20/9116},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Association of Circulating microRNAs in Plasma with Coronary Collateral Circulation in Patients with Chronic Total Occlusion
AU - Anna Kiseleva
AU - Evgeniia Sotnikova
AU - Anastasia Zharikova
AU - Dmitry Vasilev
AU - Vladimir Kutsenko
AU - Anna Borisova
AU - Alexandra Ershova
AU - Alexey Meshkov
AU - Oxana Drapkina
PY - 2026
DA - 2026/10/14
JO - International Journal of Molecular Sciences
DO - 10.3390/ijms27209116
UR - https://www.mdpi.com/1422-0067/27/20/9116
AB - Chronic total occlusion (CTO) occurs in 16–52% of patients with coronary artery disease (CAD) and is often accompanied by variable development of coronary collateral circulation (CCC). The aim of this work was to study the associations between circulating plasma microRNAs (miRNAs) and the development of CCC in patients with CTO. This exploratory study included 43 patients: a control group—patients without significant coronary artery stenosis (n = 20), and two CTO groups with good (n = 13) and poor (n = 10) CCC based on the Rentrop classification. The study was performed simultaneously on two types of plasma samples (K2EDTA and sodium citrate). Total RNA was isolated using the miRNeasy Serum/Plasma Advanced Kit. Libraries were prepared using the QIAseq miRNA UDI Library Kit and then sequenced on the NextSeq 550 using a 1 × 75 bp protocol. A total of 18 miRNAs were found to be differentially expressed (DE) in EDTA plasma and 13 in citrate plasma; only one miRNA in citrate plasma (hsa-miR-92a-3p) was differentially expressed between the good and poor CCC groups. However, given the limited sample size, the findings should be considered hypothesis-generating, and further studies are required for validation in larger, independent cohorts and to establish the roles of these miRNAs in CCC development.
SN - 1422-0067
ER -