Skip to content
DraconiticResearch
Search Sources API
181,716 studies31 sourcesUpdated 18 min ago
Peer-reviewedCC BY-NC-NDGenome biology

Profiling protein post-translational modifications in plasma-derived extracellular vesicles as fingerprints for breast cancer subtypes.

  • Marco Hadisurya1
  • Hillary Andaluz Aguilar2
  • I-Hsuan Chen3
  • Mengting Xu4
  • Juan Sebastian Paez3,5
  • Rachit Bisht4
  • Jyoti Singh3,6
  • Zheng-Chi Lee3,7
  • Amirhesam Mashaollahi3
  • Anton Iliuk8
  • Weizhou Zhang9
  • W Andy Tao10,11,12,13,14
14 affiliations
  1. 1Department of Biochemistry, Purdue University, West Lafayette, IN, 47907, USA. [email protected].
  2. 2Department of Chemistry, Purdue University, West Lafayette, IN, 47907, USA.
  3. 3Department of Biochemistry, Purdue University, West Lafayette, IN, 47907, USA.
  4. 4School of Electrical and Computer Engineering, Purdue University, West Lafayette, IN, 47907, USA.
  5. 5Borch Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN, 47907, USA.
  6. 6Department of Biochemistry, The Maharaja Sayajirao University of Baroda, Gujarat, India, 390002.
  7. 7Department of Biochemistry, School of Arts and Sciences, University of Pennsylvania, Philadelphia, PA, 19104, USA.
  8. 8Tymora Analytical Operations, West Lafayette, IN, 47906, USA.
  9. 9Department of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL, 32610, USA.
  10. 10Department of Biochemistry, Purdue University, West Lafayette, IN, 47907, USA. [email protected].
  11. 11Department of Chemistry, Purdue University, West Lafayette, IN, 47907, USA. [email protected].
  12. 12Borch Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN, 47907, USA. [email protected].
  13. 13Tymora Analytical Operations, West Lafayette, IN, 47906, USA. [email protected].
  14. 14Purdue Institute for Cancer Research, Purdue University, West Lafayette, IN, 47907, USA. [email protected].
Read full textPDFDOICite

Abstract

Background

Addressing tumor heterogeneity in breast cancer research is crucial, given the distinct subtypes like triple-negative, luminal A/B, and HER2, requiring precise differentiation for effective treatment. This study introduces a non-invasive method by analyzing post-translationally modified proteins in plasma extracellular vesicles, which play a role in immune regulation and intercellular communication. Examining modifications like phosphorylation, acetylation and glycosylation in extracellular vesicles provides insights into breast cancer dynamics. One hundred and one plasma samples from luminal A/B breast cancer, triple-negative breast cancer and healthy individuals underwent discovery and validation experiments.Result

The study identified over 28,000 unique non-modified peptides, 5,000 phosphopeptides, 680 acetyl peptides and 1,300 glycopeptides that are successfully characterized. Bioinformatics analyses revealed significant overexpression of 815 non-modified proteins, 3,958 phosphopeptides, 352 acetyl peptides and 895 glycopeptides in luminal A/B breast cancer or triple-negative breast cancer subtypes. Phosphorylated and glycosylated PD-L1 peptides emerge as potential markers for breast cancer, regardless of subtype. Aligning the findings with literature and PAM50 gene signatures highlights markers correlated with lower survival rates. The study also reports 123 scheduled parallel reaction monitoring analyses, leveraging machine learning to pinpoint a panel of specific modification sites with high accuracy for subtype differentiation.Conclusions

This research reveals diagnostic markers and enhances understanding of the molecular landscape, contributing to more effective and personalized breast cancer diagnostics and treatments.

