Dynamics of Drosophila immune stress hematopoiesis assessed by single cell transcriptomics
Abstract
The molecular complexity of the hematopoietic microenvironment (niche) and its response to immune stress remain largely unknown. Here, we establish the transcriptional profile of the lymph gland, the Drosophila hematopoietic organ, and its associated niche, the posterior signaling center, at single-cell resolution and at different time points following an immune challenge. Among blood cells, we identify permanent cellular states present with and without stress, but also transient states specific to the immune response, which correspond to dividing cells primed to differentiate into dedicated effector cells. The niche itself is a heterogeneous cell population, and most niche cells undergo dynamic transcriptional remodeling following immune stress. Finally, we identify two new players involved in the hematopoietic stress response: the immune deficiency pathway and the cholecystokinin-like receptor, both of which are expressed in niche cell subsets during immune stress conditions. This molecular atlas of lymph gland cells at different time points after an immune challenge provides new insights into the control of stress hematopoiesis by discrete niche cell subsets
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BibTeX
@article{morinpoulard2026dynamics,
title = {{Dynamics of Drosophila immune stress hematopoiesis assessed by single cell transcriptomics}},
author = {Ismaël Morin-Poulard and Xiaohui Liu and Yushun Tian and Marianne Montemurro and Linna Sheng and Delphine Labourdette and Nathalie Vanzo and Michèle Crozatier},
journal = {Frontiers in Immunology},
year = {2026},
month = nov,
doi = {10.3389/fimmu.2026.1988884},
url = {https://www.frontiersin.org/articles/10.3389/fimmu.2026.1988884},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Dynamics of Drosophila immune stress hematopoiesis assessed by single cell transcriptomics
AU - Ismaël Morin-Poulard
AU - Xiaohui Liu
AU - Yushun Tian
AU - Marianne Montemurro
AU - Linna Sheng
AU - Delphine Labourdette
AU - Nathalie Vanzo
AU - Michèle Crozatier
PY - 2026
DA - 2026/11/03
JO - Frontiers in Immunology
DO - 10.3389/fimmu.2026.1988884
UR - https://www.frontiersin.org/articles/10.3389/fimmu.2026.1988884
AB - The molecular complexity of the hematopoietic microenvironment (niche) and its response to immune stress remain largely unknown. Here, we establish the transcriptional profile of the lymph gland, the Drosophila hematopoietic organ, and its associated niche, the posterior signaling center, at single-cell resolution and at different time points following an immune challenge. Among blood cells, we identify permanent cellular states present with and without stress, but also transient states specific to the immune response, which correspond to dividing cells primed to differentiate into dedicated effector cells. The niche itself is a heterogeneous cell population, and most niche cells undergo dynamic transcriptional remodeling following immune stress. Finally, we identify two new players involved in the hematopoietic stress response: the immune deficiency pathway and the cholecystokinin-like receptor, both of which are expressed in niche cell subsets during immune stress conditions. This molecular atlas of lymph gland cells at different time points after an immune challenge provides new insights into the control of stress hematopoiesis by discrete niche cell subsets
SN - 1664-3224
ER -