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Peer-reviewedCC BY-NC 4.0Yeast (Chichester, England)

Transposons as Tools for Future Genome Engineering in Yeasts and Filamentous Fungi

  • Bingyin Peng1,2
  • Masahiro Tominaga3,4
  • Chengqiang Wang5
  • Robert E. Speight6,7
  • Jun Ishii3,4,8
8 affiliations
  1. 1School of Biology and Environmental Science, Faculty of Science Queensland University of Technology Brisbane Queensland Australia
  2. 2Australian Institute for Bioengineering and Nanotechnology (AIBN) The University of Queensland Brisbane Queensland Australia
  3. 3Engineering Biology Research Centre Kobe University Nada Kobe Japan
  4. 4Graduate School of Science, Technology and Innovation Kobe University Nada Kobe Japan
  5. 5College of Life Sciences, National Engineering Research Center for Efficient Utilisation of Soil and Fertiliser Resources Shandong Agricultural University Tai'an Shandong China
  6. 6CSIRO Advanced Engineering Biology Future Science Platform Dutton Park Queensland Australia
  7. 7ARC Centre of Excellence in Synthetic Biology Brisbane Queensland Australia
  8. 8Department of Chemical Science and Engineering, Faculty of Engineering Kobe University Nada Kobe Japan
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Abstract

Transposons are fundamental genetic elements that have profoundly shaped the architecture of eukaryotic genomes. Yeasts and filamentous fungi have emerged as important chassis organisms for bioingredient production in synthetic biology and metabolic engineering. In this review, we summarise the current understanding and future opportunities in the development of transposon‐based tools for genome engineering in these fungal systems. Fungal inverted terminal repeat (ITR) DNA transposons, as well as long terminal repeat (LTR) and non‐LTR retrotransposons, can accelerate genomic mutagenesis, facilitating the screening of superior genotypes and phenotypes. CRISPR‐associated transposons (CASTs) hold considerable potential for site‐specific integration of large transgenes, bypassing the limitations imposed by low homologous recombination (HR) efficiency in non‐Saccharomyces hosts. Overall, transposon‐based tools represent a valuable and underexplored avenue to accelerate genome engineering and strain development in yeasts and filamentous fungi.

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Cite

BibTeXRIS
BibTeX
@article{peng2026transposons,
  title = {{Transposons as Tools for Future Genome Engineering in Yeasts and Filamentous Fungi}},
  author = {Bingyin Peng and Masahiro Tominaga and Chengqiang Wang and Robert E. Speight and Jun Ishii},
  journal = {Yeast (Chichester, England)},
  year = {2026},
  month = jul,
  doi = {10.1002/yea.70036},
  url = {https://doi.org/10.1002/yea.70036},
  note = {CC BY-NC 4.0},
}
RIS
TY  - JOUR
TI  - Transposons as Tools for Future Genome Engineering in Yeasts and Filamentous Fungi
AU  - Bingyin Peng
AU  - Masahiro Tominaga
AU  - Chengqiang Wang
AU  - Robert E. Speight
AU  - Jun Ishii
PY  - 2026
DA  - 2026/07/13
JO  - Yeast (Chichester, England)
DO  - 10.1002/yea.70036
UR  - https://doi.org/10.1002/yea.70036
AB  - Transposons are fundamental genetic elements that have profoundly shaped the architecture of eukaryotic genomes. Yeasts and filamentous fungi have emerged as important chassis organisms for bioingredient production in synthetic biology and metabolic engineering. In this review, we summarise the current understanding and future opportunities in the development of transposon‐based tools for genome engineering in these fungal systems. Fungal inverted terminal repeat (ITR) DNA transposons, as well as long terminal repeat (LTR) and non‐LTR retrotransposons, can accelerate genomic mutagenesis, facilitating the screening of superior genotypes and phenotypes. CRISPR‐associated transposons (CASTs) hold considerable potential for site‐specific integration of large transgenes, bypassing the limitations imposed by low homologous recombination (HR) efficiency in non‐Saccharomyces hosts. Overall, transposon‐based tools represent a valuable and underexplored avenue to accelerate genome engineering and strain development in yeasts and filamentous fungi.
SN  - 0749-503X
SN  - 1097-0061
ER  -