Cross-species atlas of bacterial RNA 3′ ends reveals termination mechanistic signatures and evolutionary patterns
Abstract
Deciphering the generation mechanisms and regulatory roles of bacterial transcript 3′ termini requires large-scale 3′ terminus data. We repurpose PATHOgenex, an available RNAtag-seq dataset, leveraging RNAtag-seq-specific read patterns associated with 3′ termini to generate the “Bacterminome” atlas, a rich resource of species- and condition-dependent transcript 3′ termini of 32 bacterial human pathogens grown under 10–12 conditions. Analysis of sequence elements near 3′ termini revealed, along with the classical intrinsic terminator, other known and newly detected signatures, highlighting the wealth of termination-associated sequence elements. Intriguingly, orthologous genes with corresponding 3′ termini show different termination mechanisms in different bacteria, often matching the dominant mechanism of the respective bacterial species. Analysis of condition-dependent 3′ termini revealed some conservation across species, supporting the conjecture that alternative 3′ termini can play a role in gene expression regulation. Our resource, available through a user-friendly web server (https://bacterminome.huji.ac.il), provides a platform for studying transcription termination at an unprecedented scope.
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BibTeX
@article{bar2026crossspecies,
title = {{Cross-species atlas of bacterial RNA 3′ ends reveals termination mechanistic signatures and evolutionary patterns}},
author = {Amir Bar and Yael Altuvia and Michal Eldar and Hanah Margalit},
journal = {Cell Reports},
year = {2026},
month = oct,
doi = {10.1016/j.celrep.2026.118076},
url = {https://linkinghub.elsevier.com/retrieve/pii/S2211124726011551},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Cross-species atlas of bacterial RNA 3′ ends reveals termination mechanistic signatures and evolutionary patterns
AU - Amir Bar
AU - Yael Altuvia
AU - Michal Eldar
AU - Hanah Margalit
PY - 2026
DA - 2026/10/01
JO - Cell Reports
DO - 10.1016/j.celrep.2026.118076
UR - https://linkinghub.elsevier.com/retrieve/pii/S2211124726011551
AB - Deciphering the generation mechanisms and regulatory roles of bacterial transcript 3′ termini requires large-scale 3′ terminus data. We repurpose PATHOgenex, an available RNAtag-seq dataset, leveraging RNAtag-seq-specific read patterns associated with 3′ termini to generate the “Bacterminome” atlas, a rich resource of species- and condition-dependent transcript 3′ termini of 32 bacterial human pathogens grown under 10–12 conditions. Analysis of sequence elements near 3′ termini revealed, along with the classical intrinsic terminator, other known and newly detected signatures, highlighting the wealth of termination-associated sequence elements. Intriguingly, orthologous genes with corresponding 3′ termini show different termination mechanisms in different bacteria, often matching the dominant mechanism of the respective bacterial species. Analysis of condition-dependent 3′ termini revealed some conservation across species, supporting the conjecture that alternative 3′ termini can play a role in gene expression regulation. Our resource, available through a user-friendly web server (https://bacterminome.huji.ac.il), provides a platform for studying transcription termination at an unprecedented scope.
SN - 2211-1247
ER -