Skip to content
DraconiticResearch
Search Sources API
197,693 studies31 sourcesUpdated 31 min ago
Peer-reviewedCC BY 4.0PLOS One

Integrated analysis of transcriptomic and proteomic profiles identifies divergent regulatory networks for intramuscular fat in Chinese versus Western Pigs

  • Caoyan Xu1
  • Pengcheng Shen2
  • Chang Sheng Jiang3
  • Ahmed H. Ghonaim4
  • Man Ren5
  • Xiaojin Li6
  • Qianqian Hu
  • Chonglong Wang
6 affiliations
  1. 1College of Animal Science, Anhui Science and Technology University, Chuzhou, China
  2. 2Anhui Province Key Laboratory of Animal Nutritional Regulation and Health, Chuzhou, China
  3. 3Anhui Engineering Technology Research Center of Pork Quality Control and Enhance, Chuzhou, China
  4. 4National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
  5. 5Desert Research Center, Cairo, Egypt
  6. 6Institute of Animal Husbandry and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei, China
Open at sourcePDFDOICite

Abstract

Intramuscular fat (IMF) content is a key determinant of pork quality. In this study, the Chinese indigenous Huai pig (a fat-type breed) and the Western Duroc pig (a lean-type breed) were selected to investigate the molecular mechanisms underlying IMF deposition. Proteomic analysis identified 91 differentially expressed proteins (DEPs) between the two breeds. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that several key lipid metabolism-related pathways were significantly enriched, including the Lipid metabolic process, AMPK signaling pathway, nicotinate and nicotinamide metabolism, and Fat digestion and absorption. Western blotting further confirmed the reliability of the proteomic results. Integrated transcriptomic and proteomic analyses highlighted significant enrichment of the PPAR signaling pathway, which plays a central role in lipid metabolism. These findings suggest that enhanced fatty-acid uptake contributes to increased lipid deposition in muscle tissue. Additionally, several potential candidate genes associated with IMF deposition were identified, including CD36, SCARB2, ACADVL, ACADSB, ACADM, UNC119B, PAS-4, and APOA1. Collectively, this study provides valuable insights into the molecular regulation of IMF deposition in pigs and offers a theoretical basis and genetic resources for improving pork quality and advancing molecular breeding.

Related

  1. Integrated Spatial and Bulk Transcriptomic Analysis Identifies a Multicellular Ecosystem Involved in Progression of Young Breast Cancer.
    Xiaolin Chen, Zhuozhi Liang, Xinming Chen +5
    Cancer medicinePeer-reviewedCC BY-NC-NDFull textMolecular biology & gene regulationEurope PMCDOAJ
  2. Integrated transcriptomic analysis identifies an AKT1-associated metabolic-inflammatory signature in osteoporosis.
    Chenglong Bao, Ronggui Hu
    Clinical and experimental medicinePeer-reviewedCC BY-NC-NDFull textMolecular biology & gene regulationEurope PMC
  3. High-Throughput Plasma Proteomic Profiling Identifies Novel Biomarker Candidates in Parkinson’s Disease with Cognitive Decline
    Shervin Khosousi, Latha Velayudhan, Xiaoping Yang +4
    Molecular & Cellular ProteomicsPeer-reviewedLicence not statedProteomics & metabolomicsCrossref
  4. A Novel Proteomic Approach Identifies New Interaction Partners for Proliferating Cell Nuclear Antigen
    Laurence Meslet-Cladiére, Cédric Norais, Joëlle Kuhn +6
    Journal of Molecular BiologyPeer-reviewedLicence not statedFull text54 citedMolecular biology & gene regulationHAL
  5. 1120 Hyperplex spatial profiling of transcriptomic, proteomic, and protein–protein proximity layers for an in-depth characterization of the tumor microenvironment
    Pino Bordignon, Saska Brajkovic, Benjamin Pelz +6
    Journal for ImmunoTherapy of CancerPeer-reviewedCC BYMolecular biology & gene regulationDOAJ

Cite

BibTeXRIS
BibTeX
@article{xu2026integrated,
  title = {{Integrated analysis of transcriptomic and proteomic profiles identifies divergent regulatory networks for intramuscular fat in Chinese versus Western Pigs}},
  author = {Caoyan Xu and Pengcheng Shen and Chang Sheng Jiang and Ahmed H. Ghonaim and Man Ren and Xiaojin Li and Qianqian Hu and Chonglong Wang},
  journal = {PLOS One},
  year = {2026},
  month = sep,
  doi = {10.1371/journal.pone.0354756},
  url = {https://doi.org/10.1371/journal.pone.0354756},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Integrated analysis of transcriptomic and proteomic profiles identifies divergent regulatory networks for intramuscular fat in Chinese versus Western Pigs
AU  - Caoyan Xu
AU  - Pengcheng Shen
AU  - Chang Sheng Jiang
AU  - Ahmed H. Ghonaim
AU  - Man Ren
AU  - Xiaojin Li
AU  - Qianqian Hu
AU  - Chonglong Wang
PY  - 2026
DA  - 2026/09/18
JO  - PLOS One
DO  - 10.1371/journal.pone.0354756
UR  - https://doi.org/10.1371/journal.pone.0354756
AB  - Intramuscular fat (IMF) content is a key determinant of pork quality. In this study, the Chinese indigenous Huai pig (a fat-type breed) and the Western Duroc pig (a lean-type breed) were selected to investigate the molecular mechanisms underlying IMF deposition. Proteomic analysis identified 91 differentially expressed proteins (DEPs) between the two breeds. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that several key lipid metabolism-related pathways were significantly enriched, including the Lipid metabolic process, AMPK signaling pathway, nicotinate and nicotinamide metabolism, and Fat digestion and absorption. Western blotting further confirmed the reliability of the proteomic results. Integrated transcriptomic and proteomic analyses highlighted significant enrichment of the PPAR signaling pathway, which plays a central role in lipid metabolism. These findings suggest that enhanced fatty-acid uptake contributes to increased lipid deposition in muscle tissue. Additionally, several potential candidate genes associated with IMF deposition were identified, including CD36, SCARB2, ACADVL, ACADSB, ACADM, UNC119B, PAS-4, and APOA1. Collectively, this study provides valuable insights into the molecular regulation of IMF deposition in pigs and offers a theoretical basis and genetic resources for improving pork quality and advancing molecular breeding.
SN  - 1932-6203
ER  -