Peer-reviewedCC BYMicroPublication biology
A Simple Pluronic F-127 Pretreatment Enables Near-Complete Recovery of C. elegans from Plastic Pipette Tips and Microcentrifuge Tubes.
- 1Biochemistry and Microbiology, Oklahoma State University Center for Health Sciences, Tulsa, OK, United States.
Abstract
Adhesion of Caenorhabditis elegans to plastic pipette tips and microcentrifuge tubes is a common source of sample loss and variability in experimental workflows. Here, we describe a simple consumable-preparation method using 0.5% Pluronic F-127 to reduce worm sticking and improve recovery from plastic surfaces. As a less hazardous alternative to traditional siliconization procedures, Pluronic-treated tips and tubes enabled consistent worm release, while increasing the concentration to 1% provided no additional benefit. Recovery efficiency was not retained following autoclaving of treated consumables. This protocol offers a practical and accessible approach to improving reproducibility in C. elegans handling and sample-processing workflows.
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BibTeX
@article{shults2026simple,
title = {{A Simple Pluronic F-127 Pretreatment Enables Near-Complete Recovery of C. elegans from Plastic Pipette Tips and Microcentrifuge Tubes.}},
author = {Crystal A Shults and Jacob Manjarrez},
journal = {MicroPublication biology},
year = {2026},
month = oct,
doi = {10.17912/micropub.biology.002332},
url = {https://europepmc.org/article/MED/42999579},
note = {CC BY},
}
RIS
TY - JOUR
TI - A Simple Pluronic F-127 Pretreatment Enables Near-Complete Recovery of C. elegans from Plastic Pipette Tips and Microcentrifuge Tubes.
AU - Crystal A Shults
AU - Jacob Manjarrez
PY - 2026
DA - 2026/10/22
JO - MicroPublication biology
DO - 10.17912/micropub.biology.002332
UR - https://europepmc.org/article/MED/42999579
AB - Adhesion of Caenorhabditis elegans to plastic pipette tips and microcentrifuge tubes is a common source of sample loss and variability in experimental workflows. Here, we describe a simple consumable-preparation method using 0.5% Pluronic F-127 to reduce worm sticking and improve recovery from plastic surfaces. As a less hazardous alternative to traditional siliconization procedures, Pluronic-treated tips and tubes enabled consistent worm release, while increasing the concentration to 1% provided no additional benefit. Recovery efficiency was not retained following autoclaving of treated consumables. This protocol offers a practical and accessible approach to improving reproducibility in C. elegans handling and sample-processing workflows.
SN - 2578-9430
ER -