Skip to content
DraconiticResearch
Search Sources API
190,335 studies31 sourcesUpdated 23 min ago
Peer-reviewedCC BYAdvanced science (Weinheim, Baden-Wurttemberg, Germany)

The Characteristics of Tumor Microenvironment by the Single Cell Profile in Endometrial Cancer of no Specific Molecular Profile (NSMP) Subtype.

  • Xinhui Yuan1,2
  • Zhengyao Zhai1,2
  • Chuyu Jing1,2
  • Wei Zhang1,2
  • Wenjuan Tian1,2
  • Yulan Ren1,2
  1. 1Department of Gynecologic Oncology, Fudan University Shanghai Cancer Center, Shanghai, China.
  2. 2Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.
Read full textPDFDOICite

Abstract

Endometrial cancer (EC) of no specific molecular profile (NSMP) subtype lacks specific molecular features, and the relationship of tumor microenvironment (TME) and relapse in NSMP EC remains incompletely understood. This study integrates single-nucleus RNA sequencing, spatial transcriptomics, and multiplex immunohistochemistry to elucidate the TME and mechanisms of relapse in NSMP EC. The results showed reduced immune infiltration and increased immune-suppressive cells, including SPP1+ macrophages and regulatory T cells (Tregs) in NSMP EC. More epithelial cell clusters with high stemness scores are observed in primary NSMP EC tumors collected before recurrence. In NSMP EC, tip endothelial cells and MMP11+ LEF1+ cancer-associated fibroblasts (CAFs) are linked with angiogenesis and epithelial-mesenchymal transition, and CD4+ T cells remain in a stress-response state and are functionally impaired. Interestingly, a subset of tumor-associated macrophages (TAMs) characterized by SPP1 expression is discovered, which may remodel the tumor microenvironment by potentially promoting CD4+ T cells stress and maintaining epithelial cells stemness through SPP1-CD44, thereby potentially contributing to the proliferation and migration of tumor cells, which has verified by in vitro experiments. This study advances the mechanistic understanding of recurrence-related TME and provides a foundation for the development of personalized treatments for NSMP EC patients.

Related

  1. Reclassifying No Specific Molecular Profile Endometrial Cancer: A Narrative Review of Molecular Heterogeneity, Prognostic Refinement, and Clinical Management
    Yujing You, Yan Zhang
    CancersPeer-reviewedCC BYFull textObstetrics, gynaecology & paediatricsEurope PMCCrossref
  2. Clinicopathologic Characteristics, Molecular Profile, Treatment, and Recurrence in Endometrial Cancer with Pelvic Lymph Node Isolated Tumor Cells
    Haley D. Frerichs, Cheng-Han Lee, Ericka Wiebe +1
    CancersPeer-reviewedCC BY 4.0Full textObstetrics, gynaecology & paediatricsCrossrefEurope PMC
  3. Clinical and pathological characteristics, immune microenvironment, and survival analysis of molecular subtypes of surgically resected small cell lung cancer.
    Juncai Tian, Jiaxin He, Huohuan Tian +2
    BMC cancerPeer-reviewedCC BY-NC-NDFull textOncologyEurope PMCDOAJ
  4. Abnormal uterine bleeding (AUB) is not a single clinical entity: different clinical causes are associated with distinct cellular and molecular endometrial characteristics
    V. Jain, F. Petitprez, J. Chung +5
    medRxivPreprintCC BYFull textObstetrics, gynaecology & paediatricsmedRxiv
  5. Hypothesis-Generating Single-Cell Analysis of the Endometrial Cancer Immune Microenvironment With Complementary Multiomics Evidence.
    Xiaolan Ouyang, Xiaoli Guo, Xiaoxi Yang +1
    International journal of genomicsPeer-reviewedCC BYFull textOncologyEurope PMC

Cite

BibTeXRIS
BibTeX
@article{yuan2026characteristics,
  title = {{The Characteristics of Tumor Microenvironment by the Single Cell Profile in Endometrial Cancer of no Specific Molecular Profile (NSMP) Subtype.}},
  author = {Xinhui Yuan and Zhengyao Zhai and Chuyu Jing and Wei Zhang and Wenjuan Tian and Yulan Ren},
  journal = {Advanced science (Weinheim, Baden-Wurttemberg, Germany)},
  year = {2026},
  month = oct,
  doi = {10.1002/advs.78332},
  url = {https://europepmc.org/article/MED/42902496},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - The Characteristics of Tumor Microenvironment by the Single Cell Profile in Endometrial Cancer of no Specific Molecular Profile (NSMP) Subtype.
AU  - Xinhui Yuan
AU  - Zhengyao Zhai
AU  - Chuyu Jing
AU  - Wei Zhang
AU  - Wenjuan Tian
AU  - Yulan Ren
PY  - 2026
DA  - 2026/10/20
JO  - Advanced science (Weinheim, Baden-Wurttemberg, Germany)
DO  - 10.1002/advs.78332
UR  - https://europepmc.org/article/MED/42902496
AB  - Endometrial cancer (EC) of no specific molecular profile (NSMP) subtype lacks specific molecular features, and the relationship of tumor microenvironment (TME) and relapse in NSMP EC remains incompletely understood. This study integrates single-nucleus RNA sequencing, spatial transcriptomics, and multiplex immunohistochemistry to elucidate the TME and mechanisms of relapse in NSMP EC. The results showed reduced immune infiltration and increased immune-suppressive cells, including SPP1+ macrophages and regulatory T cells (Tregs) in NSMP EC. More epithelial cell clusters with high stemness scores are observed in primary NSMP EC tumors collected before recurrence. In NSMP EC, tip endothelial cells and MMP11+ LEF1+ cancer-associated fibroblasts (CAFs) are linked with angiogenesis and epithelial-mesenchymal transition, and CD4+ T cells remain in a stress-response state and are functionally impaired. Interestingly, a subset of tumor-associated macrophages (TAMs) characterized by SPP1 expression is discovered, which may remodel the tumor microenvironment by potentially promoting CD4+ T cells stress and maintaining epithelial cells stemness through SPP1-CD44, thereby potentially contributing to the proliferation and migration of tumor cells, which has verified by in vitro experiments. This study advances the mechanistic understanding of recurrence-related TME and provides a foundation for the development of personalized treatments for NSMP EC patients.
SN  - 2198-3844
ER  -