Related

  1. Novel modifications of small extracellular vesicles to enhance cardiac-specific delivery of siPKA RNA and edaravone against ischemia-reperfusion cardiac injury
    Xing Pei, Rui Li, Yingyu Bai +9
    Materials Today BioPeer-reviewedCC BY-NC-NDFull textMolecular biology & gene regulationDOAJEurope PMCPMC
  2. Biomedical properties and emerging translational potential of bovine milk extracellular vesicles.
    Xiaoqi Guan, Xinxin Hou, Wanting Meng +1
    Discover nanoPeer-reviewedCC BY-NC-NDFull textMolecular biology & gene regulationEurope PMC
  3. Comprehensive multi-post-translational modifications profiling reveals age-associated remodeling in skeletal muscle
    Jian Li, Xin-Qiang Lan, Cai-Fen Guo +7
    Zoological ResearchPeer-reviewedCC BY-NC 4.0Full textMolecular biology & gene regulationPMC
  4. From mucosal activity to medicinal products: translational evidence for Akkermansia muciniphila extracellular vesicles
    Quanli Zhu, Yingchao Liu, Yu Song
    Frontiers in ImmunologyPeer-reviewedCC BY 4.0Full textMolecular biology & gene regulationCrossref
  5. A Hydrodynamic Bioreactor for High-Yield Production of Extracellular Vesicles from Stem Cell Spheroids with Defined Cargo Profiling
    Solène Lenoir, Elliot Thouvenot, Giacomo Gropplero +5
    Advanced SciencePeer-reviewedCC BY 4.0Full text4 citedMolecular biology & gene regulationHAL

Cite

BibTeXRIS
BibTeX
@article{hadisurya2026profiling,
  title = {{Profiling protein post-translational modifications in plasma-derived extracellular vesicles as fingerprints for breast cancer subtypes.}},
  author = {Marco Hadisurya and Hillary Andaluz Aguilar and I-Hsuan Chen and Mengting Xu and Juan Sebastian Paez and Rachit Bisht and Jyoti Singh and Zheng-Chi Lee and Amirhesam Mashaollahi and Anton Iliuk and Weizhou Zhang and W Andy Tao},
  journal = {Genome biology},
  year = {2026},
  month = jul,
  doi = {10.1186/s13059-026-04212-w},
  url = {https://europepmc.org/article/MED/42902395},
  note = {CC BY-NC-ND},
}
RIS
TY  - JOUR
TI  - Profiling protein post-translational modifications in plasma-derived extracellular vesicles as fingerprints for breast cancer subtypes.
AU  - Marco Hadisurya
AU  - Hillary Andaluz Aguilar
AU  - I-Hsuan Chen
AU  - Mengting Xu
AU  - Juan Sebastian Paez
AU  - Rachit Bisht
AU  - Jyoti Singh
AU  - Zheng-Chi Lee
AU  - Amirhesam Mashaollahi
AU  - Anton Iliuk
AU  - Weizhou Zhang
AU  - W Andy Tao
PY  - 2026
DA  - 2026/07/01
JO  - Genome biology
DO  - 10.1186/s13059-026-04212-w
UR  - https://europepmc.org/article/MED/42902395
AB  - Background  Addressing tumor heterogeneity in breast cancer research is crucial, given the distinct subtypes like triple-negative, luminal A/B, and HER2, requiring precise differentiation for effective treatment. This study introduces a non-invasive method by analyzing post-translationally modified proteins in plasma extracellular vesicles, which play a role in immune regulation and intercellular communication. Examining modifications like phosphorylation, acetylation and glycosylation in extracellular vesicles provides insights into breast cancer dynamics. One hundred and one plasma samples from luminal A/B breast cancer, triple-negative breast cancer and healthy individuals underwent discovery and validation experiments.Result  The study identified over 28,000 unique non-modified peptides, 5,000 phosphopeptides, 680 acetyl peptides and 1,300 glycopeptides that are successfully characterized. Bioinformatics analyses revealed significant overexpression of 815 non-modified proteins, 3,958 phosphopeptides, 352 acetyl peptides and 895 glycopeptides in luminal A/B breast cancer or triple-negative breast cancer subtypes. Phosphorylated and glycosylated PD-L1 peptides emerge as potential markers for breast cancer, regardless of subtype. Aligning the findings with literature and PAM50 gene signatures highlights markers correlated with lower survival rates. The study also reports 123 scheduled parallel reaction monitoring analyses, leveraging machine learning to pinpoint a panel of specific modification sites with high accuracy for subtype differentiation.Conclusions  This research reveals diagnostic markers and enhances understanding of the molecular landscape, contributing to more effective and personalized breast cancer diagnostics and treatments.
SN  - 1474-7596
SN  - 1474-760X
ER  -