Staphylococcus aureus derived extracellular vesicles modulate osteoblast-like cell immune responses independently of vesicle internalization
Abstract
Staphylococcus aureus is a major opportunistic Gram-positive pathogen whose virulence is partly mediated by extracellular vesicles (EVs). Here, we examined the production, composition, and functionality of EVs released by the methicillin-resistant strain N315 under infection-mimicking conditions, focusing on their interaction with MG-63 human osteoblast-like cells. N315 EVs, purified by size exclusion chromatography, and characterized by nanoparticle tracking analysis and electron microscopy, contained a selectively enriched and diverse molecular cargo, including proteins, lipoproteins, DNA, RNA, lipoteichoic acid, and peptidoglycan. Confocal microscopy and protection assays confirmed the intravesicular localization of RNAs and proteins. EVs were recognized by TLR2 on MG-63 cells and selectively induced the expression of genes involved in Toll-like receptor, NF-κB, JAK/STAT, and inflammasome pathways. The induction of these pathways was confirmed at the protein level, as well as by increased IL-8 release and caspase-1 activation. EVs were internalized via a dynamin-dependent endocytosis mechanism and trafficked to late endosomes. Importantly, inhibition of uptake did not affect the expression of target genes, demonstrating an uncoupling between EVs internalization and transcriptional response. This work provides new insight into how S. aureus N315 EVs interact with osteoblast-like cells and modulate host responses, revealing both conserved mechanisms and strain- or cell type-specific differences.
Related
- Surface curvature promotes coordinated extracellular matrix remodeling and mechanotransductive signaling during osteoblast adaptation
- Cross-regulatory sRNA networks shape adaptive stress responses in Staphylococcus aureus: RsaC and RsaOI regulate galactose metabolism and cell wall homeostasis
- Corneal epithelial signals remodel bacterial physiology and extracellular vesicle cargo in Pseudomonas aeruginosa and Staphylococcus epidermidis
- Gonadal and chromosomal sex independently and synergistically modulate innate and adaptive immune responses to implanted scaffolds
- House Dust Mite Induces Distinct Cellular and Extracellular Vesicle-Associated miRNA Signatures in BEAS-2B Cells: Targeted Analysis of IL-4 Responses
BibTeX
@article{papail2026staphylococcus,
title = {{Staphylococcus aureus derived extracellular vesicles modulate osteoblast-like cell immune responses independently of vesicle internalization}},
author = {Julia Papail and Nathalie Daniel and Ligia Prado and Danièle Vassaux and Sandrine Péron and Céline Even and Véronique Lebret and Brenda Silva Rosa da Luz and Yann Le Gouar and Julien Jardin and Nadia Berkova and Siomar de Castro Soares and Kenichi Yoshida and Vasco Azevedo and Svetlana Chabelskaya and Yves Le Loir and Eric Guédon},
journal = {Scientific Reports},
year = {2026},
month = oct,
doi = {10.1038/s41598-026-54792-7},
url = {https://hal.science/hal-05649734v1},
note = {CC BY-NC-ND 4.0},
}
RIS
TY - JOUR
TI - Staphylococcus aureus derived extracellular vesicles modulate osteoblast-like cell immune responses independently of vesicle internalization
AU - Julia Papail
AU - Nathalie Daniel
AU - Ligia Prado
AU - Danièle Vassaux
AU - Sandrine Péron
AU - Céline Even
AU - Véronique Lebret
AU - Brenda Silva Rosa da Luz
AU - Yann Le Gouar
AU - Julien Jardin
AU - Nadia Berkova
AU - Siomar de Castro Soares
AU - Kenichi Yoshida
AU - Vasco Azevedo
AU - Svetlana Chabelskaya
AU - Yves Le Loir
AU - Eric Guédon
PY - 2026
DA - 2026/10/13
JO - Scientific Reports
DO - 10.1038/s41598-026-54792-7
UR - https://hal.science/hal-05649734v1
AB - Staphylococcus aureus is a major opportunistic Gram-positive pathogen whose virulence is partly mediated by extracellular vesicles (EVs). Here, we examined the production, composition, and functionality of EVs released by the methicillin-resistant strain N315 under infection-mimicking conditions, focusing on their interaction with MG-63 human osteoblast-like cells. N315 EVs, purified by size exclusion chromatography, and characterized by nanoparticle tracking analysis and electron microscopy, contained a selectively enriched and diverse molecular cargo, including proteins, lipoproteins, DNA, RNA, lipoteichoic acid, and peptidoglycan. Confocal microscopy and protection assays confirmed the intravesicular localization of RNAs and proteins. EVs were recognized by TLR2 on MG-63 cells and selectively induced the expression of genes involved in Toll-like receptor, NF-κB, JAK/STAT, and inflammasome pathways. The induction of these pathways was confirmed at the protein level, as well as by increased IL-8 release and caspase-1 activation. EVs were internalized via a dynamin-dependent endocytosis mechanism and trafficked to late endosomes. Importantly, inhibition of uptake did not affect the expression of target genes, demonstrating an uncoupling between EVs internalization and transcriptional response. This work provides new insight into how S. aureus N315 EVs interact with osteoblast-like cells and modulate host responses, revealing both conserved mechanisms and strain- or cell type-specific differences.
SN - 2045-2322
ER -