Characteristics of Staphylococcus lugdunensis isolated from humans and animals
- 1Department of Epizootiology and Clinic of Birds and Exotic Animals, Division of Infectious Diseases and Veterinary Sciences, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences
Abstract
Staphylococcus lugdunensis is an opportunistic coagulase-negative Staphylococcus increasingly reported in both humans and companion animals. In this study, we performed a comprehensive characterization of S. lugdunensis isolates obtained from different hosts and clinical backgrounds. Species identification was conducted using MALDI-TOF MS and confirmed by PCR targeting the species-specific fbl gene, complemented by partial rpoB sequencing. The isolates were analysed using multilocus sequence typing (MLST), PCR-based detection of antimicrobial resistance genes, and phenotypic antimicrobial susceptibility testing. Biofilm formation was assessed using a crystal violet microtiter plate assay under different incubation temperatures, and the virulence of selected strains was evaluated using the Galleria mellonella larvae infection model. The isolates exhibited genetic diversity and variable antimicrobial resistance and biofilm phenotypes. Among the analysed isolates, biofilm production was significantly influenced by incubation temperature and host origin, and selected strains caused differential larval survival in the G. mellonella model. Collectively, these findings highlight the heterogeneity of the analysed S. lugdunensis collection comprising human- and animal-derived isolates and support the need for further studies within the One Health framework.
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BibTeX
@article{prorok2026characteristics,
title = {{Characteristics of Staphylococcus lugdunensis isolated from humans and animals}},
author = {Paulina Prorok and Milena Skrok and Magdalena Karwańska and Magdalena Siedlecka and Marta Miszczak and Krzysztof Rypuła and Karolina Bierowiec},
journal = {Scientific Reports},
year = {2026},
month = jul,
doi = {10.1038/s41598-026-62992-4},
url = {https://doi.org/10.1038/s41598-026-62992-4},
note = {CC BY},
}
RIS
TY - JOUR
TI - Characteristics of Staphylococcus lugdunensis isolated from humans and animals
AU - Paulina Prorok
AU - Milena Skrok
AU - Magdalena Karwańska
AU - Magdalena Siedlecka
AU - Marta Miszczak
AU - Krzysztof Rypuła
AU - Karolina Bierowiec
PY - 2026
DA - 2026/07/01
JO - Scientific Reports
DO - 10.1038/s41598-026-62992-4
UR - https://doi.org/10.1038/s41598-026-62992-4
AB - Staphylococcus lugdunensis is an opportunistic coagulase-negative Staphylococcus increasingly reported in both humans and companion animals. In this study, we performed a comprehensive characterization of S. lugdunensis isolates obtained from different hosts and clinical backgrounds. Species identification was conducted using MALDI-TOF MS and confirmed by PCR targeting the species-specific fbl gene, complemented by partial rpoB sequencing. The isolates were analysed using multilocus sequence typing (MLST), PCR-based detection of antimicrobial resistance genes, and phenotypic antimicrobial susceptibility testing. Biofilm formation was assessed using a crystal violet microtiter plate assay under different incubation temperatures, and the virulence of selected strains was evaluated using the Galleria mellonella larvae infection model. The isolates exhibited genetic diversity and variable antimicrobial resistance and biofilm phenotypes. Among the analysed isolates, biofilm production was significantly influenced by incubation temperature and host origin, and selected strains caused differential larval survival in the G. mellonella model. Collectively, these findings highlight the heterogeneity of the analysed S. lugdunensis collection comprising human- and animal-derived isolates and support the need for further studies within the One Health framework.
SN - 2045-2322
ER -