Hierarchical PLA/chitosan/AgNPs composites with architecture-dependent antibacterial activity and preliminary ecotoxicological assessment
Abstract
Biocompatible composites with antibacterial functionality are increasingly sought to reduce plastic dependence and microbial contamination. Here, we report the fabrication of a composite by electrospinning poly(lactic acid) (PLA) nanofibers onto chitosan (CH) films embedded with green-synthesized silver nanoparticles (AgNPs). Physicochemical analyses confirmed integration: FTIR spectra indicated hydrogen bonding between PLA and CH, while XRD patterns revealed decreased crystallinity and amorphous domains. SEM micrographs showed a compact PLA-fiber-coated CH matrix, and the SEIRA effect suggested close proximity between PLA and AgNPs. Thermogravimetric analysis indicated intermediate thermal stability (∼235 °C). Antibacterial activity, assessed by the Bauer–Kirby disk diffusion method against Staphylococcus aureus and Escherichia coli (n = 3), showed that CH films with AgNPs (PCH-AgNPs) achieved the largest inhibition areas (1.767 ± 0.510 mm 2 and 1.760 ± 0.723 mm 2 , respectively), representing an increase of 14–20% over CH films without nanoparticles. PLA-coated composites (PCH-AgNPs/FPLA) exhibited moderate but sustained activity (1.703 ± 0.367 mm 2 for S. aureus and 1.027 ± 0.437 mm 2 for E. coli ), consistent with the FPLA layer modulating total silver availability and antibacterial intensity, as assessed by MP-AES monitoring of total silver diffusion over 30 days. Preliminary ecotoxicological screening using Daphnia sp . and Lactuca sativa showed no treatment-associated increase relative to controls under the single conditions evaluated, without significant inhibition of seed germination (70%), although minor sublethal effects on radicle and hypocotyl elongation were observed, especially in composites without AgNPs. The PCH-AgNPs/FPLA composite combines antibacterial efficacy, thermal stability, and preliminary ecotoxicological compatibility, showing promise for biomedical and environmentally sustainable applications.
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BibTeX
@article{calderonayala2026hierarchical,
title = {{Hierarchical PLA/chitosan/AgNPs composites with architecture-dependent antibacterial activity and preliminary ecotoxicological assessment}},
author = {Luisa Elizabeth Calderón-Ayala and Jose Cconislla Bello and Arthur Reymond and Almudena Marti and Segundo Jáuregui-Rosas and Angélica María Baena-Moncada and Roxana Yesenia Pastrana-Alta},
journal = {Next Materials},
year = {2026},
month = sep,
doi = {10.1016/j.nxmate.2026.103447},
url = {https://hal.science/hal-05749107v1},
note = {CC BY},
}
RIS
TY - JOUR
TI - Hierarchical PLA/chitosan/AgNPs composites with architecture-dependent antibacterial activity and preliminary ecotoxicological assessment
AU - Luisa Elizabeth Calderón-Ayala
AU - Jose Cconislla Bello
AU - Arthur Reymond
AU - Almudena Marti
AU - Segundo Jáuregui-Rosas
AU - Angélica María Baena-Moncada
AU - Roxana Yesenia Pastrana-Alta
PY - 2026
DA - 2026/09/14
JO - Next Materials
DO - 10.1016/j.nxmate.2026.103447
UR - https://hal.science/hal-05749107v1
AB - Biocompatible composites with antibacterial functionality are increasingly sought to reduce plastic dependence and microbial contamination. Here, we report the fabrication of a composite by electrospinning poly(lactic acid) (PLA) nanofibers onto chitosan (CH) films embedded with green-synthesized silver nanoparticles (AgNPs). Physicochemical analyses confirmed integration: FTIR spectra indicated hydrogen bonding between PLA and CH, while XRD patterns revealed decreased crystallinity and amorphous domains. SEM micrographs showed a compact PLA-fiber-coated CH matrix, and the SEIRA effect suggested close proximity between PLA and AgNPs. Thermogravimetric analysis indicated intermediate thermal stability (∼235 °C). Antibacterial activity, assessed by the Bauer–Kirby disk diffusion method against Staphylococcus aureus and Escherichia coli (n = 3), showed that CH films with AgNPs (PCH-AgNPs) achieved the largest inhibition areas (1.767 ± 0.510 mm 2 and 1.760 ± 0.723 mm 2 , respectively), representing an increase of 14–20% over CH films without nanoparticles. PLA-coated composites (PCH-AgNPs/FPLA) exhibited moderate but sustained activity (1.703 ± 0.367 mm 2 for S. aureus and 1.027 ± 0.437 mm 2 for E. coli ), consistent with the FPLA layer modulating total silver availability and antibacterial intensity, as assessed by MP-AES monitoring of total silver diffusion over 30 days. Preliminary ecotoxicological screening using Daphnia sp . and Lactuca sativa showed no treatment-associated increase relative to controls under the single conditions evaluated, without significant inhibition of seed germination (70%), although minor sublethal effects on radicle and hypocotyl elongation were observed, especially in composites without AgNPs. The PCH-AgNPs/FPLA composite combines antibacterial efficacy, thermal stability, and preliminary ecotoxicological compatibility, showing promise for biomedical and environmentally sustainable applications.
SN - 2949-8228
ER -