Biological Parts in Yeast Synthetic Biology: From Regulatory Elements to Predictive Design Platforms

Abstract

Yeasts, particularly Saccharomyces cerevisiae, are important eukaryotic chassis for synthetic biology because of their tractable genetics, versatile toolkits, and broad utility in metabolic engineering and functional genomics. Progress in this field has been driven by biological parts that enable programmable control of gene expression and cellular behavior. Early efforts focused mainly on promoters, terminators, and other regulatory elements for tuning individual genes. However, as engineering expanded to multigene pathways, genetic circuits, and dynamic regulatory systems, the limits of part-centric design became clear. Part performance is often shaped by genomic context, chromatin state, host physiology, and interactions with other components, which restricts modularity and predictability. In response, yeast synthetic biology is shifting toward integrated design frameworks combining multilayer regulation, standardized assembly, automated experimentation, and computational modeling. This review provides an integrated perspective on the evolution of biological parts across DNA-, RNA-, and protein-level regulation, connecting these advances with assembly frameworks, biofoundries, and machine learning to trace the trajectory from part-centric engineering toward predictive, system-level design in yeast synthetic biology.

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BibTeX RIS

BibTeX

@article{hong2026biological,
  title = {{Biological Parts in Yeast Synthetic Biology: From Regulatory Elements to Predictive Design Platforms}},
  author = {Sujin Hong and Ho Bum Kang and Min-Jun Seong and Eunha Jeon and Sumin Seo and Seung-Gyun Woo and Eun Joong Oh and Young-Kyoung Park and Youngjoon Lee and Dae-Hee Lee},
  journal = {FEMS Yeast Research},
  year = {2026},
  month = sep,
  doi = {10.1093/femsyr/foag048},
  url = {https://hal.inrae.fr/hal-05767672v1},
  note = {CC BY-NC-ND},
}

RIS

TY  - JOUR
TI  - Biological Parts in Yeast Synthetic Biology: From Regulatory Elements to Predictive Design Platforms
AU  - Sujin Hong
AU  - Ho Bum Kang
AU  - Min-Jun Seong
AU  - Eunha Jeon
AU  - Sumin Seo
AU  - Seung-Gyun Woo
AU  - Eun Joong Oh
AU  - Young-Kyoung Park
AU  - Youngjoon Lee
AU  - Dae-Hee Lee
PY  - 2026
DA  - 2026/09/16
JO  - FEMS Yeast Research
DO  - 10.1093/femsyr/foag048
UR  - https://hal.inrae.fr/hal-05767672v1
AB  - Yeasts, particularly Saccharomyces cerevisiae, are important eukaryotic chassis for synthetic biology because of their tractable genetics, versatile toolkits, and broad utility in metabolic engineering and functional genomics. Progress in this field has been driven by biological parts that enable programmable control of gene expression and cellular behavior. Early efforts focused mainly on promoters, terminators, and other regulatory elements for tuning individual genes. However, as engineering expanded to multigene pathways, genetic circuits, and dynamic regulatory systems, the limits of part-centric design became clear. Part performance is often shaped by genomic context, chromatin state, host physiology, and interactions with other components, which restricts modularity and predictability. In response, yeast synthetic biology is shifting toward integrated design frameworks combining multilayer regulation, standardized assembly, automated experimentation, and computational modeling. This review provides an integrated perspective on the evolution of biological parts across DNA-, RNA-, and protein-level regulation, connecting these advances with assembly frameworks, biofoundries, and machine learning to trace the trajectory from part-centric engineering toward predictive, system-level design in yeast synthetic biology.
SN  - 1567-1356
SN  - 1567-1364
ER  -

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