Role of cervicovaginal microbiome and metabolome in ALA-PDT-mediated HPV clearance in patients with cervical HSIL.
6 affiliations
- 1Department of Gynecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
- 2Henan Cervical Disease Prevention and Control Engineering Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
- 3Department of Gynecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. [email protected].
- 4Henan Cervical Disease Prevention and Control Engineering Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. [email protected].
- 5Department of Gynecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. [email protected].
- 6Henan Cervical Disease Prevention and Control Engineering Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. [email protected].
Abstract
The cervicovaginal microbiome and its associated metabolites play a pivotal role in the persistence of human papillomavirus (HPV) infection. Here, we investigated the contribution of the cervicovaginal microbiome and metabolome to 5-aminolevulinic acid photodynamic therapy (ALA-PDT)-mediated HPV clearance in patients with cervical high-grade squamous intraepithelial lesions (HSIL). 16S ribosomal RNA sequencing analysis identified significant differences in cervicovaginal microbiota before ALA-PDT between the HPV clearance and non-clearance groups. The HPV non-clearance group exhibited higher abundances of Anaerococcus and Gemella. ALA-PDT exerted a more substantial impact on cervicovaginal microbiota diversity in the HPV clearance group, characterized by increased abundances of Finegoldia, Corynebacterium, Lancefieldella, and Winkia, and a decreased abundance of Anaeroglobus following treatment. Global untargeted metabolomics analysis further revealed distinct cervicovaginal metabolic profiles before ALA-PDT between the two groups. The pathways of mineral absorption and valine, leucine, and isoleucine biosynthesis were significantly enriched in the HPV clearance group. Alterations in cervicovaginal metabolic profiles following ALA-PDT were also observed. Moreover, several key differential metabolites were observed to be significantly correlated with Anaeroglobus. Our findings underscore the critical involvement of the cervicovaginal microbiome and metabolome in ALA-PDT-mediated HPV clearance, providing valuable clues for predicting and improving the effect of HPV clearance following ALA-PDT.
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BibTeX
@article{zhang2026role,
title = {{Role of cervicovaginal microbiome and metabolome in ALA-PDT-mediated HPV clearance in patients with cervical HSIL.}},
author = {Ting Zhang and Chunyan Zhang and Shuyu Yuan and Yimeng Wang and Mengzhuo Zhang and Kaili Dai and Yiduo Yu and Lijuan Zhang and Lili Cao and Mengzhen Zhang and Yuehui Su},
journal = {NPJ biofilms and microbiomes},
year = {2026},
month = jul,
doi = {10.1038/s41522-026-01120-6},
url = {https://europepmc.org/article/MED/42897152},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Role of cervicovaginal microbiome and metabolome in ALA-PDT-mediated HPV clearance in patients with cervical HSIL.
AU - Ting Zhang
AU - Chunyan Zhang
AU - Shuyu Yuan
AU - Yimeng Wang
AU - Mengzhuo Zhang
AU - Kaili Dai
AU - Yiduo Yu
AU - Lijuan Zhang
AU - Lili Cao
AU - Mengzhen Zhang
AU - Yuehui Su
PY - 2026
DA - 2026/07/01
JO - NPJ biofilms and microbiomes
DO - 10.1038/s41522-026-01120-6
UR - https://europepmc.org/article/MED/42897152
AB - The cervicovaginal microbiome and its associated metabolites play a pivotal role in the persistence of human papillomavirus (HPV) infection. Here, we investigated the contribution of the cervicovaginal microbiome and metabolome to 5-aminolevulinic acid photodynamic therapy (ALA-PDT)-mediated HPV clearance in patients with cervical high-grade squamous intraepithelial lesions (HSIL). 16S ribosomal RNA sequencing analysis identified significant differences in cervicovaginal microbiota before ALA-PDT between the HPV clearance and non-clearance groups. The HPV non-clearance group exhibited higher abundances of Anaerococcus and Gemella. ALA-PDT exerted a more substantial impact on cervicovaginal microbiota diversity in the HPV clearance group, characterized by increased abundances of Finegoldia, Corynebacterium, Lancefieldella, and Winkia, and a decreased abundance of Anaeroglobus following treatment. Global untargeted metabolomics analysis further revealed distinct cervicovaginal metabolic profiles before ALA-PDT between the two groups. The pathways of mineral absorption and valine, leucine, and isoleucine biosynthesis were significantly enriched in the HPV clearance group. Alterations in cervicovaginal metabolic profiles following ALA-PDT were also observed. Moreover, several key differential metabolites were observed to be significantly correlated with Anaeroglobus. Our findings underscore the critical involvement of the cervicovaginal microbiome and metabolome in ALA-PDT-mediated HPV clearance, providing valuable clues for predicting and improving the effect of HPV clearance following ALA-PDT.
SN - 2055-5008
ER -