Cerium Dioxide Nanoparticles Modulate Ribosomal DNA Transcriptional Activity in Cultured Human Skin Fibroblasts
- 1Petrovsky Russian Scientific Center of Surgery, Institute of Biology of Aging and Healthy Longevity Medicine with Clinic of Predictive Medicine, Moscow 119435, Russia
- 2Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Science, Moscow 119435, Russia
- 3Center of Digital Health and AI in Medicine, I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University), Moscow 119991, Russia
Abstract
The study investigates the effect of cerium dioxide (CeO2) nanoparticles on ribosomal DNA (rDNA) transcriptional activity and the status of ribosomal genes in human skin fibroblasts. Nano-CeO2 has attracted attention due to its antioxidant and redox properties; however, its impact on rDNA has so far remained virtually unexplored. Fibroblasts from ten adult donors were incubated with nano-CeO2 for 3 and 24 h, and the copy number of ribosomal genes and their functional state were assessed using real-time quantitative PCR and non-radioactive quantitative hybridization; the interaction of the nanoparticles with the cells was also assessed by fluorescence microscopy. It was shown that exposure to CeO2 nanoparticles leads to functional changes in rDNA transcription and rRNA abundance, indicating modulation of ribosomal biogenesis and consistent with a model of altered balance between transcriptionally active and inactive rDNA copies, although this study did not directly measure ribosome content or rDNA fraction distribution. The observed effects are likely mediated by the antioxidant and redox properties of the nanoparticles. Thus, nano-CeO2 can be considered a compound capable of modulating the functional state of ribosomal genes in normal human cells.
Related
- Hemp Seed-Derived Vesicle-like Nanoparticles Modulate Dermal Collagen Homeostasis and Attenuate UVB-Induced Photoaging-Related Responses
- Green Synthesis of Silver Nanoparticles Using Ammoides pusilla Extract: Optimization, Characterization, and Antimicrobial Activity
- Comparative antifungal activity of plant- and microbe-mediated silver nanoparticles against Fusarium oxysporum f. sp. cubense
- Biogenic synthesis of silver nanoparticles using Prosopis farcta: a green nanotechnology approach for enhanced antimicrobial activity
- Chiral by nature: chiroptical activity in Fe3O4 magnetic nanoparticles from bacterial biomineralization
BibTeX
@article{veiko2026cerium,
title = {{Cerium Dioxide Nanoparticles Modulate Ribosomal DNA Transcriptional Activity in Cultured Human Skin Fibroblasts}},
author = {Natalia N. Veiko and Elizaveta S. Ershova and Madina M. Sozarukova and Elena V. Proskurnina and Larisa V. Kameneva and Edmund V. Kostyuk and Tatiana M. Rozhnova and Yuriy L. Orlov and Svetlana Yu. Vikhrova and Alesya M. Drozhdinina and Vladimir K. Ivanov and Svetlana V. Kostyuk},
journal = {International journal of molecular sciences},
year = {2026},
month = oct,
doi = {10.3390/ijms27198913},
url = {https://europepmc.org/article/PMC/PMC13665379},
note = {CC BY},
}
RIS
TY - JOUR
TI - Cerium Dioxide Nanoparticles Modulate Ribosomal DNA Transcriptional Activity in Cultured Human Skin Fibroblasts
AU - Natalia N. Veiko
AU - Elizaveta S. Ershova
AU - Madina M. Sozarukova
AU - Elena V. Proskurnina
AU - Larisa V. Kameneva
AU - Edmund V. Kostyuk
AU - Tatiana M. Rozhnova
AU - Yuriy L. Orlov
AU - Svetlana Yu. Vikhrova
AU - Alesya M. Drozhdinina
AU - Vladimir K. Ivanov
AU - Svetlana V. Kostyuk
PY - 2026
DA - 2026/10/01
JO - International journal of molecular sciences
DO - 10.3390/ijms27198913
UR - https://europepmc.org/article/PMC/PMC13665379
AB - The study investigates the effect of cerium dioxide (CeO2) nanoparticles on ribosomal DNA (rDNA) transcriptional activity and the status of ribosomal genes in human skin fibroblasts. Nano-CeO2 has attracted attention due to its antioxidant and redox properties; however, its impact on rDNA has so far remained virtually unexplored. Fibroblasts from ten adult donors were incubated with nano-CeO2 for 3 and 24 h, and the copy number of ribosomal genes and their functional state were assessed using real-time quantitative PCR and non-radioactive quantitative hybridization; the interaction of the nanoparticles with the cells was also assessed by fluorescence microscopy. It was shown that exposure to CeO2 nanoparticles leads to functional changes in rDNA transcription and rRNA abundance, indicating modulation of ribosomal biogenesis and consistent with a model of altered balance between transcriptionally active and inactive rDNA copies, although this study did not directly measure ribosome content or rDNA fraction distribution. The observed effects are likely mediated by the antioxidant and redox properties of the nanoparticles. Thus, nano-CeO2 can be considered a compound capable of modulating the functional state of ribosomal genes in normal human cells.
SN - 1422-0067
ER -