Green synthesis of silver nanoparticles from Buddleja asiatica L. and their multifunctional applications for industrial and bio-based products
14 affiliations
- 1Department of Botany, Women University of Azad Jammu and Kashmir, Bagh, Azad Kashmir, 12500, Pakistan
- 2Birmingham Institute of Forest Research, University of Birmingham, Birmingham, B15 2TT, UK
- 3Department of Botany, University of Kotli, Azad Jammu and Kashmir, Kotli, 11100, Pakistan
- 4Department of Botany, University of Poonch, Rawalakot, 12350, Pakistan
- 5Department of Plant Sciences, Quaid-e-Azam University, Islamabad, 45320, Pakistan
- 6Department of Botany, University of Chakwal, Chakwal, 48800, Pakistan
- 7Department of Botany, The Islamia University of Bahawalpur, Bahawalpur, 63100, Pakistan. [email protected]
- 8Agricultural Biotechnology Department, College of Agriculture and Food Sciences, King Faisal University, 31982, Al-Ahsa, Saudi Arabia. [email protected]
- 9Department of Biology, College of Science, Qassim University, Qassim, Saudi Arabia
- 10Biological Sciences Department, Faculty of Science and Arts, King Abdulaziz University, Rabigh, 21911, Saudi Arabia
- 11Institute of Environmental Engineering, RUDN University, Miklukho-Maklaya St, Moscow, Russia, 117198
- 12Division of Research and Development, Lovely Professional University, Phagwara, Punjab, 144001, India. [email protected]
- 13Kardan University, Parwane Du, 1001, Kabul, Afghanistan. [email protected]
- 14University Centre for Research & Development, Chandigarh University, Gharuan, Mohali, Punjab, 140413, India. [email protected]
Abstract
Nanotechnology provides innovative solutions to biomedical and agricultural challenges, and silver nanoparticles (AgNPs) are of particular interest due to their broad-spectrum bioactivity. This study aimed to green-synthesize AgNPs using Buddleja asiatica leaf extract, characterize the nanoparticles, and evaluate antibacterial, antioxidant, anticancer, and phytotoxic/phytostimulatory activities. AgNP formation was confirmed by UV–visible spectroscopy (SPR peak at 420 nm). XRD verified a crystalline face-centered cubic structure, while SEM showed predominantly near-spherical nanoparticles (10–22 nm). FTIR indicated plant-derived functional groups involved in stabilization. Biologically, BA-AgNPs exhibited strong antibacterial activity against a Gram-positive clinical isolate ( Streptococcus sp.) and Gram-negative bacteria, with the largest inhibition zone of 14 ± 0.57 mm at 0.9 mg/mL. Antioxidant activity reached 88.12% DPPH scavenging at 100 ppm, compared with 80.90% for ascorbic acid under the same assay conditions. BA-AgNPs showed concentration-dependent cytotoxicity in RD cells, with cell death ranging from 87.00% at 100 mg/mL to 5.33% at 0.39062 mg/mL; however, IC 50 is reported only as an estimated range of 6.25–12.5 mg/mL based on the concentration interval where cytotoxicity crossed 50%. In wheat, lower concentrations showed phytostimulatory trends, with the strongest response at 50 mg/L, while 1000 mg/L caused inhibitory effects. This is the first comprehensive report on B. asiatica –mediated AgNP synthesis and multifunctional bioactivity, highlighting BA-AgNPs as a sustainable nanomaterial for antimicrobial, antioxidant, anticancer, and agricultural applications, while emphasizing the need for future stability, mechanistic, and translational safety studies.
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BibTeX
@article{fatima2026green,
title = {{Green synthesis of silver nanoparticles from Buddleja asiatica L. and their multifunctional applications for industrial and bio-based products}},
author = {Maryum Fatima and Muhammad Shoaib Amjad and Ansar Mehmood and Samiullah Khan and Zakia Binish and Huma Arshad and Huma Qureshi and Tauseef Anwar and Hossam Saad EL-BELTAGI and Ibtisam Mohammed Alsudays and Khalid Hasan Alamer and Nazih Y. Rebouh and Mohd Asif Shah},
journal = {Scientific Reports},
year = {2026},
month = jul,
doi = {10.1038/s41598-026-57982-5},
url = {https://doi.org/10.1038/s41598-026-57982-5},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Green synthesis of silver nanoparticles from Buddleja asiatica L. and their multifunctional applications for industrial and bio-based products
AU - Maryum Fatima
AU - Muhammad Shoaib Amjad
AU - Ansar Mehmood
AU - Samiullah Khan
AU - Zakia Binish
AU - Huma Arshad
AU - Huma Qureshi
AU - Tauseef Anwar
AU - Hossam Saad EL-BELTAGI
AU - Ibtisam Mohammed Alsudays
AU - Khalid Hasan Alamer
AU - Nazih Y. Rebouh
AU - Mohd Asif Shah
PY - 2026
DA - 2026/07/30
JO - Scientific Reports
DO - 10.1038/s41598-026-57982-5
UR - https://doi.org/10.1038/s41598-026-57982-5
AB - Nanotechnology provides innovative solutions to biomedical and agricultural challenges, and silver nanoparticles (AgNPs) are of particular interest due to their broad-spectrum bioactivity. This study aimed to green-synthesize AgNPs using Buddleja asiatica leaf extract, characterize the nanoparticles, and evaluate antibacterial, antioxidant, anticancer, and phytotoxic/phytostimulatory activities. AgNP formation was confirmed by UV–visible spectroscopy (SPR peak at 420 nm). XRD verified a crystalline face-centered cubic structure, while SEM showed predominantly near-spherical nanoparticles (10–22 nm). FTIR indicated plant-derived functional groups involved in stabilization. Biologically, BA-AgNPs exhibited strong antibacterial activity against a Gram-positive clinical isolate ( Streptococcus sp.) and Gram-negative bacteria, with the largest inhibition zone of 14 ± 0.57 mm at 0.9 mg/mL. Antioxidant activity reached 88.12% DPPH scavenging at 100 ppm, compared with 80.90% for ascorbic acid under the same assay conditions. BA-AgNPs showed concentration-dependent cytotoxicity in RD cells, with cell death ranging from 87.00% at 100 mg/mL to 5.33% at 0.39062 mg/mL; however, IC 50 is reported only as an estimated range of 6.25–12.5 mg/mL based on the concentration interval where cytotoxicity crossed 50%. In wheat, lower concentrations showed phytostimulatory trends, with the strongest response at 50 mg/L, while 1000 mg/L caused inhibitory effects. This is the first comprehensive report on B. asiatica –mediated AgNP synthesis and multifunctional bioactivity, highlighting BA-AgNPs as a sustainable nanomaterial for antimicrobial, antioxidant, anticancer, and agricultural applications, while emphasizing the need for future stability, mechanistic, and translational safety studies.
SN - 2045-2322
ER -