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Peer-reviewedCC BY-NC 4.0STAR Protocols

Protocol for constructing barcoded Saccharomyces cerevisiae strains and performing pooled mating experiments

  • Sivan Kaminski Strauss1
  • Yitzhak Pilpel1
  • Orna Dahan1
  1. 1Department of Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
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Abstract

Barcoded Saccharomyces cerevisiae strains enable pooled genetic analyses across many genotypes. Here, we describe a protocol for generating stable barcoded haploid strains from natural diploid isolates. The workflow includes high-throughput transformation, selection and validation of transformants, followed by sporulation and recovery of stable haploids. We also describe phenotypic and molecular validation methods. An optional en masse mating procedure generates pooled diploid offspring.

For complete details on the use and execution of this protocol, please refer to Strauss et al.1

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BibTeXRIS
BibTeX
@article{strauss2026protocol,
  title = {{Protocol for constructing barcoded Saccharomyces cerevisiae strains and performing pooled mating experiments}},
  author = {Sivan Kaminski Strauss and Yitzhak Pilpel and Orna Dahan},
  journal = {STAR Protocols},
  year = {2026},
  month = oct,
  doi = {10.1016/j.xpro.2026.104905},
  url = {https://doi.org/10.1016/j.xpro.2026.104905},
  note = {CC BY-NC 4.0},
}
RIS
TY  - JOUR
TI  - Protocol for constructing barcoded Saccharomyces cerevisiae strains and performing pooled mating experiments
AU  - Sivan Kaminski Strauss
AU  - Yitzhak Pilpel
AU  - Orna Dahan
PY  - 2026
DA  - 2026/10/21
JO  - STAR Protocols
DO  - 10.1016/j.xpro.2026.104905
UR  - https://doi.org/10.1016/j.xpro.2026.104905
AB  - Barcoded Saccharomyces cerevisiae strains enable pooled genetic analyses across many genotypes. Here, we describe a protocol for generating stable barcoded haploid strains from natural diploid isolates. The workflow includes high-throughput transformation, selection and validation of transformants, followed by sporulation and recovery of stable haploids. We also describe phenotypic and molecular validation methods. An optional en masse mating procedure generates pooled diploid offspring.  For complete details on the use and execution of this protocol, please refer to Strauss et al.1
SN  - 2666-1667
ER  -