Consistent functional properties of MSC-exosomes from different batches revealed by comparative multi-omics, bioinformatics and functional tests
- 1ExoCoBio Exosome Institute (EEI), ExoCoBio Inc, STE 306, 19 Gasan Digital 1-Ro, Geumcheon-Gu, Seoul, 08594 Republic of Korea
- 2Department of Food Science and Nutrition, Convergence Program of Material Science for Medicine and Pharmaceutics, Hallym University, Chuncheon, 24252 Republic of Korea
- 3Krefting Research Centre, The Sahlgrenska Academy, University of Gothenburg, Box 424, 405 30 Gothenburg, Sweden
Abstract
Background: Mesenchymal stromal cell-derived exosomes (MSC-EXOs), also called Extracellular Vesicles (EVs), exhibit anti-inflammatory effects in various diseases and are being developed for clinical use. For instance, MSC-EXO promotes skin healing post-laser therapy and improves outcomes in severe COVID-19. Selecting an optimal cellular source is critical for the therapeutic development of MSC-EXO. It has been suggested that GMP-produced MSC-exosomes have slightly different molecular content. This study therefore aimed to compare adipose tissue-derived MSC-EXO (ASC-EXO) from three healthy donors, isolated and characterized under GMP conditions.
Methods: Exosomes were analyzed by nano-tracking analysis (NTA), cryo-electron microscopy, tetraspanin profiling, and multi-omics (proteomics, lipidomics, small RNA sequencing), as well as bioinformatics. Functional assays quantified anti-inflammatory effects in RAW264.7 macrophages and collagen production by human dermal fibroblasts (HDF). In vivo efficacy of ASC-EXOs was evaluated in a house dust mite antigen-induced atopic dermatitis mouse model through histopathological evaluation of ear thickness and differential cell counting.
Results: No significant batch differences were observed in ASC-EXO yield or characteristics. Omics analyses revealed minor variations in protein, lipid, and small RNA cargo among the three batches, but GO-term bioinformatics indicated highly similar functional profiles. IL-6 suppression in RAW264.7 cells and cell proliferation and collagen production by HDF were similar among the ASC-EXO batches. CD73 enzymatic activity was consistent among batches. In vivo, the ASC-EXOs reduced skin thickness and eosinophilia in an atopic dermatitis model, with similar effects among the batches.
Conclusions: In summary, ASC-EXOs from all batches demonstrated comparable anti-inflammatory and collagen-modulating effects in vitro, and similar inhibition of atopic dermatitis signs in vivo. We suggest that biological efficacy combined with bioinformatics analysis should guide MSC-EXO therapeutic quality control assays. These findings support the development of ASC-EXOs for clinical applications.
Supplementary Information: The online version contains supplementary material available at https://doi.org/10.1186/s13287-026-05104-3.
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BibTeX
@article{cho2026consistent,
title = {{Consistent functional properties of MSC-exosomes from different batches revealed by comparative multi-omics, bioinformatics and functional tests}},
author = {Byong Seung Cho and Jun Ho Lee and Kyong-Oh Shin and Kyungho Park and Kyojin Lee and Ella Shin and Hyunju Lee and Healim Lee and Kyong-Su Park and Dae Hyun Ha and Jan Lötvall},
journal = {Stem Cell Research \& Therapy},
year = {2026},
month = jul,
doi = {10.1186/s13287-026-05104-3},
url = {https://doi.org/10.1186/s13287-026-05104-3},
note = {CC BY-NC-ND 4.0},
}
RIS
TY - JOUR
TI - Consistent functional properties of MSC-exosomes from different batches revealed by comparative multi-omics, bioinformatics and functional tests
AU - Byong Seung Cho
AU - Jun Ho Lee
AU - Kyong-Oh Shin
AU - Kyungho Park
AU - Kyojin Lee
AU - Ella Shin
AU - Hyunju Lee
AU - Healim Lee
AU - Kyong-Su Park
AU - Dae Hyun Ha
AU - Jan Lötvall
PY - 2026
DA - 2026/07/21
JO - Stem Cell Research & Therapy
DO - 10.1186/s13287-026-05104-3
UR - https://doi.org/10.1186/s13287-026-05104-3
AB - Background: Mesenchymal stromal cell-derived exosomes (MSC-EXOs), also called Extracellular Vesicles (EVs), exhibit anti-inflammatory effects in various diseases and are being developed for clinical use. For instance, MSC-EXO promotes skin healing post-laser therapy and improves outcomes in severe COVID-19. Selecting an optimal cellular source is critical for the therapeutic development of MSC-EXO. It has been suggested that GMP-produced MSC-exosomes have slightly different molecular content. This study therefore aimed to compare adipose tissue-derived MSC-EXO (ASC-EXO) from three healthy donors, isolated and characterized under GMP conditions. Methods: Exosomes were analyzed by nano-tracking analysis (NTA), cryo-electron microscopy, tetraspanin profiling, and multi-omics (proteomics, lipidomics, small RNA sequencing), as well as bioinformatics. Functional assays quantified anti-inflammatory effects in RAW264.7 macrophages and collagen production by human dermal fibroblasts (HDF). In vivo efficacy of ASC-EXOs was evaluated in a house dust mite antigen-induced atopic dermatitis mouse model through histopathological evaluation of ear thickness and differential cell counting. Results: No significant batch differences were observed in ASC-EXO yield or characteristics. Omics analyses revealed minor variations in protein, lipid, and small RNA cargo among the three batches, but GO-term bioinformatics indicated highly similar functional profiles. IL-6 suppression in RAW264.7 cells and cell proliferation and collagen production by HDF were similar among the ASC-EXO batches. CD73 enzymatic activity was consistent among batches. In vivo, the ASC-EXOs reduced skin thickness and eosinophilia in an atopic dermatitis model, with similar effects among the batches. Conclusions: In summary, ASC-EXOs from all batches demonstrated comparable anti-inflammatory and collagen-modulating effects in vitro, and similar inhibition of atopic dermatitis signs in vivo. We suggest that biological efficacy combined with bioinformatics analysis should guide MSC-EXO therapeutic quality control assays. These findings support the development of ASC-EXOs for clinical applications. Supplementary Information: The online version contains supplementary material available at https://doi.org/10.1186/s13287-026-05104-3.
SN - 1757-6512
ER -