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Genes controlling polysaccharide synthesis, protein folding and basic metabolism ensure intra-host cell adaptation and replication of Yersinia enterocolitica

  • J. Brinkmann
  • J. Huang
  • P. Blümke
  • M. Aepfelbacher
  • K. Ruckdeschel1
  1. 1University Medical Center Hamburg-Eppendorf, Universitatsklinikum Hamburg-Eppendorf
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Abstract

Pathogenesis of yersiniosis comprises cell-invasive events in which the bacteria subvert the endocytic-autophagolysosomal pathway of infected host cells to create an intracellular niche for bacterial replication. To determine potential virulence factors that enable intracellular proliferation of Yersinia enterocolitica, we performed a high-throughput transposon-directed insertion site sequencing approach (TraDIS) with infected epithelial HeLa cells. In this screen, we identified seven particular genes that have either metabolic activities in disulfide bond formation (dsbA), glutamine synthesis (glnA), and energy generation (acnB), or dictate synthesis of enterobacterial common antigen ECA (webB/C/F) and LPS (wzx). Individual knockout of the identified genes impaired intracellular replication of Yersinia. Furthermore, these mutants differentially affected host cell autophagy and the acidification of Yersinia-containing vacuoles (YCVs). The results indicate that glnA, acnB, dsbA, wecB, and wecC support autophagy-promoted, intracellular replication of Yersinia. Folding of yet to be defined proteins dependent on dsbA seems to be most critically required for mediating these events. A wzx-controlled LPS synthesis pathway appears implicated in preventing YCV acidification. These results suggest that autophagy induction and adjustment of Yersinia to the intravacuolar environment result from a multifactorial adaptation process that involves formation of a protective carbohydrate envelope and basic metabolic enzymes needed for the refinement of productive intracellular replication.

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Cite

BibTeXRIS
BibTeX
@misc{brinkmann2026genes,
  title = {{Genes controlling polysaccharide synthesis, protein folding and basic metabolism ensure intra-host cell adaptation and replication of Yersinia enterocolitica}},
  author = {J. Brinkmann and J. Huang and P. Blümke and M. Aepfelbacher and K. Ruckdeschel},
  howpublished = {bioRxiv},
  year = {2026},
  month = sep,
  doi = {10.64898/2026.09.16.752068},
  url = {https://www.biorxiv.org/content/10.64898/2026.09.16.752068v1},
  note = {CC BY},
}
RIS
TY  - UNPB
TI  - Genes controlling polysaccharide synthesis, protein folding and basic metabolism ensure intra-host cell adaptation and replication of Yersinia enterocolitica
AU  - J. Brinkmann
AU  - J. Huang
AU  - P. Blümke
AU  - M. Aepfelbacher
AU  - K. Ruckdeschel
PY  - 2026
DA  - 2026/09/16
PB  - bioRxiv
DO  - 10.64898/2026.09.16.752068
UR  - https://www.biorxiv.org/content/10.64898/2026.09.16.752068v1
AB  - Pathogenesis of yersiniosis comprises cell-invasive events in which the bacteria subvert the endocytic-autophagolysosomal pathway of infected host cells to create an intracellular niche for bacterial replication. To determine potential virulence factors that enable intracellular proliferation of Yersinia enterocolitica, we performed a high-throughput transposon-directed insertion site sequencing approach (TraDIS) with infected epithelial HeLa cells. In this screen, we identified seven particular genes that have either metabolic activities in disulfide bond formation (dsbA), glutamine synthesis (glnA), and energy generation (acnB), or dictate synthesis of enterobacterial common antigen ECA (webB/C/F) and LPS (wzx). Individual knockout of the identified genes impaired intracellular replication of Yersinia. Furthermore, these mutants differentially affected host cell autophagy and the acidification of Yersinia-containing vacuoles (YCVs). The results indicate that glnA, acnB, dsbA, wecB, and wecC support autophagy-promoted, intracellular replication of Yersinia. Folding of yet to be defined proteins dependent on dsbA seems to be most critically required for mediating these events. A wzx-controlled LPS synthesis pathway appears implicated in preventing YCV acidification. These results suggest that autophagy induction and adjustment of Yersinia to the intravacuolar environment result from a multifactorial adaptation process that involves formation of a protective carbohydrate envelope and basic metabolic enzymes needed for the refinement of productive intracellular replication.
ER  -