Influence of Non-Biodegradable ZrO2 and Y2O3 Nanoparticles on the Genomic and Redox Status of Chicken Embryo
5 affiliations
- 1Department of Physiological Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences—SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland
- 2Institute of Physics, Polish Academy of Sciences, Al. Lotników 32/46, 02-668 Warsaw, Poland
- 3Department of Botany and Plant Physiology, Institute of Biology, Warsaw University of Life Sciences—SGGW, 02-776 Warsaw, Poland
- 4Scientific Society of Veterinary Medicine Students, Warsaw University of Life Sciences—SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland
- 5Division of Microbiology, Department of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences—SGGW, Ciszewskiego 8, 02-786 Warsaw, Poland
Abstract
With the growing use of nanotechnology, the issue of exposure to non-biodegradable nanomaterials is becoming increasingly significant, raising concerns about their potential impact on developing organisms. Their use in dentistry, optoelectronics and biomedicine leads to environmental exposure. Zirconium oxide (ZrO2) and yttrium oxide (Y2O3) nanoparticles are chemically stable and poorly soluble materials; however, their embryotoxicity remains extremely poorly characterised. This study assessed the effect of ZrO2 and Y2O3 nanoparticles on the development of chicken embryos. The nanoparticles were administered into the air chamber of the egg on the first day of incubation at concentrations of 10 and 100 µg/mL. The redox status was assessed based on superoxide dismutase activity, malondialdehyde content and the level of carbonylated proteins. Transcriptomic responses were evaluated using a whole-genome microarray, and selected genes were verified by qPCR. Both types of nanoparticles induced time-dependent changes in markers of oxidative stress. Transcriptomic analysis revealed distinctly different responses, with seven genes showing altered expression following ZrO2 administration, compared with 555 following Y2O3 administration. These results indicate that non-biodegradable metal oxide nanoparticles can influence the redox balance and gene expression during early embryonic development, with the extent of the response depending on the type of nanoparticle.
Related
- Influence of PEGylation and Surface Charge on Biodistribution of Shortwave Infrared Emitting Nanoparticles for In Vivo Bioimaging
- Influence of Drying Methods on Phytochemical Retention of Moringa oleifera Leaves and Green Synthesis of Zinc Oxide Nanoparticles Using the Optimal Moringa oleifera Leaf Extract
- Gamma-irradiation applied to the synthesis of selenium nanoparticles and its influence on physicochemical properties
- Cobalt oxide nanoparticles disrupt cellular homeostasis through oxidative genomic instability and mitochondrial stress, driving necrosis in non-small lung cancer cells
- Valorization of Agro-Industrial By-Products in the Development of Bio-Polymer Films, Biodegradable Plasticulture, and Intelligent Food-Packaging Materials
BibTeX
@article{dominiak2026influence,
title = {{Influence of Non-Biodegradable ZrO2 and Y2O3 Nanoparticles on the Genomic and Redox Status of Chicken Embryo}},
author = {Bartłomiej Dominiak and Julita Rosowska and Agnieszka Wal and Alicja Majewska and Lena M. Rudy and Bartłomiej S. Witkowski and Łukasz Wachnicki and Jarosław Kaszewski and Anna Słońska and Joanna Cymerys and Mikołaj A. Gralak and Marek Godlewski and Michał M. Godlewski},
journal = {Nanomaterials},
year = {2026},
month = oct,
doi = {10.3390/nano16211347},
url = {https://www.mdpi.com/2079-4991/16/21/1347},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Influence of Non-Biodegradable ZrO2 and Y2O3 Nanoparticles on the Genomic and Redox Status of Chicken Embryo
AU - Bartłomiej Dominiak
AU - Julita Rosowska
AU - Agnieszka Wal
AU - Alicja Majewska
AU - Lena M. Rudy
AU - Bartłomiej S. Witkowski
AU - Łukasz Wachnicki
AU - Jarosław Kaszewski
AU - Anna Słońska
AU - Joanna Cymerys
AU - Mikołaj A. Gralak
AU - Marek Godlewski
AU - Michał M. Godlewski
PY - 2026
DA - 2026/10/23
JO - Nanomaterials
DO - 10.3390/nano16211347
UR - https://www.mdpi.com/2079-4991/16/21/1347
AB - With the growing use of nanotechnology, the issue of exposure to non-biodegradable nanomaterials is becoming increasingly significant, raising concerns about their potential impact on developing organisms. Their use in dentistry, optoelectronics and biomedicine leads to environmental exposure. Zirconium oxide (ZrO2) and yttrium oxide (Y2O3) nanoparticles are chemically stable and poorly soluble materials; however, their embryotoxicity remains extremely poorly characterised. This study assessed the effect of ZrO2 and Y2O3 nanoparticles on the development of chicken embryos. The nanoparticles were administered into the air chamber of the egg on the first day of incubation at concentrations of 10 and 100 µg/mL. The redox status was assessed based on superoxide dismutase activity, malondialdehyde content and the level of carbonylated proteins. Transcriptomic responses were evaluated using a whole-genome microarray, and selected genes were verified by qPCR. Both types of nanoparticles induced time-dependent changes in markers of oxidative stress. Transcriptomic analysis revealed distinctly different responses, with seven genes showing altered expression following ZrO2 administration, compared with 555 following Y2O3 administration. These results indicate that non-biodegradable metal oxide nanoparticles can influence the redox balance and gene expression during early embryonic development, with the extent of the response depending on the type of nanoparticle.
SN - 2079-4991
ER -