Phage vB_KpnS_MK54: lPS-associated factors involved in phage adsorption and depolymerase-driven anti-biofilm effects
- 1College of Veterinary Medicine, Sichuan Agricultural University
- 2Key Laboratory of Agricultural Animal Diseases and Veterinary Public Health of Sichuan Province
Abstract
Understanding phage K. pneumoniae –host interactions is essential for elucidating phage infection mechanisms and developing phage-derived antibacterial strategies. This showed that lipopolysaccharide (LPS) plays an important role in phage vB_KpnS_MK54 adsorption by constructing the isogenic deletion mutants LPKPΔmsbA and LPKPΔlpxB, in which phage adsorption was reduced by 2–3 log compared with the wild-type strain. Biofilms are associated with persistent infections and increased tolerance to antimicrobial treatment, posing a significant clinical challenge. We further characterized the recombinant depolymerase Dep_Zy, which exhibited potent depolymerase and antibiofilm activities, including depolymerase activity at 0.1 μg/mL, 55% inhibition of biofilm formation at 10 μg/mL, and 30% degradation of mature biofilms at 1 μg/mL. Notably, Dep_Zy potentiated the activity of conventional antibiotics, resulting in a 1–3 log reduction in biofilm-associated bacterial counts when combined with kanamycin, gentamicin, or streptomycin. These findings elucidate the molecular basis of phage–host interactions and highlight Dep_Zy as a promising enzymatic strategy for controlling multidrug-resistant Klebsiella pneumoniae through biofilm disruption and antibiotic potentiation.
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BibTeX
@article{zeng2026phage,
title = {{Phage vB_KpnS_MK54: lPS-associated factors involved in phage adsorption and depolymerase-driven anti-biofilm effects}},
author = {Yan Zeng and Zhaomin Wu and Zhuangkan Yan and Yin Wang and Zexiao Yang and Yixin Huang and Suizhong Cao and Xueping Yao},
journal = {Frontiers in Microbiology},
year = {2026},
month = oct,
doi = {10.3389/fmicb.2026.1963357},
url = {https://www.frontiersin.org/articles/10.3389/fmicb.2026.1963357/full},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Phage vB_KpnS_MK54: lPS-associated factors involved in phage adsorption and depolymerase-driven anti-biofilm effects
AU - Yan Zeng
AU - Zhaomin Wu
AU - Zhuangkan Yan
AU - Yin Wang
AU - Zexiao Yang
AU - Yixin Huang
AU - Suizhong Cao
AU - Xueping Yao
PY - 2026
DA - 2026/10/09
JO - Frontiers in Microbiology
DO - 10.3389/fmicb.2026.1963357
UR - https://www.frontiersin.org/articles/10.3389/fmicb.2026.1963357/full
AB - Understanding phage K. pneumoniae –host interactions is essential for elucidating phage infection mechanisms and developing phage-derived antibacterial strategies. This showed that lipopolysaccharide (LPS) plays an important role in phage vB_KpnS_MK54 adsorption by constructing the isogenic deletion mutants LPKPΔmsbA and LPKPΔlpxB, in which phage adsorption was reduced by 2–3 log compared with the wild-type strain. Biofilms are associated with persistent infections and increased tolerance to antimicrobial treatment, posing a significant clinical challenge. We further characterized the recombinant depolymerase Dep_Zy, which exhibited potent depolymerase and antibiofilm activities, including depolymerase activity at 0.1 μg/mL, 55% inhibition of biofilm formation at 10 μg/mL, and 30% degradation of mature biofilms at 1 μg/mL. Notably, Dep_Zy potentiated the activity of conventional antibiotics, resulting in a 1–3 log reduction in biofilm-associated bacterial counts when combined with kanamycin, gentamicin, or streptomycin. These findings elucidate the molecular basis of phage–host interactions and highlight Dep_Zy as a promising enzymatic strategy for controlling multidrug-resistant Klebsiella pneumoniae through biofilm disruption and antibiotic potentiation.
SN - 1664-302X
ER -