Cellular and Tissue Mechanisms of Laser-Assisted Regeneration of Tympanic Membrane Perforation
5 affiliations
- 1Department of Clinical Pharmacology and Propaedeutics of Internal Diseases, Sechenov First Moscow State Medical University, Moscow 119991, Russia
- 2Engineering Physics Institute for Biomedicine, Moscow Engineering Physics Institute (National Research Nuclear University), Moscow 115409, Russia
- 3Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Laboratory of Pharmacology and Health Technology Assessment, Moscow 117198, Russia
- 4Department of Ear, Nose and Throat Diseases, Sechenov First Moscow State Medical University, Moscow 119991, Russia
- 5National Research Centre “Kurchatov Institute”, Moscow 123182, Russia
Abstract
Chronic tympanic membrane (TM) perforations remain a significant clinical problem due to limited spontaneous healing and the drawbacks of surgical interventions. The present study aimed to evaluate the effects of low-intensity infrared laser irradiation on cellular mechanisms of regeneration and to assess its therapeutic potential in TM repair. Primary fibroblast cultures isolated from chinchilla tissue were exposed to laser radiation at wavelengths 1560 nm and 1450 nm. Cellular responses were analyzed using MTT assay, scratch test, Ki-67 immunostaining, and ELISA for collagen type II. In addition, an in vivo model of chronic TM perforation in chinchillas was used to validate the regenerative effects under physiological conditions. Laser irradiation was associated with accelerated scratch closure and increased extracellular matrix production under selected irradiation conditions, whereas metabolic and proliferative indices decreased over time, most likely reflecting culture confluence and maturation rather than cytotoxicity. In vivo, complete closure of tympanic membrane perforations was observed in all laser-treated ears in the context of collagen sponge-assisted repair, whereas scaffold-only control perforations remained unclosed. These findings support the potential of low-intensity infrared laser irradiation as a minimally invasive adjunct to scaffold-assisted tympanic membrane repair. However, the proposed involvement of endogenous progenitor cell populations should be regarded as a hypothesis requiring further histological and marker-based validation.
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BibTeX
@article{blinova2026cellular,
title = {{Cellular and Tissue Mechanisms of Laser-Assisted Regeneration of Tympanic Membrane Perforation}},
author = {Ekaterina Valerievna Blinova and Igor Vyacheslavovich Lunyov and Andrey Igorevich Osipyants and Kirill Dmitrievich Blinov and Anna Vladimirovna Zolotova and Liudmila Mikhailovna Ignatova and Valeriy Michailovich Svistushkin and Valeria Vadimovna Gainutdinova and Mikhail Valerievich Svistushkin and Ekaterina Mikhailovna Kasianenko and Alexander Ivanovich Omelchenko and Aleksey Vasilevich Yuzhakov and Olga Igorevna Baum},
journal = {International journal of molecular sciences},
year = {2026},
month = oct,
doi = {10.3390/ijms27198906},
url = {https://europepmc.org/article/PMC/PMC13666236},
note = {CC BY},
}
RIS
TY - JOUR
TI - Cellular and Tissue Mechanisms of Laser-Assisted Regeneration of Tympanic Membrane Perforation
AU - Ekaterina Valerievna Blinova
AU - Igor Vyacheslavovich Lunyov
AU - Andrey Igorevich Osipyants
AU - Kirill Dmitrievich Blinov
AU - Anna Vladimirovna Zolotova
AU - Liudmila Mikhailovna Ignatova
AU - Valeriy Michailovich Svistushkin
AU - Valeria Vadimovna Gainutdinova
AU - Mikhail Valerievich Svistushkin
AU - Ekaterina Mikhailovna Kasianenko
AU - Alexander Ivanovich Omelchenko
AU - Aleksey Vasilevich Yuzhakov
AU - Olga Igorevna Baum
PY - 2026
DA - 2026/10/01
JO - International journal of molecular sciences
DO - 10.3390/ijms27198906
UR - https://europepmc.org/article/PMC/PMC13666236
AB - Chronic tympanic membrane (TM) perforations remain a significant clinical problem due to limited spontaneous healing and the drawbacks of surgical interventions. The present study aimed to evaluate the effects of low-intensity infrared laser irradiation on cellular mechanisms of regeneration and to assess its therapeutic potential in TM repair. Primary fibroblast cultures isolated from chinchilla tissue were exposed to laser radiation at wavelengths 1560 nm and 1450 nm. Cellular responses were analyzed using MTT assay, scratch test, Ki-67 immunostaining, and ELISA for collagen type II. In addition, an in vivo model of chronic TM perforation in chinchillas was used to validate the regenerative effects under physiological conditions. Laser irradiation was associated with accelerated scratch closure and increased extracellular matrix production under selected irradiation conditions, whereas metabolic and proliferative indices decreased over time, most likely reflecting culture confluence and maturation rather than cytotoxicity. In vivo, complete closure of tympanic membrane perforations was observed in all laser-treated ears in the context of collagen sponge-assisted repair, whereas scaffold-only control perforations remained unclosed. These findings support the potential of low-intensity infrared laser irradiation as a minimally invasive adjunct to scaffold-assisted tympanic membrane repair. However, the proposed involvement of endogenous progenitor cell populations should be regarded as a hypothesis requiring further histological and marker-based validation.
SN - 1422-0067
ER -