Enzymatic synthesis of structured lipids from sacha inchi (Plukenetia volubilis) oil with capric acid via acidolysis reaction in stirred tank and packed bed mini reactors
Abstract
The present study describes an efficient and sustainable synthesis method for producing functional triglycerides (FTs) by conducting an acidolysis reaction of sacha inchi (Plukenetia volubilis) oil with capric acid (C10), catalyzed by Rhizopus oryzae (RO) lipase immobilized in low-cost corn cob powder, to obtain medium-large-medium chain (MLM) triglycerides. Initially, acidolysis reactions were performed between sacha inchi oil, C10, and the immobilized enzyme in a stirred tank at various temperatures (35 °C, 45 °C, 55 °C, and 59 °C), different molar ratios (1:2, 1:3, 1:4 oil:fatty acid), and various hydrolytic enzyme activities (83.6, 124, and 217 IU/mg). Subsequently, to optimize the degree of incorporation, the best condition from the stirred tank reactions (45 °C, 1:3 M ratio, and 217 U of hydrolytic activity) was selected, considering the highest nutritional quality of the lipid in terms of the migration of the sn2 position, and was tested in a packed bed reactor with a spatial time of 112 min, residence time of 107 min, and a flow rate of 0.5 mL/min. The highest incorporation degree achieved in packed bed reactor was 36% at 45 °C. Afterward, it was developed for the best condition an integrative platform for the recycling of the RO lipase immobilized in low-cost corn cob powder (up to four successive cycles without any make up). Subsequent assays on the functional triglycerides revealed atherogenic and thrombogenicity lipid index values of 0.04 ± 0.10 and 4.15 ± 0.05, respectively, which were lower than those of pure sacha inchi oil, indicating the potential to enhance the nutritional quality of the oil through C10 incorporation.
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BibTeX
@article{caicedopaz2026enzymatic,
title = {{Enzymatic synthesis of structured lipids from sacha inchi (Plukenetia volubilis) oil with capric acid via acidolysis reaction in stirred tank and packed bed mini reactors}},
author = {Angie Vanessa Caicedo-Paz and Cassamo Ussemane Mussagy and Victoria Mesa and Rodney Helder Miotti Junior and Rodrigo Valenzuela and Ariela Veloso de Paula and Julián Paul Martinez-Galan},
journal = {Food Bioscience},
year = {2026},
month = oct,
doi = {10.1016/j.fbio.2024.103769},
url = {https://hal.science/hal-05777985v1},
note = {CC BY-NC},
}
RIS
TY - JOUR
TI - Enzymatic synthesis of structured lipids from sacha inchi (Plukenetia volubilis) oil with capric acid via acidolysis reaction in stirred tank and packed bed mini reactors
AU - Angie Vanessa Caicedo-Paz
AU - Cassamo Ussemane Mussagy
AU - Victoria Mesa
AU - Rodney Helder Miotti Junior
AU - Rodrigo Valenzuela
AU - Ariela Veloso de Paula
AU - Julián Paul Martinez-Galan
PY - 2026
DA - 2026/10/05
JO - Food Bioscience
DO - 10.1016/j.fbio.2024.103769
UR - https://hal.science/hal-05777985v1
AB - The present study describes an efficient and sustainable synthesis method for producing functional triglycerides (FTs) by conducting an acidolysis reaction of sacha inchi (Plukenetia volubilis) oil with capric acid (C10), catalyzed by Rhizopus oryzae (RO) lipase immobilized in low-cost corn cob powder, to obtain medium-large-medium chain (MLM) triglycerides. Initially, acidolysis reactions were performed between sacha inchi oil, C10, and the immobilized enzyme in a stirred tank at various temperatures (35 °C, 45 °C, 55 °C, and 59 °C), different molar ratios (1:2, 1:3, 1:4 oil:fatty acid), and various hydrolytic enzyme activities (83.6, 124, and 217 IU/mg). Subsequently, to optimize the degree of incorporation, the best condition from the stirred tank reactions (45 °C, 1:3 M ratio, and 217 U of hydrolytic activity) was selected, considering the highest nutritional quality of the lipid in terms of the migration of the sn2 position, and was tested in a packed bed reactor with a spatial time of 112 min, residence time of 107 min, and a flow rate of 0.5 mL/min. The highest incorporation degree achieved in packed bed reactor was 36% at 45 °C. Afterward, it was developed for the best condition an integrative platform for the recycling of the RO lipase immobilized in low-cost corn cob powder (up to four successive cycles without any make up). Subsequent assays on the functional triglycerides revealed atherogenic and thrombogenicity lipid index values of 0.04 ± 0.10 and 4.15 ± 0.05, respectively, which were lower than those of pure sacha inchi oil, indicating the potential to enhance the nutritional quality of the oil through C10 incorporation.
SN - 2212-4292
SN - 2212-4306
ER -