Constructing Virtual Viruses in A Spatial Minimal Cell
- 1Army Medical University
Abstract
Viral development links gene expression and genome replication to particle formation within a cell. Here we construct virtual viruses in a JCVI-syn3A-based spatial minimal cell. For mycoplasma virus P1, molecular events connect expression and replication to coarse-grained packaging, followed by a spatial reconstruction of membrane opening and particle release. Individual finite-size particles coexist, move across the boundary and undergo partial crossings and returns before complete exit. In contrast, Acholeplasma phage MV-L1 (L1) expresses its genes and accumulates genomes as the host continues to grow and develops a constricted shape. The coexistence of viral activity and continued host growth is qualitatively consistent with the non-lytic biology of group 1 acholeplasmaviruses. These reconstructions extend the virtual cell to the viruses that develop within it, linking molecular activity, particle behavior and host growth.
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BibTeX
@misc{sun2026constructing,
title = {{Constructing Virtual Viruses in A Spatial Minimal Cell}},
author = {W. Sun and Y. Tang and M. Qiu and J. Li and F. Luo and C. Dou},
howpublished = {bioRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.22.753465},
url = {https://www.biorxiv.org/content/10.64898/2026.09.22.753465v1},
note = {CC BY-NC-ND},
}
RIS
TY - UNPB
TI - Constructing Virtual Viruses in A Spatial Minimal Cell
AU - W. Sun
AU - Y. Tang
AU - M. Qiu
AU - J. Li
AU - F. Luo
AU - C. Dou
PY - 2026
DA - 2026/09/22
PB - bioRxiv
DO - 10.64898/2026.09.22.753465
UR - https://www.biorxiv.org/content/10.64898/2026.09.22.753465v1
AB - Viral development links gene expression and genome replication to particle formation within a cell. Here we construct virtual viruses in a JCVI-syn3A-based spatial minimal cell. For mycoplasma virus P1, molecular events connect expression and replication to coarse-grained packaging, followed by a spatial reconstruction of membrane opening and particle release. Individual finite-size particles coexist, move across the boundary and undergo partial crossings and returns before complete exit. In contrast, Acholeplasma phage MV-L1 (L1) expresses its genes and accumulates genomes as the host continues to grow and develops a constricted shape. The coexistence of viral activity and continued host growth is qualitatively consistent with the non-lytic biology of group 1 acholeplasmaviruses. These reconstructions extend the virtual cell to the viruses that develop within it, linking molecular activity, particle behavior and host growth.
ER -