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Peer-reviewedCC BY-NC-NDBMC cancer

EIF3B is associated with m⁶A-related regulation of E2F7 and promotes stemness in glioblastoma multiforme.

  • Jiao Meng1,2
  • Xinyi Jiang1
  • Yaling Hu1,2
  • Jinyue Tian1
  • Zhenkun Yang3,4
  • Zhening Pu5,6
  • Ying Yin7,8
8 affiliations
  1. 1Department of Laboratory Medicine, Wuxi People's Hospital, Wuxi Medical Center, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Nanjing Medical University, Wuxi, Jiangsu, 214023, China.
  2. 2Center of Clinical Research, Wuxi People's Hospital, Wuxi Medical Center, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Nanjing Medical University, Wuxi, Jiangsu, 214023, China.
  3. 3Department of Laboratory Medicine, Wuxi People's Hospital, Wuxi Medical Center, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Nanjing Medical University, Wuxi, Jiangsu, 214023, China. [email protected].
  4. 4Center of Clinical Research, Wuxi People's Hospital, Wuxi Medical Center, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Nanjing Medical University, Wuxi, Jiangsu, 214023, China. [email protected].
  5. 5Department of Laboratory Medicine, Wuxi People's Hospital, Wuxi Medical Center, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Nanjing Medical University, Wuxi, Jiangsu, 214023, China. [email protected].
  6. 6Center of Clinical Research, Wuxi People's Hospital, Wuxi Medical Center, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Nanjing Medical University, Wuxi, Jiangsu, 214023, China. [email protected].
  7. 7Department of Laboratory Medicine, Wuxi People's Hospital, Wuxi Medical Center, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Nanjing Medical University, Wuxi, Jiangsu, 214023, China. [email protected].
  8. 8Center of Clinical Research, Wuxi People's Hospital, Wuxi Medical Center, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Nanjing Medical University, Wuxi, Jiangsu, 214023, China. [email protected].
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Abstract

Background

E2F transcription factors are key regulators of the cell cycle and play an important role in tumorigenesis. N6-methyladenosine (m⁶A), the most prevalent internal RNA modification in eukaryotic cells, is also crucial for cancer progression. However, the interplay between E2F family members and m⁶A modification in glioblastoma multiforme (GBM) remains poorly understood.Methods

We applied weighted gene co-expression network analysis (WGCNA) to identify m⁶A-related gene modules co-expressed with E2F7, a gene that is significantly associated with adverse prognosis and tumor stemness. Through intersection analysis, eukaryotic translation initiation factor 3 subunit B (EIF3B) emerged as a hub gene, showing a strong positive correlation with E2F7 in GBM datasets. Functional validation was performed in GBM cells to assess the regulatory relationship between EIF3B and E2F7, including examination of mRNA and protein expression, mRNA stability, and m⁶A modification levels. GBM stemness was evaluated by tumor sphere formation assays and detection of stemness-associated markers.Results

Functional validation revealed that EIF3B knockdown reduced E2F7 mRNA and protein expression, promoted E2F7 mRNA degradation, and decreased its m⁶A modification, suggesting an association between EIF3B and m⁶A-related regulation of E2F7 stability. Furthermore, EIF3B knockdown markedly suppressed GBM stemness, as demonstrated by impaired tumor sphere formation and downregulation of stemness-associated markers. Clinically, EIF3B expression increased with glioma grade and correlated with poor prognosis.Conclusions

Collectively, these findings suggest that the EIF3B-E2F7 axis is associated with m⁶A-related regulation in GBM and may warrant further investigation as a candidate for therapeutic targeting.

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Cite

BibTeXRIS
BibTeX
@article{meng2026eif3b,
  title = {{EIF3B is associated with m⁶A-related regulation of E2F7 and promotes stemness in glioblastoma multiforme.}},
  author = {Jiao Meng and Xinyi Jiang and Yaling Hu and Jinyue Tian and Zhenkun Yang and Zhening Pu and Ying Yin},
  journal = {BMC cancer},
  year = {2026},
  month = jul,
  doi = {10.1186/s12885-026-16552-8},
  url = {https://europepmc.org/article/MED/42919175},
  note = {CC BY-NC-ND},
}
RIS
TY  - JOUR
TI  - EIF3B is associated with m⁶A-related regulation of E2F7 and promotes stemness in glioblastoma multiforme.
AU  - Jiao Meng
AU  - Xinyi Jiang
AU  - Yaling Hu
AU  - Jinyue Tian
AU  - Zhenkun Yang
AU  - Zhening Pu
AU  - Ying Yin
PY  - 2026
DA  - 2026/07/01
JO  - BMC cancer
DO  - 10.1186/s12885-026-16552-8
UR  - https://europepmc.org/article/MED/42919175
AB  - Background  E2F transcription factors are key regulators of the cell cycle and play an important role in tumorigenesis. N6-methyladenosine (m⁶A), the most prevalent internal RNA modification in eukaryotic cells, is also crucial for cancer progression. However, the interplay between E2F family members and m⁶A modification in glioblastoma multiforme (GBM) remains poorly understood.Methods  We applied weighted gene co-expression network analysis (WGCNA) to identify m⁶A-related gene modules co-expressed with E2F7, a gene that is significantly associated with adverse prognosis and tumor stemness. Through intersection analysis, eukaryotic translation initiation factor 3 subunit B (EIF3B) emerged as a hub gene, showing a strong positive correlation with E2F7 in GBM datasets. Functional validation was performed in GBM cells to assess the regulatory relationship between EIF3B and E2F7, including examination of mRNA and protein expression, mRNA stability, and m⁶A modification levels. GBM stemness was evaluated by tumor sphere formation assays and detection of stemness-associated markers.Results  Functional validation revealed that EIF3B knockdown reduced E2F7 mRNA and protein expression, promoted E2F7 mRNA degradation, and decreased its m⁶A modification, suggesting an association between EIF3B and m⁶A-related regulation of E2F7 stability. Furthermore, EIF3B knockdown markedly suppressed GBM stemness, as demonstrated by impaired tumor sphere formation and downregulation of stemness-associated markers. Clinically, EIF3B expression increased with glioma grade and correlated with poor prognosis.Conclusions  Collectively, these findings suggest that the EIF3B-E2F7 axis is associated with m⁶A-related regulation in GBM and may warrant further investigation as a candidate for therapeutic targeting.
SN  - 1471-2407
ER  -