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Peer-reviewedCC BYCell Reports Physical Science

COWFISH2A+ as a versatile and accessible platform for digital bioanalysis

  • Tatsuya Iida1
  • Hajime Shinoda2
  • Mami Yoshimura2
  • Asami Makino2
  • Taichi Yoshimura3
  • Masaharu Nakazawa3
  • Hiroshi Hayakawa3
  • Yasushi Kogo2
  • Kosuke Miyauchi4
  • Takeshi Noda5
  • Toshiaki Ban6
  • Hiroaki Takeuchi7
2 more authors
  • Masashi Toyoda8
  • Rikiya Watanabe1
8 affiliations
  1. 1Pioneering Research Institute (PRI), RIKEN, Saitama, Japan; Corresponding author
  2. 2Pioneering Research Institute (PRI), RIKEN, Saitama, Japan
  3. 3BIOTEC Co., Ltd, Tokyo, Japan
  4. 4RIKEN Center for Integrative Medical Sciences (IMS), RIKEN, Kanagawa, Japan
  5. 5Institute for life and medical sciences, Kyoto University, Kyoto, Japan
  6. 6Isumi Medical Center, Chiba, Japan
  7. 7Center for Infectious Disease and Analysis (TCIDEA), Institute of Science Tokyo, Tokyo, Japan
  8. 8Tokyo Metropolitan Institute for Geriatrics and Gerontology, Tokyo, Japan
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Abstract

Digital bioanalysis enables single-molecule detection of biomolecules and holds transformative potential across the life sciences and clinical diagnostics. However, its broader translation remains limited by accessibility and versatility. Here, we present COWFISH2A+, a compact, fully automated device integrating wide-field imaging with a custom dispensing module. Using an open-type microchip, COWFISH2A+ enables diverse assays at low cost through direct liquid deposition. As a proof of concept, we demonstrate two types of digital bioanalysis: a digital enzyme assay and an amplification-free digital genetic assay, SATORI. The digital enzyme assay discriminates alkaline phosphatase, a blood biomarker, at the single-isozyme level with sub-femtomolar sensitivity, while SATORI achieves attomolar-level detection of multiple pathogen-derived genetic targets. Furthermore, to demonstrate the translational potential of SATORI, we directly test 159 clinical specimens, detecting SARS-CoV-2 from nasopharyngeal swabs within ∼20 min with 92% sensitivity and 85% specificity. COWFISH2A+ thus provides an accessible and versatile platform for digital bioanalysis.

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Cite

BibTeXRIS
BibTeX
@article{iida2026cowfish2a,
  title = {{COWFISH2A+ as a versatile and accessible platform for digital bioanalysis}},
  author = {Tatsuya Iida and Hajime Shinoda and Mami Yoshimura and Asami Makino and Taichi Yoshimura and Masaharu Nakazawa and Hiroshi Hayakawa and Yasushi Kogo and Kosuke Miyauchi and Takeshi Noda and Toshiaki Ban and Hiroaki Takeuchi and Masashi Toyoda and Rikiya Watanabe},
  journal = {Cell Reports Physical Science},
  year = {2026},
  month = oct,
  doi = {10.1016/j.xcrp.2026.103587},
  url = {https://doi.org/10.1016/j.xcrp.2026.103587},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - COWFISH2A+ as a versatile and accessible platform for digital bioanalysis
AU  - Tatsuya Iida
AU  - Hajime Shinoda
AU  - Mami Yoshimura
AU  - Asami Makino
AU  - Taichi Yoshimura
AU  - Masaharu Nakazawa
AU  - Hiroshi Hayakawa
AU  - Yasushi Kogo
AU  - Kosuke Miyauchi
AU  - Takeshi Noda
AU  - Toshiaki Ban
AU  - Hiroaki Takeuchi
AU  - Masashi Toyoda
AU  - Rikiya Watanabe
PY  - 2026
DA  - 2026/10/23
JO  - Cell Reports Physical Science
DO  - 10.1016/j.xcrp.2026.103587
UR  - https://doi.org/10.1016/j.xcrp.2026.103587
AB  - Digital bioanalysis enables single-molecule detection of biomolecules and holds transformative potential across the life sciences and clinical diagnostics. However, its broader translation remains limited by accessibility and versatility. Here, we present COWFISH2A+, a compact, fully automated device integrating wide-field imaging with a custom dispensing module. Using an open-type microchip, COWFISH2A+ enables diverse assays at low cost through direct liquid deposition. As a proof of concept, we demonstrate two types of digital bioanalysis: a digital enzyme assay and an amplification-free digital genetic assay, SATORI. The digital enzyme assay discriminates alkaline phosphatase, a blood biomarker, at the single-isozyme level with sub-femtomolar sensitivity, while SATORI achieves attomolar-level detection of multiple pathogen-derived genetic targets. Furthermore, to demonstrate the translational potential of SATORI, we directly test 159 clinical specimens, detecting SARS-CoV-2 from nasopharyngeal swabs within ∼20 min with 92% sensitivity and 85% specificity. COWFISH2A+ thus provides an accessible and versatile platform for digital bioanalysis.
SN  - 2666-3864
ER  -