Transcription factor CCTF1 plays an important role in regulating proteasome activity during archaeal cell division
Abstract
Archaea of the order Sulfolobales exhibit a highly ordered cell cycle progression similar to that of eukaryotes. It has been demonstrated that degradation of the cell division protein CdvB by the proteasome controls the progression of cell division. However, how the proteasome itself is regulated during the cell cycle is not fully understood. Recently, the cyclically expressed ArsR (arsenical resistance operon repressor) family transcription factor CCTF1 (cell cycle transcription factor 1) was shown to repress expression of PAN (proteasome-activating nucleotidase) in Sulfolobus acidocaldarius. Here, using biochemical approaches, comparative transcriptomics, and proteomics, we provide further insights into the CCTF1 function and show that it regulates PAN expression in Saccharolobus islandicus by binding to an AT-rich palindromic sequence within the pan promoter. We also reassessed the role of the cyclically expressed protein kinase aCcrK in the regulation of proteasome activity and conclude that CCTF1, rather than aCcrK, is the primary factor controlling the proteasome activity. These findings advance our understanding of the proteasome-mediated cell cycle regulation in archaea.
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BibTeX
@article{yang2026transcription,
title = {{Transcription factor CCTF1 plays an important role in regulating proteasome activity during archaeal cell division}},
author = {Yunfeng Yang and Zixin Geng and Shikuan Liang and Haodun Li and Fan Zhou and Junfeng Liu and Mart Krupovic and Yulong Shen},
journal = {Nucleic Acids Research},
year = {2026},
month = nov,
doi = {10.1093/nar/gkag1142},
url = {https://pasteur.hal.science/pasteur-05854147v1},
note = {CC BY-NC 4.0},
}
RIS
TY - JOUR
TI - Transcription factor CCTF1 plays an important role in regulating proteasome activity during archaeal cell division
AU - Yunfeng Yang
AU - Zixin Geng
AU - Shikuan Liang
AU - Haodun Li
AU - Fan Zhou
AU - Junfeng Liu
AU - Mart Krupovic
AU - Yulong Shen
PY - 2026
DA - 2026/11/05
JO - Nucleic Acids Research
DO - 10.1093/nar/gkag1142
UR - https://pasteur.hal.science/pasteur-05854147v1
AB - Archaea of the order Sulfolobales exhibit a highly ordered cell cycle progression similar to that of eukaryotes. It has been demonstrated that degradation of the cell division protein CdvB by the proteasome controls the progression of cell division. However, how the proteasome itself is regulated during the cell cycle is not fully understood. Recently, the cyclically expressed ArsR (arsenical resistance operon repressor) family transcription factor CCTF1 (cell cycle transcription factor 1) was shown to repress expression of PAN (proteasome-activating nucleotidase) in Sulfolobus acidocaldarius. Here, using biochemical approaches, comparative transcriptomics, and proteomics, we provide further insights into the CCTF1 function and show that it regulates PAN expression in Saccharolobus islandicus by binding to an AT-rich palindromic sequence within the pan promoter. We also reassessed the role of the cyclically expressed protein kinase aCcrK in the regulation of proteasome activity and conclude that CCTF1, rather than aCcrK, is the primary factor controlling the proteasome activity. These findings advance our understanding of the proteasome-mediated cell cycle regulation in archaea.
SN - 0305-1048
SN - 1362-4962
ER -