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Peer-reviewedCC BYMicrobiology (Reading, England)

Proteomics insight into phosphate limitation response in Burkholderia phytopathogens.

  • Lixian Xu1
  • Manpreet Kaur1,2
  • Jong Hyun Ham3
  • Mengcen Wang4
  • Robert W Jackson1,2
  • Yin Chen1,2
4 affiliations
  1. 1School of Biosciences, University of Birmingham, Birmingham, B15 2TT, UK.
  2. 2Institute of Microbiology and Infection, University of Birmingham, Birmingham B15 2TT, UK.
  3. 3Department of Plant Pathology and Crop Physiology, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA.
  4. 4College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang 310058, PR China.
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Abstract

Inorganic phosphate (Pi) limitation is a pervasive environmental constraint that shapes microbial behaviour in soil and plant-associated niches. To determine how Pi scarcity influences the pathogenic potential of rice-infecting Burkholderia species, we performed comparative proteomic and lipidomic analyses of Burkholderia glumae and Burkholderia plantarii grown under Pi-replete and Pi-deplete conditions. Pi starvation triggered strong activation of the Pho regulon in both organisms, including induction of alkaline phosphatases, phosphonate transporters and the high-affinity PstSABC uptake system. Beyond classical phosphate-stress responses, Pi limitation markedly enriched proteins associated with virulence, secretion systems, toxins, chemotaxis and cell envelope modification. Both species also activated PlcP/Agt-mediated lipid remodelling pathways and synthesized phosphorus-free glycolipids exclusively during Pi depletion, indicating substantial membrane lipid restructuring. These results demonstrate that Pi limitation acts as a major environmental cue coordinating nutrient acquisition with multiple virulence-related functions. Our findings provide new insight into how phosphate scarcity shapes the environmental fitness and pathogenic capacity of Burkholderia phytopathogens and highlight nutrient limitation as an important driver of plant disease emergence.

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Cite

BibTeXRIS
BibTeX
@article{xu2026proteomics,
  title = {{Proteomics insight into phosphate limitation response in Burkholderia phytopathogens.}},
  author = {Lixian Xu and Manpreet Kaur and Jong Hyun Ham and Mengcen Wang and Robert W Jackson and Yin Chen},
  journal = {Microbiology (Reading, England)},
  year = {2026},
  month = oct,
  doi = {10.1099/mic.0.001758},
  url = {https://europepmc.org/article/MED/42879194},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Proteomics insight into phosphate limitation response in Burkholderia phytopathogens.
AU  - Lixian Xu
AU  - Manpreet Kaur
AU  - Jong Hyun Ham
AU  - Mengcen Wang
AU  - Robert W Jackson
AU  - Yin Chen
PY  - 2026
DA  - 2026/10/01
JO  - Microbiology (Reading, England)
DO  - 10.1099/mic.0.001758
UR  - https://europepmc.org/article/MED/42879194
AB  - Inorganic phosphate (Pi) limitation is a pervasive environmental constraint that shapes microbial behaviour in soil and plant-associated niches. To determine how Pi scarcity influences the pathogenic potential of rice-infecting Burkholderia species, we performed comparative proteomic and lipidomic analyses of Burkholderia glumae and Burkholderia plantarii grown under Pi-replete and Pi-deplete conditions. Pi starvation triggered strong activation of the Pho regulon in both organisms, including induction of alkaline phosphatases, phosphonate transporters and the high-affinity PstSABC uptake system. Beyond classical phosphate-stress responses, Pi limitation markedly enriched proteins associated with virulence, secretion systems, toxins, chemotaxis and cell envelope modification. Both species also activated PlcP/Agt-mediated lipid remodelling pathways and synthesized phosphorus-free glycolipids exclusively during Pi depletion, indicating substantial membrane lipid restructuring. These results demonstrate that Pi limitation acts as a major environmental cue coordinating nutrient acquisition with multiple virulence-related functions. Our findings provide new insight into how phosphate scarcity shapes the environmental fitness and pathogenic capacity of Burkholderia phytopathogens and highlight nutrient limitation as an important driver of plant disease emergence.
SN  - 1350-0872
SN  - 1465-2080
ER  -