Comparative Evaluation of Physicochemical Stability and In Vitro Intestinal Dialysability of Conventional Solid and Liquid Nanoscale Vitamin Premixes in Swine Feed.
- 1Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
- 2Sanya Institute of Nanjing Agricultural University, Nanjing Agricultural University, Sanya 572025, China.
Abstract
Fat-soluble vitamins (FSVs) are essential micronutrients in swine nutrition, but their stability can be affected by formulation characteristics, storage conditions, thermal processing, and gastrointestinal environments. This study aimed to compare the physicochemical stability and in vitro intestinal dialysability of vitamins A, D, and E between two non-identical commercial formulations: conventional solid vitamin premixes (SVPs) and liquid nanoscale vitamin premixes (LNVPs) under simulated storage, thermal treatment, gastrointestinal digestion, dialysis, and fecal fermentation conditions. Microscopy revealed irregular solid particles in SVPs and spherical nanoscale structures observed in LNVPs under transmission electron microscopy conditions, with the latter showing improved visual dispersion stability. Vitamin retention decreased progressively during storage, and LNVPs maintained significantly higher relative retention of FSVs than SVPs (p < 0.01). After 40 days of storage at 40 °C, LNVPs retained more than 65% of vitamins D and E, whereas SVPs retained less than 50%. Under high-temperature stress simulating feed pelleting, LNVPs also showed higher retention of vitamins D and E than SVPs. In the simulated porcine gastrointestinal digestion model, LNVPs showed lower early apparent disappearance and higher dialysable fractions during the intestinal phase for all three FSVs (p < 0.01). A subsequent fecal fermentation assay further revealed formulation-dependent differences in vitamin behavior under simulated colonic conditions. Collectively, LNVPs showed higher relative vitamin retention and greater in vitro dialysability than SVPs under the tested conditions, supporting the potential of LNVPs for improving vitamin preservation in swine feed applications.
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BibTeX
@article{zhang2026comparative,
title = {{Comparative Evaluation of Physicochemical Stability and In Vitro Intestinal Dialysability of Conventional Solid and Liquid Nanoscale Vitamin Premixes in Swine Feed.}},
author = {Lujun Zhang and Yamei Wang and Ruxue Tang and Mingming Tao and Xipan Hou and Zhifang Chu and Weijiang Zheng and Wen Yao},
journal = {Veterinary sciences},
year = {2026},
month = oct,
doi = {10.3390/vetsci13101043},
url = {https://europepmc.org/article/MED/42944139},
note = {CC BY},
}
RIS
TY - JOUR
TI - Comparative Evaluation of Physicochemical Stability and In Vitro Intestinal Dialysability of Conventional Solid and Liquid Nanoscale Vitamin Premixes in Swine Feed.
AU - Lujun Zhang
AU - Yamei Wang
AU - Ruxue Tang
AU - Mingming Tao
AU - Xipan Hou
AU - Zhifang Chu
AU - Weijiang Zheng
AU - Wen Yao
PY - 2026
DA - 2026/10/03
JO - Veterinary sciences
DO - 10.3390/vetsci13101043
UR - https://europepmc.org/article/MED/42944139
AB - Fat-soluble vitamins (FSVs) are essential micronutrients in swine nutrition, but their stability can be affected by formulation characteristics, storage conditions, thermal processing, and gastrointestinal environments. This study aimed to compare the physicochemical stability and in vitro intestinal dialysability of vitamins A, D, and E between two non-identical commercial formulations: conventional solid vitamin premixes (SVPs) and liquid nanoscale vitamin premixes (LNVPs) under simulated storage, thermal treatment, gastrointestinal digestion, dialysis, and fecal fermentation conditions. Microscopy revealed irregular solid particles in SVPs and spherical nanoscale structures observed in LNVPs under transmission electron microscopy conditions, with the latter showing improved visual dispersion stability. Vitamin retention decreased progressively during storage, and LNVPs maintained significantly higher relative retention of FSVs than SVPs (p < 0.01). After 40 days of storage at 40 °C, LNVPs retained more than 65% of vitamins D and E, whereas SVPs retained less than 50%. Under high-temperature stress simulating feed pelleting, LNVPs also showed higher retention of vitamins D and E than SVPs. In the simulated porcine gastrointestinal digestion model, LNVPs showed lower early apparent disappearance and higher dialysable fractions during the intestinal phase for all three FSVs (p < 0.01). A subsequent fecal fermentation assay further revealed formulation-dependent differences in vitamin behavior under simulated colonic conditions. Collectively, LNVPs showed higher relative vitamin retention and greater in vitro dialysability than SVPs under the tested conditions, supporting the potential of LNVPs for improving vitamin preservation in swine feed applications.
SN - 2306-7381
ER -