A single-nucleus and spatial transcriptomic atlas of the Syrian hamster preoptic area across hibernation states
- 1RIKEN Center for Biosystems Dynamics Research
Abstract
Hibernation is an extreme physiological state in certain mammals that is characterized by a reversible reduction in metabolic and thermogenic demands. Although the preoptic area has been implicated in the regulation of hibernation, comprehensive molecular and spatial resources for this brain region in hibernating mammals remain limited. Here, we present an integrated transcriptomic resource of the preoptic area generated using single-nucleus RNA sequencing (snRNA-seq) and Xenium-based spatial transcriptomics in a mammalian hibernator, Syrian hamster. The resource includes cross-species transcriptomic annotations relative to mouse preoptic area datasets, seasonal snRNA-seq datasets obtained under non-hibernating and hibernating conditions, and spatially resolved cell-type annotations generated from Xenium profiling. Together, these datasets provide a reference framework for identifying neuronal populations, examining molecular conservation across species and conditions, and integrating transcriptomic identity with spatial organization.
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BibTeX
@misc{sato2026singlenucleus,
title = {{A single-nucleus and spatial transcriptomic atlas of the Syrian hamster preoptic area across hibernation states}},
author = {H. Sato and H. Sugimoto and C. Shao and A. Yamauchi and M. Tsurutani and T. Goto and M. Hagihara and D. Cho and N. Shimada and M. Kadota and T. Kondo and S. Kuroda and G. A. Sunagawa and H. Kiyonari and Y. Yamaguchi and K. Miyamichi},
howpublished = {bioRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.04.749515},
url = {https://www.biorxiv.org/content/10.64898/2026.09.04.749515v1},
note = {CC BY-NC},
}
RIS
TY - UNPB
TI - A single-nucleus and spatial transcriptomic atlas of the Syrian hamster preoptic area across hibernation states
AU - H. Sato
AU - H. Sugimoto
AU - C. Shao
AU - A. Yamauchi
AU - M. Tsurutani
AU - T. Goto
AU - M. Hagihara
AU - D. Cho
AU - N. Shimada
AU - M. Kadota
AU - T. Kondo
AU - S. Kuroda
AU - G. A. Sunagawa
AU - H. Kiyonari
AU - Y. Yamaguchi
AU - K. Miyamichi
PY - 2026
DA - 2026/09/04
PB - bioRxiv
DO - 10.64898/2026.09.04.749515
UR - https://www.biorxiv.org/content/10.64898/2026.09.04.749515v1
AB - Hibernation is an extreme physiological state in certain mammals that is characterized by a reversible reduction in metabolic and thermogenic demands. Although the preoptic area has been implicated in the regulation of hibernation, comprehensive molecular and spatial resources for this brain region in hibernating mammals remain limited. Here, we present an integrated transcriptomic resource of the preoptic area generated using single-nucleus RNA sequencing (snRNA-seq) and Xenium-based spatial transcriptomics in a mammalian hibernator, Syrian hamster. The resource includes cross-species transcriptomic annotations relative to mouse preoptic area datasets, seasonal snRNA-seq datasets obtained under non-hibernating and hibernating conditions, and spatially resolved cell-type annotations generated from Xenium profiling. Together, these datasets provide a reference framework for identifying neuronal populations, examining molecular conservation across species and conditions, and integrating transcriptomic identity with spatial organization.
ER -