Molecular Mechanisms of the Anticancer Activity of Hinokitiol in C32 Melanoma Cells: Transcriptomic Analysis and Modulation by a Static Magnetic Field
- 1Department of Nutrigenomics and Bromatology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, Katowice, 8 Jednosci Street, 41-200 Sosnowiec, Poland
- 2Department of Molecular Biology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, Katowice, 8 Jednosci Street, 41-200 Sosnowiec, Poland
Abstract
Many studies confirm that natural substances derived from plant materials, at appropriate concentrations, can exhibit anticancer effects against various types of cancer cells. One such substance is hinokitiol, which has been proven to have anticancer effects against malignant melanoma. However, the molecular mechanisms of this effect are unknown. Therefore, the aim of this study was to determine the probable molecular mechanism of the anticancer effect of hinokitiol through gene expression analysis. Additionally, it was examined whether a ubiquitous static magnetic field could modify the anticancer properties of hinokitiol. Transcriptome analysis was performed using oligonucleotide microarrays, followed by validation of the obtained results for five genes using RT-qPCR. Fifty-six statistically different transcriptomes were found in melanoma cultures treated with only hinokitiol (p < 0.05, FC > 1.5). To confirm the results obtained from the microarray, RT-qPCR was performed for five genes: the ARNTL, CRYAB, MAT2A, HSPA8 and NR1D1 genes. In summary, our studies indicate that the addition of hinokitiol to melanoma cells changes the expression of numerous genes, including those associated with the biological clock. The presented results are preliminary and point out a direction for future research. The presence of a static magnetic field does not cause statistically significant changes in gene expression. Only in ARNTL was a statistically significant decrease in expression levels observed in the H+SMF group compared to the H group.
Related
- Molecular mechanisms involved in the anticancer activities of probiotics and postbiotics.
- Targeting Sirtuins in Neurodegenerative and Psychiatric Disorders: From Molecular Mechanisms to Pharmacological Modulation
- Targeting glial modulation: molecular mechanisms of Polygonatum in neurological disorders
- Transcriptomic analysis identifies candidate molecular mechanisms underlying SCF+G-CSF-reduced neuropathology in aged APP/PS1 mice.
- A whole-blood transcriptomic analysis of shared molecular mechanisms between subclinical atherosclerosis and hypothyroidism in women
BibTeX
@article{synowiecwojtarowicz2026molecular,
title = {{Molecular Mechanisms of the Anticancer Activity of Hinokitiol in C32 Melanoma Cells: Transcriptomic Analysis and Modulation by a Static Magnetic Field}},
author = {Agnieszka Synowiec-Wojtarowicz and Celina Kruszniewska-Rajs and Agata Krawczyk and Magdalena Kimsa-Dudek},
journal = {Molecules},
year = {2026},
month = oct,
doi = {10.3390/molecules31203688},
url = {https://www.mdpi.com/1420-3049/31/20/3688},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Molecular Mechanisms of the Anticancer Activity of Hinokitiol in C32 Melanoma Cells: Transcriptomic Analysis and Modulation by a Static Magnetic Field
AU - Agnieszka Synowiec-Wojtarowicz
AU - Celina Kruszniewska-Rajs
AU - Agata Krawczyk
AU - Magdalena Kimsa-Dudek
PY - 2026
DA - 2026/10/01
JO - Molecules
DO - 10.3390/molecules31203688
UR - https://www.mdpi.com/1420-3049/31/20/3688
AB - Many studies confirm that natural substances derived from plant materials, at appropriate concentrations, can exhibit anticancer effects against various types of cancer cells. One such substance is hinokitiol, which has been proven to have anticancer effects against malignant melanoma. However, the molecular mechanisms of this effect are unknown. Therefore, the aim of this study was to determine the probable molecular mechanism of the anticancer effect of hinokitiol through gene expression analysis. Additionally, it was examined whether a ubiquitous static magnetic field could modify the anticancer properties of hinokitiol. Transcriptome analysis was performed using oligonucleotide microarrays, followed by validation of the obtained results for five genes using RT-qPCR. Fifty-six statistically different transcriptomes were found in melanoma cultures treated with only hinokitiol (p < 0.05, FC > 1.5). To confirm the results obtained from the microarray, RT-qPCR was performed for five genes: the ARNTL, CRYAB, MAT2A, HSPA8 and NR1D1 genes. In summary, our studies indicate that the addition of hinokitiol to melanoma cells changes the expression of numerous genes, including those associated with the biological clock. The presented results are preliminary and point out a direction for future research. The presence of a static magnetic field does not cause statistically significant changes in gene expression. Only in ARNTL was a statistically significant decrease in expression levels observed in the H+SMF group compared to the H group.
SN - 1420-3049
ER -