Cross‐Reactive Neutralizing Antibodies Between ZIKV and DENV May Exhibit Serotype Specificity
4 affiliations
- 1Department of Biosciences and Technology Institute of Tropical Pathology and Public Health Federal University of Goiás Goiânia Goiás Brazil
- 2Innovation Hub in Point of Care Technologies Federal University of Goiás‐Merck S/A Alliance Goiânia Goiás Brazil
- 3Department of Biomedical Engineering Emory University Atlanta Geogia USA
- 4Department of Cell Biology Institute of Biology University of Brasília Brasília Brazil
Abstract
Dengue virus (DENV) and Zika virus (ZIKV) are public health threats, causing substantial morbidity and mortality worldwide. The co‐circulation of DENV and ZIKV complicates prevention and control efforts, highlighting the importance of studying cross‐reactive antibodies, vaccines, and immune modulators. This study explores the cross‐reactivity and neutralizing capacity of antibodies against DENV elicited by immunization with a ZIKV protein extract. Female isogenic BALB/c mice were immunized with ZIKV protein extract derived from C6/36 cell cultures. Splenic B lymphocytes from immunized mice were fused with myeloma cells using polyethylene glycol (PEG) to generate hybridomas. The resulting antibodies were assessed using ELISA and plaque reduction neutralization tests (PRNT) against ZIKV and DENV including their structural and nonstructural proteins. Our findings showed that immunization induced a diverse antibody response, including antibodies recognizing epitopes unique to ZIKV as well as epitopes shared with DENV. Notably, neutralizing antibodies targeting conserved epitopes, particularly those shared with DENV‐1, were also identified against other DENV serotypes. These results suggest the presence of cross‐reactive neutralizing antibody responses between ZIKV and DENV, potentially exhibiting serotype‐specific reactivity. Collectively, this study provides experimental evidence of flavivirus cross‐reactivity and contributes to a more refined understanding of humoral responses in settings where these viruses co‐circulate.
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BibTeX
@article{silveira2026crossreactive,
title = {{Cross‐Reactive Neutralizing Antibodies Between ZIKV and DENV May Exhibit Serotype Specificity}},
author = {Lucimeire Antonelli da Silveira and Djairo Pastor Saavedra and Isabela Cinquini Junqueira and Jefferson do Carmo Dietz and Cristina Rodrigues de Oliveira and Leonardo Lopes‐Luz and Lorena Carvalho de Souza Chaves and Bergmann Morais Ribeiro},
journal = {Biotechnology Journal},
year = {2026},
month = oct,
doi = {10.1002/biot.70323},
url = {https://doi.org/10.1002/biot.70323},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Cross‐Reactive Neutralizing Antibodies Between ZIKV and DENV May Exhibit Serotype Specificity
AU - Lucimeire Antonelli da Silveira
AU - Djairo Pastor Saavedra
AU - Isabela Cinquini Junqueira
AU - Jefferson do Carmo Dietz
AU - Cristina Rodrigues de Oliveira
AU - Leonardo Lopes‐Luz
AU - Lorena Carvalho de Souza Chaves
AU - Bergmann Morais Ribeiro
PY - 2026
DA - 2026/10/01
JO - Biotechnology Journal
DO - 10.1002/biot.70323
UR - https://doi.org/10.1002/biot.70323
AB - Dengue virus (DENV) and Zika virus (ZIKV) are public health threats, causing substantial morbidity and mortality worldwide. The co‐circulation of DENV and ZIKV complicates prevention and control efforts, highlighting the importance of studying cross‐reactive antibodies, vaccines, and immune modulators. This study explores the cross‐reactivity and neutralizing capacity of antibodies against DENV elicited by immunization with a ZIKV protein extract. Female isogenic BALB/c mice were immunized with ZIKV protein extract derived from C6/36 cell cultures. Splenic B lymphocytes from immunized mice were fused with myeloma cells using polyethylene glycol (PEG) to generate hybridomas. The resulting antibodies were assessed using ELISA and plaque reduction neutralization tests (PRNT) against ZIKV and DENV including their structural and nonstructural proteins. Our findings showed that immunization induced a diverse antibody response, including antibodies recognizing epitopes unique to ZIKV as well as epitopes shared with DENV. Notably, neutralizing antibodies targeting conserved epitopes, particularly those shared with DENV‐1, were also identified against other DENV serotypes. These results suggest the presence of cross‐reactive neutralizing antibody responses between ZIKV and DENV, potentially exhibiting serotype‐specific reactivity. Collectively, this study provides experimental evidence of flavivirus cross‐reactivity and contributes to a more refined understanding of humoral responses in settings where these viruses co‐circulate.
SN - 1860-6768
SN - 1860-7314
ER -