Chemical and Biological Mobilization of Soil Phosphorus by ppk-Expressing Transgenic Rice (Oryza sativa L.).
- 1State Key Laboratory of Water Pollution Control and Green Resource Recycling, Key Laboratory of Aquatic Ecosystem Health in the Middle and Lower Reaches of Yangtze River of Ministry of Ecology and Environment, School of Environment, Nanjing University, Nanjing 210023, China.
Abstract
Phosphorus (P) availability in alkaline soils is constrained by the immobilization of legacy P, particularly through calcium phosphate precipitation. We used a confined soil-plant microcosm to examine how expression of the polyphosphate kinase gene (ppk) is associated with legacy P utilization in rice (Oryza sativa L.). We compared ppk-expressing transgenic rice (ETR) carrying single-copy (ETRS) and double-copy (ETRD) insertions with their isogenic wild type (WT), without P fertilization. Both transgenic lines had higher root organic acid release and acid phosphatase activity on day 30 and greater plant P acquisition by day 60 than WT; soil pH declined to a greater extent. On day 30, transgenic roots released more oxalate and malate, and their exudates mobilized more soil P than WT exudates in a separate incubation assay. These traits corresponded to greater depletion of the operationally defined HCl-P and NaOH-P pools. Bacterial profiles and PICRUSt2 predictions were descriptive because one pooled library per genotype precluded tests of genotype effects. Diffusive gradients in thin films (DGT) measurements revealed greater rhizosphere labile P in the ETRD early in plant growth, followed by a decline as plant P concentrations increased. This pattern was consistent with an early increase in P supply followed by plant-driven depletion. Net P acquisition in ETRD was 2.55 times that in WT, and ETRD exhibited 53% greater root biomass, while ETRS showed intermediate responses. Collectively, these results link enhanced plant P acquisition to greater P release in exudate incubation assays and higher acid phosphatase activity within this confined system.
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BibTeX
@article{yang2026chemical,
title = {{Chemical and Biological Mobilization of Soil Phosphorus by ppk-Expressing Transgenic Rice (Oryza sativa L.).}},
author = {Shunqing Yang and Mengmeng Wang and Xiaohua Song and Chenyu Wang and Yunfan Zhang and Di He and Jingyun Yin and Xiaotong Ge and Liyu Pan and Minxiang Xu and Yong'an Wei and Liuyan Yang},
journal = {Plants (Basel, Switzerland)},
year = {2026},
month = oct,
doi = {10.3390/plants15203098},
url = {https://europepmc.org/article/MED/42943051},
note = {CC BY},
}
RIS
TY - JOUR
TI - Chemical and Biological Mobilization of Soil Phosphorus by ppk-Expressing Transgenic Rice (Oryza sativa L.).
AU - Shunqing Yang
AU - Mengmeng Wang
AU - Xiaohua Song
AU - Chenyu Wang
AU - Yunfan Zhang
AU - Di He
AU - Jingyun Yin
AU - Xiaotong Ge
AU - Liyu Pan
AU - Minxiang Xu
AU - Yong'an Wei
AU - Liuyan Yang
PY - 2026
DA - 2026/10/10
JO - Plants (Basel, Switzerland)
DO - 10.3390/plants15203098
UR - https://europepmc.org/article/MED/42943051
AB - Phosphorus (P) availability in alkaline soils is constrained by the immobilization of legacy P, particularly through calcium phosphate precipitation. We used a confined soil-plant microcosm to examine how expression of the polyphosphate kinase gene (ppk) is associated with legacy P utilization in rice (Oryza sativa L.). We compared ppk-expressing transgenic rice (ETR) carrying single-copy (ETRS) and double-copy (ETRD) insertions with their isogenic wild type (WT), without P fertilization. Both transgenic lines had higher root organic acid release and acid phosphatase activity on day 30 and greater plant P acquisition by day 60 than WT; soil pH declined to a greater extent. On day 30, transgenic roots released more oxalate and malate, and their exudates mobilized more soil P than WT exudates in a separate incubation assay. These traits corresponded to greater depletion of the operationally defined HCl-P and NaOH-P pools. Bacterial profiles and PICRUSt2 predictions were descriptive because one pooled library per genotype precluded tests of genotype effects. Diffusive gradients in thin films (DGT) measurements revealed greater rhizosphere labile P in the ETRD early in plant growth, followed by a decline as plant P concentrations increased. This pattern was consistent with an early increase in P supply followed by plant-driven depletion. Net P acquisition in ETRD was 2.55 times that in WT, and ETRD exhibited 53% greater root biomass, while ETRS showed intermediate responses. Collectively, these results link enhanced plant P acquisition to greater P release in exudate incubation assays and higher acid phosphatase activity within this confined system.
SN - 2223-7747
ER -