Prevalence and risk factors for persistent faecal carriage of extended spectrum beta-lactamase producing Escherichia coli in a paediatric community population
Abstract
OBJECTIVE: To investigate clinical and microbiological factors associated with persistent faecal carriage of extended spectrum beta-lactamase (ESBL) producing Escherichia coli in infants. METHODS: Between 2010 and 2022, children aged 3 months to 2 years old were sampled in a community setting in France, at two visits, V1 and V2, 3-24 months apart, to screen for prolonged faecal carriage of ESBL-producing E. coli. Patient clinical information and whole genome sequence of each isolate were used for association studies. RESULTS: A total of 4641 children were sampled. 375 (8%) carried an ESBL-producing Enterobacterales, among which 142 of ESBL-producing E. coli carriers were once again sampled at V2 and included in this study. 21.8% (n = 31/142) and 18.4% (n = 16/99) carried the same ESBL-producing E. coli clone for at least 3 and 6 months, respectively. B2 phylogroup, and among which ST131 clones were associated with an increased risk of persistent carriage. Multivariate analysis identified virulence associated genes involved in adhesion (papC/papGII allele and a tia-like gene) and encoding toxin (senB) as major risk factors for persistence. A genome wide association study highlighted the potential role of the frz metabolic operon, known to be involved in enterocytes adhesion/internalisation. CONCLUSION: Main extraintestinal pathogenic E. coli genomic features (phylogenetic background, adhesion properties) are associated with ESBL-producing E. coli gut colonisation persistence in infants, which could potentially lead to an increased risk of febrile urinary tract infection in these patients.
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BibTeX
@article{lignieres2026prevalence,
title = {{Prevalence and risk factors for persistent faecal carriage of extended spectrum beta-lactamase producing Escherichia coli in a paediatric community population}},
author = {Gabriel Lignieres and Philippe Bidet and André Birgy and Kevin La and Guillaume Geslain and Erick Denamur and Stéphane Béchet and Olivier Cotto and François Blanquart and Franck Thollot and François Corrard and Céline Courroux and Elsa Sobral and Robert Cohen and Corinne Levy and Stéphane Bonacorsi},
journal = {International Journal of Medical Microbiology},
year = {2026},
month = sep,
doi = {10.1016/j.ijmm.2026.151734},
url = {https://hal.science/hal-05751934v1},
note = {CC BY-NC},
}
RIS
TY - JOUR
TI - Prevalence and risk factors for persistent faecal carriage of extended spectrum beta-lactamase producing Escherichia coli in a paediatric community population
AU - Gabriel Lignieres
AU - Philippe Bidet
AU - André Birgy
AU - Kevin La
AU - Guillaume Geslain
AU - Erick Denamur
AU - Stéphane Béchet
AU - Olivier Cotto
AU - François Blanquart
AU - Franck Thollot
AU - François Corrard
AU - Céline Courroux
AU - Elsa Sobral
AU - Robert Cohen
AU - Corinne Levy
AU - Stéphane Bonacorsi
PY - 2026
DA - 2026/09/16
JO - International Journal of Medical Microbiology
DO - 10.1016/j.ijmm.2026.151734
UR - https://hal.science/hal-05751934v1
AB - OBJECTIVE: To investigate clinical and microbiological factors associated with persistent faecal carriage of extended spectrum beta-lactamase (ESBL) producing Escherichia coli in infants. METHODS: Between 2010 and 2022, children aged 3 months to 2 years old were sampled in a community setting in France, at two visits, V1 and V2, 3-24 months apart, to screen for prolonged faecal carriage of ESBL-producing E. coli. Patient clinical information and whole genome sequence of each isolate were used for association studies. RESULTS: A total of 4641 children were sampled. 375 (8%) carried an ESBL-producing Enterobacterales, among which 142 of ESBL-producing E. coli carriers were once again sampled at V2 and included in this study. 21.8% (n = 31/142) and 18.4% (n = 16/99) carried the same ESBL-producing E. coli clone for at least 3 and 6 months, respectively. B2 phylogroup, and among which ST131 clones were associated with an increased risk of persistent carriage. Multivariate analysis identified virulence associated genes involved in adhesion (papC/papGII allele and a tia-like gene) and encoding toxin (senB) as major risk factors for persistence. A genome wide association study highlighted the potential role of the frz metabolic operon, known to be involved in enterocytes adhesion/internalisation. CONCLUSION: Main extraintestinal pathogenic E. coli genomic features (phylogenetic background, adhesion properties) are associated with ESBL-producing E. coli gut colonisation persistence in infants, which could potentially lead to an increased risk of febrile urinary tract infection in these patients.
SN - 1438-4221
SN - 1618-0607
ER -