Microbial Community Succession and Its Environmental Response in Bighead Carp (Hypophthalmichthys nobilis) Muscle During Low-Temperature Modified Atmosphere Transportation.
- 1Chongqing Fishery Sciences Research Institute, Chongqing 400020, China.
- 2Animal Microecology Institute, College of Veterinary, Sichuan Agricultural University, Chengdu 611130, China.
Abstract
Microbial community succession contributes to quality changes in fresh freshwater fish during cold-chain transportation. In this study, bighead carp (Hypophthalmichthys nobilis) was used as the research material. Using 16S rRNA high-throughput sequencing, we characterized microbial community dynamics in fish muscle after 12 h and 24 h of simulated transportation under combined low-temperature (0 °C, 4 °C, 8 °C) and gradient CO₂ modified atmosphere packaging (MAP) treatments. We further identified key environmental factors associated with shifts in bacterial communities during cold-chain logistics. The results showed that transportation temperature was the primary factor structuring microbial communities in bighead carp muscle, with stronger effects than MAP composition and transportation duration. Under 8 °C temperature conditions, low-to-moderate CO₂ MAP corresponded with elevated microbial diversity and spoilage-associated succession, alongside enrichment of spoilage taxa including Pseudomonas. High-CO₂ (80%) MAP altered community composition and reduced community complexity under low-temperature conditions, and the community changes exhibited a time-dependent pattern during extended transport. The combined treatment of 0 °C and 80% CO₂ MAP generated distinct microbial succession profiles within 24 h and lowered the relative abundance of Pseudomonas. This study described the response patterns of bighead carp muscle microbiota to coupled low-temperature and MAP treatments and characterized community succession during cold-chain transport. The findings provide microbiota-based references for understanding microbial variation in freshwater fish cold-chain logistics.
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BibTeX
@article{dan2026microbial,
title = {{Microbial Community Succession and Its Environmental Response in Bighead Carp (Hypophthalmichthys nobilis) Muscle During Low-Temperature Modified Atmosphere Transportation.}},
author = {Yan Dan and Zhen Liu and Shuang Li and Chongjiang Hu and Yuankun Chen and Yan Zeng and Yibo Zhang},
journal = {Foods (Basel, Switzerland)},
year = {2026},
month = sep,
doi = {10.3390/foods15193430},
url = {https://europepmc.org/article/MED/42883539},
note = {CC BY},
}
RIS
TY - JOUR
TI - Microbial Community Succession and Its Environmental Response in Bighead Carp (Hypophthalmichthys nobilis) Muscle During Low-Temperature Modified Atmosphere Transportation.
AU - Yan Dan
AU - Zhen Liu
AU - Shuang Li
AU - Chongjiang Hu
AU - Yuankun Chen
AU - Yan Zeng
AU - Yibo Zhang
PY - 2026
DA - 2026/09/25
JO - Foods (Basel, Switzerland)
DO - 10.3390/foods15193430
UR - https://europepmc.org/article/MED/42883539
AB - Microbial community succession contributes to quality changes in fresh freshwater fish during cold-chain transportation. In this study, bighead carp (Hypophthalmichthys nobilis) was used as the research material. Using 16S rRNA high-throughput sequencing, we characterized microbial community dynamics in fish muscle after 12 h and 24 h of simulated transportation under combined low-temperature (0 °C, 4 °C, 8 °C) and gradient CO₂ modified atmosphere packaging (MAP) treatments. We further identified key environmental factors associated with shifts in bacterial communities during cold-chain logistics. The results showed that transportation temperature was the primary factor structuring microbial communities in bighead carp muscle, with stronger effects than MAP composition and transportation duration. Under 8 °C temperature conditions, low-to-moderate CO₂ MAP corresponded with elevated microbial diversity and spoilage-associated succession, alongside enrichment of spoilage taxa including Pseudomonas. High-CO₂ (80%) MAP altered community composition and reduced community complexity under low-temperature conditions, and the community changes exhibited a time-dependent pattern during extended transport. The combined treatment of 0 °C and 80% CO₂ MAP generated distinct microbial succession profiles within 24 h and lowered the relative abundance of Pseudomonas. This study described the response patterns of bighead carp muscle microbiota to coupled low-temperature and MAP treatments and characterized community succession during cold-chain transport. The findings provide microbiota-based references for understanding microbial variation in freshwater fish cold-chain logistics.
SN - 2304-8158
ER -