PreprintCC BY-NC-NDbioRxiv
Protocol for Single-Cell Patch-Proteomics: identify specific proteins associated with electrophysiological properties from individual neurons
- 1University of Michigan
Abstract
Single-cell patch-proteomics is a novel and powerful technique combining electrophysiological and quantitative proteomic characterization from individually sampled neurons. Following whole-cell patch clamp characterization of neuronal excitability, we collect intracellular contents and, using the latest advancements in Liquid Chromatography and Mass Spectrometry, identify and quantify peptides and their corresponding proteins. By cell-matched one-to-one associations between electrophysiological properties and protein abundance, this approach provides a means to elucidate molecular mechanisms of neuronal function on individual neuron basis.
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BibTeX
@misc{althaus2026protocol,
title = {{Protocol for Single-Cell Patch-Proteomics: identify specific proteins associated with electrophysiological properties from individual neurons}},
author = {J. C. Althaus and Y. Cao and Y. Song and A. Haghani and R. Callaghan and S. J. Moore and S. J. Parker and C. C. Kaczorowski},
howpublished = {bioRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.24.750455},
url = {https://www.biorxiv.org/content/10.64898/2026.09.24.750455v1},
note = {CC BY-NC-ND},
}
RIS
TY - UNPB
TI - Protocol for Single-Cell Patch-Proteomics: identify specific proteins associated with electrophysiological properties from individual neurons
AU - J. C. Althaus
AU - Y. Cao
AU - Y. Song
AU - A. Haghani
AU - R. Callaghan
AU - S. J. Moore
AU - S. J. Parker
AU - C. C. Kaczorowski
PY - 2026
DA - 2026/09/24
PB - bioRxiv
DO - 10.64898/2026.09.24.750455
UR - https://www.biorxiv.org/content/10.64898/2026.09.24.750455v1
AB - Single-cell patch-proteomics is a novel and powerful technique combining electrophysiological and quantitative proteomic characterization from individually sampled neurons. Following whole-cell patch clamp characterization of neuronal excitability, we collect intracellular contents and, using the latest advancements in Liquid Chromatography and Mass Spectrometry, identify and quantify peptides and their corresponding proteins. By cell-matched one-to-one associations between electrophysiological properties and protein abundance, this approach provides a means to elucidate molecular mechanisms of neuronal function on individual neuron basis.
ER -