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Peer-reviewedCC BY 4.0Advanced Science

Uhrf1‐Mediated PKM2 Degradation via Ubiquitination Alleviates Inflammation and Pyroptosis in Inflammatory Bowel Disease

  • Juan Zhang1
  • Xin‐Rong Xu1
  • Hui‐Lin Zhang1
  • Qi‐Meng Zhu1
  • Xin‐Yuan Li1
  • Xi‐Peng Shang2
  • Jing‐Hui Du2
  • Christophe Morisseau3
  • Feng Qiu1
  • Cheng‐Peng Sun1
  1. 1School of Chinese Materia Medica School of Medical Technology Tianjin Key Laboratory of Therapeutic Substance of Traditional Chinese Medicine Tianjin University of Traditional Chinese Medicine Tianjin People's Republic of China
  2. 2First Teaching Hospital of Tianjin University of Traditional Chinese Medicine Tianjin People's Republic of China
  3. 3Department of Entomology and Nematology UC Davis Comprehensive Cancer Center University of California Davis California USA
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Abstract

Inflammatory bowel disease (IBD) is characterized by chronic relapsing intestinal inflammation and closely associated with persistent inflammatory responses and impairment of the mucosal barrier integrity. In this work, we demonstrated that Th17/Treg immune imbalance drove inflammation and pyroptosis in IBD, as revealed by single‐cell RNA sequencing, and pyruvate kinase M2 (PKM2) and ubiquitin‐like with PHD and RING finger domains 1‌ (Uhrf1) displayed a negative correlation in IBD clinic samples. Target fishing analysis revealed that eupatolide (EPT) covalently bound to C165 of PKM2 with a dissociation constant (K d) of 106 nM, corroborated by follow‐up chemical biology assays. We gained a deeper understanding of the mechanistic by which EPT interfered PKM2 function, specifically by promoting its interaction with Uhrf1, enhancing the K48‐linked ubiquitylation and degradation of PKM2 to block its nuclear translocation. Critically, PKM2 silencing mitigated dextran sodium sulfate (DSS)‐driven inflammation and pyroptosis across in vitro and in vivo models, with EPT showing no further efficacy upon PKM2 knockdown in IBD mice. This work uncovered Uhrf1‐dependent PKM2 ubiquitination/degradation as a previously unrecognized therapeutic axis for IBD, while positioning EPT as a molecular glue capable of targeting the Uhrf1–PKM2 complex.

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Cite

BibTeXRIS
BibTeX
@article{zhang2026uhrf1mediated,
  title = {{Uhrf1‐Mediated PKM2 Degradation via Ubiquitination Alleviates Inflammation and Pyroptosis in Inflammatory Bowel Disease}},
  author = {Juan Zhang and Xin‐Rong Xu and Hui‐Lin Zhang and Qi‐Meng Zhu and Xin‐Yuan Li and Xi‐Peng Shang and Jing‐Hui Du and Christophe Morisseau and Feng Qiu and Cheng‐Peng Sun},
  journal = {Advanced Science},
  year = {2026},
  month = aug,
  doi = {10.1002/advs.77403},
  url = {https://doi.org/10.1002/advs.77403},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Uhrf1‐Mediated PKM2 Degradation via Ubiquitination Alleviates Inflammation and Pyroptosis in Inflammatory Bowel Disease
AU  - Juan Zhang
AU  - Xin‐Rong Xu
AU  - Hui‐Lin Zhang
AU  - Qi‐Meng Zhu
AU  - Xin‐Yuan Li
AU  - Xi‐Peng Shang
AU  - Jing‐Hui Du
AU  - Christophe Morisseau
AU  - Feng Qiu
AU  - Cheng‐Peng Sun
PY  - 2026
DA  - 2026/08/25
JO  - Advanced Science
DO  - 10.1002/advs.77403
UR  - https://doi.org/10.1002/advs.77403
AB  - Inflammatory bowel disease (IBD) is characterized by chronic relapsing intestinal inflammation and closely associated with persistent inflammatory responses and impairment of the mucosal barrier integrity. In this work, we demonstrated that Th17/Treg immune imbalance drove inflammation and pyroptosis in IBD, as revealed by single‐cell RNA sequencing, and pyruvate kinase M2 (PKM2) and ubiquitin‐like with PHD and RING finger domains 1‌ (Uhrf1) displayed a negative correlation in IBD clinic samples. Target fishing analysis revealed that eupatolide (EPT) covalently bound to C165 of PKM2 with a dissociation constant (K d) of 106 nM, corroborated by follow‐up chemical biology assays. We gained a deeper understanding of the mechanistic by which EPT interfered PKM2 function, specifically by promoting its interaction with Uhrf1, enhancing the K48‐linked ubiquitylation and degradation of PKM2 to block its nuclear translocation. Critically, PKM2 silencing mitigated dextran sodium sulfate (DSS)‐driven inflammation and pyroptosis across in vitro and in vivo models, with EPT showing no further efficacy upon PKM2 knockdown in IBD mice. This work uncovered Uhrf1‐dependent PKM2 ubiquitination/degradation as a previously unrecognized therapeutic axis for IBD, while positioning EPT as a molecular glue capable of targeting the Uhrf1–PKM2 complex.
SN  - 2198-3844
ER  -